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Strain Detail Sheet

Strain Name:
B6;129/Sv-Errfi1tm1.1Jwj/Mmnc
Stock Number:
044016-UNC
Citation ID:
RRID:MMRRC_044016-UNC
Other Names:
Mig-6(floxed/floxed)

Strain Information

Gene Details

Errfi1tm1.1Jwj
Name: ERBB receptor feedback inhibitor 1; targeted mutation 1.1, Jae-Wook Jeong
Synonyms: Errfid, Mig-6-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Conditional
Errfi1
Name: ERBB receptor feedback inhibitor 1
Synonyms: 1300002F13Rik, Mig-6, RALT
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Conditional
NCBI: 74155
Homologene: 10344

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Mig-6(floxed/floxed) mouse by introducing LoxP sites that flank exons 2 and 4 of Mig-6.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    R1

    Strain Description

    Phenotype
    None/Normal/Wild-type
    Mammalian Phenotype Terms
    Allelic Composition: (Genetic Background: )
    MeSH Terms
    • Adaptor Proteins, Signal Transducing/genetics
    • Adaptor Proteins, Signal Transducing/metabolism
    • Alleles
    • Animals
    • Cell Shape
    • Genetic Engineering/methods
    • Intracellular Signaling Peptides and Proteins
    • Mice
    • Mice, Knockout
    • Skin/cytology
    • Skin/metabolism
    • Adaptor Proteins, Signal Transducing/deficiency
    • Adolescent
    • Adult
    • Down-Regulation
    • Endometrial Hyperplasia/genetics
    • Endometrial Hyperplasia/metabolism
    • Endometrial Hyperplasia/pathology
    • Endometrial Neoplasms/genetics
    • Endometrial Neoplasms/metabolism
    • Endometrial Neoplasms/pathology
    • Estrogens/metabolism
    • Female
    • Histone Acetyltransferases/deficiency
    • Histone Acetyltransferases/genetics
    • Histone Acetyltransferases/metabolism
    • Humans
    • Nuclear Receptor Coactivator 1
    • Oligonucleotide Array Sequence Analysis
    • Progesterone/metabolism
    • Transcription Factors/deficiency
    • Transcription Factors/genetics
    • Transcription Factors/metabolism
    • Tumor Suppressor Proteins
    • Disease Progression
    • Extracellular Signal-Regulated MAP Kinases/metabolism
    • PTEN Phosphohydrolase/genetics
    • PTEN Phosphohydrolase/metabolism
    • Phosphorylation
    • Receptors, Progesterone/metabolism
    • Reverse Transcriptase Polymerase Chain Reaction
    • Tumor Suppressor Proteins/genetics
    • Tumor Suppressor Proteins/metabolism
    • Uterus/metabolism
    • Apoptosis/physiology
    • Baculoviral IAP Repeat-Containing 3 Protein
    • Cell Growth Processes/physiology
    • Endometrial Neoplasms/prevention & control
    • Epithelial Cells/metabolism
    • Epithelial Cells/pathology
    • Epithelium/metabolism
    • Epithelium/pathology
    • Estrogen Receptor alpha/genetics
    • Estrogen Receptor alpha/metabolism
    • Estrogens/genetics
    • Inhibitor of Apoptosis Proteins/genetics
    • Inhibitor of Apoptosis Proteins/metabolism
    • Intracellular Signaling Peptides and Proteins/genetics
    • Intracellular Signaling Peptides and Proteins/metabolism
    • Progesterone/genetics
    • Receptor, Notch1/genetics
    • Receptor, Notch1/metabolism
    • Receptors, Progesterone/genetics
    • Stromal Cells/metabolism
    • Stromal Cells/pathology
    • Ubiquitin-Protein Ligases
    • Uterus/pathology
    • Wnt Proteins/genetics
    • Wnt Proteins/metabolism
    • Chorda Tympani Nerve/physiology
    • Discrimination (Psychology)/drug effects
    • Ear, Inner/physiology
    • Male
    • Rats
    • Sensory Thresholds/drug effects
    • Sensory Thresholds/physiology
    • Sodium Chloride/pharmacology
    • Stereotaxic Techniques
    • Sucrose/pharmacology
    • Taste/physiology
    • Tympanic Membrane/physiology
    • Amino Acid Substitution/genetics
    • DNA Nucleotidyltransferases/genetics
    • DNA Nucleotidyltransferases/metabolism
    • Enzyme Stability
    • Genes, Reporter/genetics
    • Integrases/genetics
    • Integrases/metabolism
    • Mice, Transgenic
    • Mutagenesis, Site-Directed/genetics
    • Organ Specificity
    • Recombination, Genetic/genetics
    • Sequence Deletion/genetics
    • Transgenes/genetics
    • Viral Proteins
    Strain Development
    Errfi1 genomic DNA was isolated from R1 ES cells. To generate the targeting vector, the Errfi1 fragment with exon 2-4, 2-kb fragment of the 5' arm and 2-kb fragment of the 3' arm were subcloned into the TOPO-cloning vector (Invitrogen, Carlsbad, CA) using PCR reactions. High-fidelity Taq polymerase was used (Roche Applied Science, Indianapolis, IN) for PCR amplification, and all of the PCR products were confirmed by sequencing. One LoxP site followed by the PGK-neo cassette (phospho-glycerol kinase neomycin-resistance flanked with two FRT sites) was inserted downstream of exon 4. The other LoxP site was inserted upstream of exon 2. The 5' ends of the targeting vector were constructed by cloning 2kb of Errfi1 homologous sequence upstream of Mig-6 exon 2, whereas the 3' end contains a 2-kb homologous sequence downstream of Errfi1 exon 4. The linearized targeting construct was transfected into R1 ES cells by electroporation. Neomycin-resistant clones were screened by Southern-blot analysis using 5' external probes with EcoRI digested genomic DNA. Four of 192 clones were targeted. Correctly targeted clones were microinjected into blastocysts derived from C57BL/6 mice. Seven chimeras were bred to C57BL/6 mice, and F1 agouti offspring mice were analyzed by PCR genotyping to validate the germ line transmission of the Errfi1 floxneo allele (PMID:1798766). The neo cassette was removed with C57BL/6-Tg(ACTFLPe)9205Dym (MGI allele #2448985) strain obtained from the animal facility at Baylor College of Medicine, Houston TX.

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cancer
    • Diabetes
    • Models for Human Disease
    • Reproduction
    • Research Tools

    Strain Origin

    Donor
    Jae-wook Jeong, Ph.D Michigan State University
    Primary Reference

    Jin N, Gilbert JL, Broaddus RR, Demayo FJ, Jeong JW. Generation of a Mig-6conditional null allele. Genesis. 2007 Nov;45(11):716-21. (Medline PMID: 17987665)

    Jeong JW, Lee HS, Lee KY, White LD, Broaddus RR, Zhang YW, Vande Woude GF,Giudice LC, Young SL, Lessey BA, Tsai SY, Lydon JP, DeMayo FJ. Mig-6 modulatesuterine steroid hormone responsiveness and exhibits altered expression inendometrial disease. Proc Natl Acad Sci U S A. 2009 May 26;106(21):8677-82. doi: 10.1073/pnas.0903632106. Epub 2009 May 13. (Medline PMID: 19439667)

    Kim TH, Franco HL, Jung SY, Qin J, Broaddus RR, Lydon JP, Jeong JW. Thesynergistic effect of Mig-6 and Pten ablation on endometrial cancer developmentand progression. Oncogene. 2010 Jul 1;29(26):3770-80. doi: 10.1038/onc.2010.126. Epub 2010 Apr 26. (Medline PMID: 20418913)

    Kim TH, Lee DK, Cho SN, Orvis GD, Behringer RR, Lydon JP, Ku BJ, McCampbellAS, Broaddus RR, Jeong JW. Critical tumor suppressor function mediated byepithelial Mig-6 in endometrial cancer. Cancer Res. 2013 Aug 15;73(16):5090-9.doi: 10.1158/0008-5472.CAN-13-0241. Epub 2013 Jun 27. (Medline PMID: 23811943)

    Slotnick BM, Sheelar S, Rentmeister-Bryant H. Transection of the chordatympani and insertion of ear pins for stereotaxic surgery: equivalent effects on taste sensitivity. Physiol Behav. 1991 Dec;50(6):1123-7. (Medline PMID: 1798766)

    Rodríguez CI, Buchholz F, Galloway J, Sequerra R, Kasper J, Ayala R, StewartAF, Dymecki SM. High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP. Nat Genet. 2000 Jun;25(2):139-40. (Medline PMID: 10835623)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Generation
    F5+
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Average litter size
    7-9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    5-9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    044016-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.