Strain Name:
STOCK Pdx1tm1Cvw/Mmnc
Stock Number:
000439-UNC
Citation ID:
RRID:MMRRC_000439-UNC

Gene Information

Allele Symbol: Pdx1tm1Cvw (Mus musculus (mouse))
Name: pancreatic and duodenal homeobox 1; targeted mutation 1, Christopher VE Wright
Synonyms: pdx-1 -, Ipf1<->, pdx
Chromosome: 5
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:2181360
Gene Symbol: Pdx1 (Mus musculus (mouse))
Name: pancreatic and duodenal homeobox 1
Synonyms: STF-1, IDX-1, IPF-1, Mody4, pdx-1, Ipf1
Chromosome: 5
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:102851
Genetic Alterations
Neo cassette inserted into exon 2 of Pdx1
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
    Phenotype
    Homozygous: A pancreatic mice due to a blockage in differentiation of endocrine and exocrine cell populations. Formation of pancreatic buds and limited ductal outgrowth and branching is observed.

    Heterozygous: similar to wild-type
    Mammalian Phenotype Terms
    Allelic Composition: Pdx1tm1Cvw/Pdx1tm1Cvw (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * Black Swiss )

    Allelic Composition: Pdx1tm1Cvw/Pdx1+ (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 )

    Allelic Composition: Pdx1tm1Cvw/Pdx1tm1.1Stof (Genetic Background: involves: 129/Sv * 129S1/Sv * 129X1/SvJ * C57BL/6 )

    MeSH Terms
    • Animals
    • Cholecystokinin/metabolism
    • Duodenum/embryology
    • Endoderm
    • Gene Expression Regulation, Developmental
    • Genes, Homeobox
    • Glucose Transporter Type 2
    • Heterozygote
    • Homeodomain Proteins
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Monosaccharide Transport Proteins/genetics
    • Pancreas/embryology
    • RNA, Messenger/genetics
    • Secretin/metabolism
    • Serotonin/metabolism
    • Trans-Activators/physiology
    Suggested Control Mice
    • Wildtype littermates
    • Developmental Biology
    • Diabetes/Obesity
    • Endocrine Deficiency
    • Research Tools
    Donor
    Chris V. Wright, Ph.D., Vanderbilt University
    Primary Reference
    Offield MF, Jetton TL, Labosky PA, Ray M, Stein RW, Magnuson MA, Hogan BL, Wright CV. PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum. Development. 1996 Mar;122(3):983-95. (Medline PMID: 8631275)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    000439-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.