These mice are a positive control for ROSAnZ∆4 (MMRRC:44026), which have the mutated lacZ gene. They have a wild-type lacZ gene at the same locus as ROSAnZ∆4.

C57BL/6-derived; see PMID:14713204

Normal phenotype and breeding. Express expresses LacZ lacZ beta-D-galactosidase (Escherichia coli str. K-12 substr. MG1655). Compare to MMRRC:44026.

Mouse strain C57BL/6-derived embryonic stem cells were electroporated with 10–20 mg of plasmids pROSAnZ or pROSAnZ∆4, linearized with SacII to generate transgenic mice. Transfected cells were selected in medium containing 0.3 mg/ml G418, and targeted clones were identified by Southern blot analysis. Several targeted clones were injected into BALB/c-derived blastocysts and implanted in pseudopregnant females (strain CD1). Chimeric mice were bred to C57BL/6J females and litters screened by coat color. Mice with black coats were checked by Southern blot analysis for the presence and structure of the targeted locus and bred to littermates to establish homozygosity for the targeted loci. Both ROSAnZ (MMRRC:44049) and ROSAnZ∆4 strains were maintained as homozygous breeding pairs that appeared healthy.

These mice are the controls for MMRRC:44026.

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

David Russell, M.D., University of Washington Medical Center.