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Strain Detail Sheet

Strain Name:
B6J.129S7-Pamtm1Jep/Mmjax
Stock Number:
043561-JAX
Citation ID:
RRID:MMRRC_043561-JAX
Other Names:
C57 PAMhet

Strain Information

Gene Details

Pamtm1Jep
Name: peptidylglycine alpha-amidating monooxygenase; targeted mutation 1, John E Pintar
Synonyms: PAM-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: Chr1:97795114-98095646 bp
Alteration at locus: Knockout
Pam
Name: peptidylglycine alpha-amidating monooxygenase
Synonyms: PHM
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 18484
HGNC: HGNC:8596
Homologene: 37369

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Deletion of exons 2 and 3 of Pam (Gene ID:18484), which includes the starting methionine, signal sequence, and part of the first copper binding site.
Genotype Determination
ES Cell Line
AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2

Strain Description

Phenotype

Homozygous: Embryonic lethal at E14.5

Hetero/Hemizygous: PAM het is anxious, cannot handle cold room (core temp down 5C in 2 h), overly sensitive to cocaine, overly sensitive to seizure-producing drugs, poor glucose tolerance at 10 months age

Cre-excised Phenotype: Undetermined

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
Strain Development
Bred into C57BL/6J (Jackson Stock #000664), fresh WT breeding stock introduced 2-3 times per year for 12 years.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Cell Biology
  • Diabetes
  • Endocrine Deficiency
  • Metabolism
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor
John Pintar, Ph.D., Rutgers Robert Wood Johnson Medical School.

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
black

Breeding

MMRRC Breeding System
Other or uncertain
Breeding Scheme(s)
heterozygote males bred with fresh JAX females
Generation
>50
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 8 to 12 months
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.