Strain Detail Sheet

Strain Name:
B6.129S4(Cg)-Apptm1.2Tlyp/Mmjax
Stock Number:
043842-JAX
Citation ID:
RRID:MMRRC_043842-JAX
Other Names:
APP gKO, APP germline knockout

Strain Information

Gene Details

Apptm1.2Tlyp
Name: amyloid beta precursor protein; targeted mutation 1.2, Tracy L Young-Pearse
Synonyms: APP gKO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
App
Name: amyloid beta precursor protein
Synonyms: Abeta, Adap, protease nexin II, betaAPP, E030013M08Rik, Cvap, appican
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Knockout
NCBI: 11820
VEGA: 16
HGNC: HGNC:620
Homologene: 56379
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Genetic Alterations
Germline knock-out App mice have a deletion of exon 3 of the amyloid beta (A4) precursor protein gene (App) - this eliminates APP protein expression in the cortex. These mice may be useful for studying APP function and Alzheimer's disease.
Genotype Determination
ES Cell Line
J1

Strain Description

Phenotype
Amyloid beta (A4) precursor protein (APP) is a type I integral membrane protein that is cleaved to form a number of peptides, in particular, the amyloid-β-peptide, a component of the amyloid plaques associated with Alzheimer's disease.

The germline knock-out App allele has exon 3 deleted. APP protein expression is eliminated in the cortex, however, cortical layer markers, astrocyte markers and microglial markers are not disrupted. Homozygous mice are viable and fertile. No gross morphological differences are observed between embryonic wild-type and knock-out brains. These mice may be useful for studying APP function.

The Appflox conditional knock-out allele (Apptm1.1Tlyp) is available as MMRRC Stock No. 031606.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
Strain Development
The targeting vector was designed to insert a loxP site in intron 2 and a frt-flanked neomycin selection cassette in intron 3 followed by a second loxP site. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric animals were crossed to C57BL/6 mice, and offspring were crossed to B6;SJL-Tg(ACTFLPe)9205Dym/J mice, and then crossed to BALB/c-Tg(CMV-cre)1Cgn/J to remove exon 3. Resulting mice with the germline knock-out App allele were selected and then backcrossed to C57BL/6J mice for at least five generations (and the FLPe- and Cre-expressing transgenes were removed). Upon arrival at The Jackson Laboratory Repository, mice were bred to C57BL/6J for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Donor
Tracy Young-Pearse, Ph.D., Brigham and Women's Hospital.

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
Complex breeding scheme: maintaining transgenic strain by mating heterozygotes or homozygotes with wild-type or heterozygote individuals and performing genotyping on individuals from litters
Generation
N≥5
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3 weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.