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Strain Name:
CBA;B6J-Tg(Thy1-KCNB1*C73A)18Sesf/Mmmh
Stock Number:
043830-MU
Citation ID:
RRID:MMRRC_043830-MU
Other Names:
Tg-C73A, pThy-KCNB1C73A-HA

Strain Information

Tg(Thy1-KCNB1*C73A)18Sesf
Name: transgene insertion 18, Federico Sesti
Synonyms: Tg-C73A
Type: Allele
Species: Multi-species
Chromosome: random insertion
Alteration at locus: Transgenic (random, gene disruption)
Thy1
Name: thymus cell antigen 1, theta
Synonyms: CD90, Thy-1, Thy 1.2, Thy1.1, Thy-1.2, Thy1.2, T25
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Transgenic (random, gene disruption)
NCBI: 21838
Homologene: 4580
HA
Name: haemagglutinin, Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Type: Gene
Species: Influenza virus
Chromosome:
Alteration at locus: Transgenic (random, gene disruption)
KCNB1
Name: potassium voltage-gated channel subfamily B member 1
Type: Gene
Species: Homo sapiens (human)
Chromosome: 20
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations

Random insertion of Thy1-driven human KCNB1 (human voltage-gated K+ channel subfamily B, member 1 - Gene ID:3745) for cortical/hippocampal expression. The channel is C-terminally HA-tagged and it carries a substitution:

HGVS nomenclature:

  • Genbank RefSeq - mRNA: NM_004975.4
  • Genbank RefSeq, protein: NP_004966.1
  • Variant, nucleic acid level: c.217_218delinsGC
  • Variant, amino acid level, predicted: p.Cys73Ala
    Check this variant: LUMC Mutalyzer
Genotype Determination
Phenotype

Homozygous: not tested

Hetero/Hemizygous: mice develop normally and exhibit improved cognitive function in the Morris water maze with age compared to age-matched non-transgenic mice

MeSH Terms
  • 2,2'-Dipyridyl/analogs & derivatives
  • 2,2'-Dipyridyl/toxicity
  • Age Factors
  • Alanine/genetics
  • Alzheimer Disease/genetics
  • Alzheimer Disease/pathology
  • Alzheimer Disease/physiopathology
  • Amyloid beta-Peptides/toxicity
  • Amyloid beta-Protein Precursor/genetics
  • Analysis of Variance
  • Animals
  • Animals, Genetically Modified
  • Apoptosis/drug effects
  • Apoptosis/genetics
  • Apoptosis/physiology
  • Brain/cytology
  • Caenorhabditis elegans
  • Cells, Cultured
  • Cricetinae
  • Cricetulus
  • Cysteine/genetics
  • Disease Models, Animal
  • Disulfides/toxicity
  • Electric Stimulation
  • Embryo, Mammalian
  • Female
  • Fluoresceins/pharmacology
  • Humans
  • Hydrogen Peroxide/pharmacology
  • Male
  • Mass Spectrometry/methods
  • Membrane Potentials/genetics
  • Membrane Potentials/physiology
  • Mice
  • Neurons/drug effects
  • Neurons/physiology
  • Oxidants/toxicity
  • Oxidation-Reduction/drug effects
  • Oxidative Stress/drug effects
  • Oxidative Stress/genetics
  • Oxidative Stress/physiology
  • Patch-Clamp Techniques
  • Peptide Fragments/toxicity
  • Presenilin-1/genetics
  • Propanols/pharmacology
  • Shab Potassium Channels/genetics
  • Shab Potassium Channels/physiology
  • Transfection
  • Apoptosis
  • Brain Injuries, Traumatic/pathology
  • Brain Injuries, Traumatic/physiopathology
  • Cognition Disorders/pathology
  • Cognition Disorders/physiopathology
  • Dasatinib/administration & dosage
  • Hippocampus/pathology
  • Hippocampus/physiopathology
  • Mice, Transgenic
  • Neurodegenerative Diseases
  • Neurons/metabolism
  • Neurons/pathology
  • Protein Kinase Inhibitors/administration & dosage
  • Reactive Oxygen Species/metabolism
  • Shab Potassium Channels/metabolism
  • Amino Acid Substitution
  • Brain/metabolism
  • CHO Cells
  • Focal Adhesion Kinase 1/genetics
  • Focal Adhesion Kinase 1/metabolism
  • Integrin alpha5/genetics
  • Integrin alpha5/metabolism
  • Mutation, Missense
  • Proto-Oncogene Proteins c-fyn/genetics
  • Proto-Oncogene Proteins c-fyn/metabolism
  • Signal Transduction/physiology
  • src-Family Kinases/genetics
  • src-Family Kinases/metabolism
Strain Development
The line was created by the Rutgers University Genome Editing Core Facility, using pronuclear microinjection of C57BL/6J with subsequent crossing to CBA mice to generate transgenic lines in the B6CBAF1/J background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Neurobiology
Donor
Federico Sesti, Ph.D., Rutgers University Biomedical and Health Sciences.
Primary Reference

Cotella D, Hernandez-Enriquez B, Wu X, Li R, Pan Z, Leveille J, Link CD, Oddo S, Sesti F. Toxic role of K+ channel oxidation in mammalian brain. J Neurosci. 2012 Mar 21;32(12):4133-44. doi: 10.1523/JNEUROSCI.6153-11.2012. Erratum in: J Neurosci. 2012 Jul 4;32(27):9449. (Medline PMID: 22442077)

Yu W, Parakramaweera R, Teng S, Gowda M, Sharad Y, Thakker-Varia S, Alder J,Sesti F. Oxidation of KCNB1 Potassium Channels Causes Neurotoxicity and CognitiveImpairment in a Mouse Model of Traumatic Brain Injury. J Neurosci. 2016 Oct26;36(43):11084-11096. (Medline PMID: 27798188)

Yu W, Gowda M, Sharad Y, Singh SA, Sesti F. Oxidation of KCNB1 potassiumchannels triggers apoptotic integrin signaling in the brain. Cell Death Dis. 2017Apr 6;8(4):e2737. doi: 10.1038/cddis.2017.160. (Medline PMID: 28383553)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Not available
Eye
Not available
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
The donor routinely kept two harem cages with 1 male and 4 females.
Generation
Not available
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
No
Average litter size
Undetermined
Recommended wean age
3-4 weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043830-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
043830-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.