Strain Name:
B6.129P2-Cpt1cGt(XL823)Byg/Mmjax
Stock Number:
044037-JAX
Citation ID:
RRID:MMRRC_044037-JAX
Other Names:
CPT1C (gene trap)

Strain Information

Cpt1cGt(XL823)Byg
Name: carnitine palmitoyltransferase 1c; gene trap XL823, BayGenomics
Synonyms: Cpt1cgt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Gene Trap
Cpt1c
Name: carnitine palmitoyltransferase 1c
Synonyms: CPT I-C, 9630004I06Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Gene Trap
NCBI: 78070
Homologene: 17561
Genetic Alterations
A single gene trap vector (containing a splice acceptor site, β-Geo and a polyadenylation site) was inserted into intron 6 of the Cpt1c gene.
ES Cell Line
E14K
Phenotype
None/Normal/Wild-type
MeSH Terms
  • AMP-Activated Protein Kinases/metabolism
  • Adenosine Triphosphate/metabolism
  • Animals
  • Apoptosis/physiology
  • Carnitine O-Palmitoyltransferase/deficiency
  • Carnitine O-Palmitoyltransferase/genetics
  • Carnitine O-Palmitoyltransferase/metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Survival/genetics
  • Cells, Cultured
  • Drug Resistance, Neoplasm/genetics
  • Embryonic Stem Cells/enzymology
  • Gene Expression Regulation, Neoplastic
  • HCT116 Cells
  • Humans
  • Hypoxia/pathology
  • Lung Neoplasms/enzymology
  • Lung Neoplasms/pathology
  • Mice
  • RNA, Messenger/metabolism
  • Reproducibility of Results
  • Stress, Physiological/genetics
  • Stress, Physiological/physiology
  • TOR Serine-Threonine Kinases/metabolism
  • Transplantation, Heterologous
  • Up-Regulation
Strain Development
Embryonic stem (ES) cell line (clone XL823; Bay Genomics) carrying a single gene trap vector insertion into intron 6 of the Cpt1c gene was injected into C57BL/6 blastocysts to generate chimeric mice. Heterozygous mutant mice were generated by crossing chimeras to C57BL/6J mice and were subsequently backcrossed into C57BL/6J mice from Jackson (JAX Strain #000664).
Suggested Control Mice
Cpt1c+/+ and Cpt1c+/-
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Metabolism
Donor
Tak Mak, Ph.D Campbell Family Institute for Breast Cancer Research
Primary Reference

Zaugg K, Yao Y, Reilly PT, Kannan K, Kiarash R, Mason J, Huang P, Sawyer SK,Fuerth B, Faubert B, Kalliomäki T, Elia A, Luo X, Nadeem V, Bungard D, YalavarthiS, Growney JD, Wakeham A, Moolani Y, Silvester J, Ten AY, Bakker W, Tsuchihara K,Berger SL, Hill RP, Jones RG, Tsao M, Robinson MO, Thompson CB, Pan G, Mak TW.Carnitine palmitoyltransferase 1C promotes cell survival and tumor growth underconditions of metabolic stress. Genes Dev. 2011 May 15;25(10):1041-51. doi:10.1101/gad.1987211. (Medline PMID: 21576264)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
Other
Cpt1c may be an attractive target for therapeutic intervention where tumors are hypoxic and deprived of nutrient sources because cells can use a novel mechanism involving Cpt1c and fatty acid metabolism to protect against metabolic stress. Therefore, Cpt1c null mice could be a good tool to study those effects.
MMRRC Breeding System
Random intra-strain mating
Generation
N10
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Average litter size
4-6
Recommended wean age
3 Weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044037-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
044037-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.