Strain Name:
C57BL/6-Gt(ROSA)26Sortm1(lacZ*)Druss/Mmmh
Stock Number:
044026-MU
Citation ID:
RRID:MMRRC_044026-MU
Other Names:
ROSAnZ∆4

Strain Information

Gt(ROSA)26tm1(lacZ*)Druss
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, David Russell
Type: Allele
Species: Escherichia coli (bacterium)
Chromosome: 6
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910
lacZ
Name: beta galactosidase
Type: Gene
Species: Escherichia coli (bacterium)
Chromosome:
Genetic Alterations
The murine ROSA26 locus was targeted by homologous recombination with the pROSAnZD4 plasmid containing a recombinant lacZ gene with a central 4-bp deletion. The control strain (MMRRC:44049) was targeted with the pROSAnZ plasmid, which contains the wild-type lacZ gene.
Genotype Determination
ES Cell Line
C57BL/6-derived; see PMID:14713204
Phenotype
Normal phenotype and breeding. C57BL/6J-background ROSAnZ∆4 mice have a mutant lacZ gene with a central 4-bp deletion that can be corrected by any type of gene targeting, which includes bacterial plasmid sequences that facilitate recovery and sequence analysis of gene targeting events. This is a model for demonstrating in vivo gene targeting by any method by turning on lacZ expression, which can be observed in specific target tissues by blue color. The knock-in has nuclear restriction under control of ROSA26 promoter. The lacZD4 target sequence in ROSAnZ∆4 mice retains a prokaryotic transcriptional promoter that also allows the plasmids containing targeted lacZ sequences to be purified from mouse genomic DNA, electroporated into bacteria, and detected as blue colonies on medium supplemented with X-gal. The positive control line expressing a wild-type lacZ gene at the same locus (ROSAnZ) is MMRRC:44049.
MeSH Terms
  • Adenoviridae/genetics
  • Animals
  • Gene Targeting/methods
  • Genetic Engineering/methods
  • Genetic Vectors/genetics
  • Mice
  • Mice, Inbred C57BL
  • Transfection/methods
  • Cell Culture Techniques/methods
  • Cells, Cultured
  • Chimera
  • Embryo Transfer
  • Embryo, Mammalian/cytology
  • Female
  • Male
  • Mice, Inbred BALB C
  • Microinjections
  • Mutation
  • Stem Cells/cytology
Strain Development
Mouse strain C57BL/6-derived embryonic stem cells were electroporated with 10–20 mg of plasmids pROSAnZ or pROSAnZD4, linearized with SacII to generate transgenic mice. Transfected cells were selected in medium containing 0.3 mg/ml G418, and targeted clones were identified by Southern blot analysis. Several targeted clones were injected into BALB/c-derived blastocysts and implanted in pseudopregnant females (strain CD1). Chimeric mice were bred to C57BL/6J females and litters screened by coat color. Mice with black coats were checked by Southern blot analysis for the presence and structure of the targeted locus and bred to littermates to establish homozygosity for the targeted loci. Both ROSAnZ (MMRRC:44049) and ROSAnZD4 strains were maintained as homozygous breeding pairs that appeared healthy.
Suggested Control Mice
MMRRC:44049
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Research Tools
Donor
David Russell, Ph.D University of Washington
Primary Reference

Miller DG, Wang PR, Petek LM, Hirata RK, Sands MS, Russell DW. Gene targeting in vivo by adeno-associated virus vectors. Nat Biotechnol. 2006 Aug;24(8):1022-6.Epub 2006 Jul 30. (Medline PMID: 16878127)

Ware CB, Siverts LA, Nelson AM, Morton JF, Ladiges WC. Utility of a C57BL/6 ESline versus 129 ES lines for targeted mutations in mice. Transgenic Res. 2003Dec;12(6):743-6. (Medline PMID: 14713204)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
Other
This mouse is a useful model to demonstrate successful gene-therapy by gene-targeting in vivo. It can be used with any gene-editing method; CRISPR, lentiviral, foamy virus, rAAV, etc., by restoring the function of the deleted lacZ gene detecting the color in targeted tissues. The plasmid can be recovered from target tissues and grown up in bacteria.
MMRRC Breeding System
Random intra-strain mating
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044026-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
044026-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.