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Availability & Fees Order this Strain
Homozygous: In the absence of FcRI, cellular processes induced by IgG2a-IC are impaired including phagocytosis, cytokine release, cellular cytotoxicity, and antigen presentation. FcRI-/- mice also showed impaired hypersensitivity responses. In an arthritis model, cartilage destructions is reduced and in a bacterial infection model, there is impaired protection from infection.
Hetero/Hemizygous: unclear
Cre-excised Phenotype:
Per Co-Donor:
"The targeting construct was generated in which part of the 3’ part of the promoter region, the L1 and L2 encoding exons, and the 5 part of the ligand binding EC1 domain encoding exon of the FcRI gene were replaced by the hygromycin resistance gene. The construct was electroporated into E14 embryonic stem (ES) cells, the targeted ES clones injected into day 3.5 C57BL/6 blastocysts, and the resulting chimeras bred to Balb/c females. Homozygous FcRI-/- mice developed normally and were fertile. Mice were originally bred to Balb/c (Charles River) but later bred to C57BL/6. We obtained the B6.FcgRI-/- mice from Ann Sperling with permission from JS Verbeek."
Additional detail per donor:
"The targeting was performed in E14 129 derived ES cells and the mouse was backcrosses on BALB/c two generations to obtain homozygous KOs. This appeared impossible with direct backcrossing into C57B/6. Mark Hogarth who generated also a FcgRI KO faced the same problem (his FcgRI KO mouse is back to back published with ours in Immunity). After two generations BALB/c we backcrossed again into C57BL6Jico and surprisingly we got homozygous KOs. We continued backcrossing 14 times. In conclusion it cannot be excluded that this mouse contains some BALB/c genomic sequences. Certainly the sequences flanking the FcgRI KO allele are 129 derived (PMID:26163370)."
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.