Strain Name:
C57BL/6J-MtgxR5835Btlr
Stock Number:
044056-MU
Citation ID:
RRID:MMRRC_044056-MU
Other Names:
R5835 (G1)
Major Collection:

Gene Information

Gene Symbol: Slc1a2 [MGI:101931] (Mus musculus (mouse))
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms: GLT-1, 2900019G14Rik, 1700091C19Rik, GLT1, MGLT1, Eaat2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Sfrp1 [MGI:892014] (Mus musculus (mouse))
Name: secreted frizzled-related protein 1
Synonyms: sFRP-1, 2210415K03Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Prrc2b [MGI:1923304] (Mus musculus (mouse))
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Rybp [MGI:1929059] (Mus musculus (mouse))
Name: RING1 and YY1 binding protein
Synonyms: DEDAF, 2410018J24Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Tex2 [MGI:102465] (Mus musculus (mouse))
Name: testis expressed gene 2
Synonyms: Taz4, 4930568E07Rik, Def-5
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Arfgef1 [MGI:2442988] (Mus musculus (mouse))
Name: ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
Synonyms: D130059B05Rik, ARFGEP1, D730028O18Rik, BIG1, P200
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Ccdc18 [MGI:1922974] (Mus musculus (mouse))
Name: coiled-coil domain containing 18
Synonyms: 1700021E15Rik, 4932411G06Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,160,739 bp
  • T to C, chromosome 1 at 20,201,083 bp
  • T to A, chromosome 2 at 32,206,473 bp
  • A to G, chromosome 2 at 102,777,450 bp
  • A to G, chromosome 2 at 121,337,899 bp
  • T to G, chromosome 2 at 122,327,860 bp
  • T to C, chromosome 3 at 63,697,522 bp
  • T to G, chromosome 3 at 121,527,200 bp
  • T to A, chromosome 4 at 11,514,036 bp
  • A to G, chromosome 4 at 148,565,387 bp
  • A to G, chromosome 5 at 34,813,190 bp
  • T to G, chromosome 5 at 97,056,982 bp
  • T to C, chromosome 5 at 108,140,874 bp
  • C to A, chromosome 5 at 137,456,655 bp
  • G to A, chromosome 6 at 41,118,840 bp
  • C to A, chromosome 6 at 42,267,586 bp
  • A to G, chromosome 6 at 69,992,528 bp
  • A to G, chromosome 6 at 100,233,190 bp
  • A to G, chromosome 6 at 121,639,336 bp
  • A to G, chromosome 7 at 10,527,820 bp
  • C to A, chromosome 7 at 28,881,958 bp
  • T to C, chromosome 7 at 35,529,264 bp
  • A to T, chromosome 7 at 43,636,958 bp
  • C to A, chromosome 8 at 3,945,699 bp
  • A to G, chromosome 8 at 23,446,244 bp
  • T to C, chromosome 8 at 94,392,239 bp
  • A to T, chromosome 9 at 16,375,833 bp
  • G to A, chromosome 9 at 35,561,184 bp
  • T to A, chromosome 10 at 43,975,133 bp
  • T to A, chromosome 10 at 82,151,212 bp
  • A to G, chromosome 10 at 129,659,934 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • T to C, chromosome 11 at 59,002,081 bp
  • C to T, chromosome 11 at 59,042,127 bp
  • A to T, chromosome 11 at 72,789,210 bp
  • A to G, chromosome 11 at 74,638,012 bp
  • C to T, chromosome 11 at 106,519,913 bp
  • A to T, chromosome 11 at 106,836,148 bp
  • T to C, chromosome 12 at 112,775,796 bp
  • A to G, chromosome 13 at 58,313,146 bp
  • T to C, chromosome 14 at 54,950,407 bp
  • A to G, chromosome 14 at 68,655,681 bp
  • A to G, chromosome 14 at 108,911,572 bp
  • A to T, chromosome 15 at 98,136,382 bp
  • T to G, chromosome 16 at 33,987,091 bp
  • A to T, chromosome 17 at 26,958,218 bp
  • A to T, chromosome 17 at 29,148,238 bp
  • G to A, chromosome 17 at 43,478,164 bp
  • T to C, chromosome 18 at 61,823,922 bp
  • C to A, chromosome 18 at 84,612,833 bp
  • T to A, chromosome 19 at 4,738,219 bp
  • A to G, chromosome 19 at 6,063,884 bp
  • T to A, chromosome 19 at 56,306,786 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5835 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044056-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.