Strain Name:
B6J.129P2-Traf6tm1Mak/Mmjax
Stock Number:
044044-JAX
Citation ID:
RRID:MMRRC_044044-JAX
Other Names:
Traf6 WT X +/- B6F10

Gene Information

Traf6tm1Mak
Name: TNF receptor-associated factor 6; targeted mutation 1, Tak Mak
Synonyms: TRAF6-
Type: Allele
Species: Multi-species
Chromosome: 2
Alteration at locus: Knock-In
Traf6
Name: TNF receptor-associated factor 6
Synonyms: 2310003F17Rik, C630032O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knock-In
NCBI: 22034
Homologene: 3395
Genetic Alterations
The targeting vector was engineered to replace the exon containing the ATG translation initiation codon with PGK-neo in the opposite orientation. This exon encodes the first 100 amino acids of Traf6, representing a portion of the amino-terminal ring-finger domain.
Genotype Determination
ES Cell Line
E14K derived from 129P2/OlaHsd
Phenotype
Mice deficient in TNF receptor-associated factor 6 (Traf6) are osteopetrotic with defects in bone remodeling and tooth eruption due to impaired osteoclast function. In vitro assays, Traf6 is crucial not only in IL-1 and CD40 signaling but also, surprisingly, in LPS signaling. Furthermore, like Traf2 and Traf3, Traf6 is essential for perinatal and postnatal survival. Thus Traf6 plays diverse and critical roles in perinatal and postnatal survival, bone metabolism, LPS, and cytokine signaling
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • B-Lymphocytes/metabolism
  • CD40 Antigens/metabolism
  • Calcium-Calmodulin-Dependent Protein Kinases/metabolism
  • Cell Division
  • Enzyme Activation
  • Female
  • Interleukin-1/metabolism
  • JNK Mitogen-Activated Protein Kinases
  • Lipopolysaccharides/metabolism
  • Lipopolysaccharides/pharmacology
  • Macrophages/enzymology
  • Mice
  • Mice, Knockout
  • Mitogen-Activated Protein Kinases
  • NF-kappa B/metabolism
  • Nitric Oxide Synthase/metabolism
  • Nitric Oxide Synthase Type II
  • Osteopetrosis/physiopathology
  • Proteins/genetics
  • Proteins/physiology
  • Signal Transduction
  • TNF Receptor-Associated Factor 6
Strain Development
The murine Traf6 gene was disrupted by introducing a targeted mutation into embryonic stem cells (E14K) by homologous recombination (see also: Genetic Alterations). Two independent heterozygous (TRAF6+/−) mutant ES cell lines were microinjected into C57BL/6 (B6) blastocysts, which were subsequently implanted into pseudopregnant CD-1 (ICR) recipients. Male chimeras generated from both cell lines were used to generate TRAF6+/− mice. TRAF6 +/- mice were backcrossed 10 times into C57BL/6 mice from Jackson (JAX Strain #000664).
Suggested Control Mice
Traf6+/+ AND Traf6+/- can be used as controls.
  • Cell Biology
  • Immunology and Inflammation
Donor
Tak Mak, Ph.D Cambell Family Institute for Breast Cancer Research
Primary Reference

Lomaga MA, Yeh WC, Sarosi I, Duncan GS, Furlonger C, Ho A, Morony S,Capparelli C, Van G, Kaufman S, van der Heiden A, Itie A, Wakeham A, Khoo W,Sasaki T, Cao Z, Penninger JM, Paige CJ, Lacey DL, Dunstan CR, Boyle WJ, Goeddel DV, Mak TW. TRAF6 deficiency results in osteopetrosis and defectiveinterleukin-1, CD40, and LPS signaling. Genes Dev. 1999 Apr 15;13(8):1015-24.(Medline PMID: 10215628)

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
N10
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Average litter size
4-6
Recommended wean age
3 Weeks
Average Pups Weaned
Less than 4

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044044-JAX-HET-F
044044-JAX-HET-M
044044-JAX-WT-F
044044-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$218.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
044044-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.