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Strain Name:
C57BL/6J-MtgxR5835Btlr/Mmmh
Stock Number:
044056-MU
Citation ID:
RRID:MMRRC_044056-MU
Other Names:
R5835 (G1)
Major Collection:

Strain Information

Slc1a2
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms: GLT1, MGLT1, Eaat2, 1700091C19Rik, GLT-1, 2900019G14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20511
Homologene: 3075
Sfrp1
Name: secreted frizzled-related protein 1
Synonyms: sFRP-1, 2210415K03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20377
Homologene: 2266
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Rybp
Name: RING1 and YY1 binding protein
Synonyms: 2410018J24Rik, DEDAF
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56353
Homologene: 8159
Tex2
Name: testis expressed gene 2
Synonyms: Taz4, Def-5, 4930568E07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21763
Homologene: 32414
Arfgef1
Name: ARF guanine nucleotide exchange factor 1
Synonyms: D730028O18Rik, ARFGEP1, P200, BIG1, D130059B05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 211673
Homologene: 4687
Ccdc18
Name: coiled-coil domain containing 18
Synonyms: 1700021E15Rik, 4932411G06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 73254
Homologene: 35455
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,160,739 bp
  • T to C, chromosome 1 at 20,201,083 bp
  • T to A, chromosome 2 at 32,206,473 bp
  • A to G, chromosome 2 at 102,777,450 bp
  • A to G, chromosome 2 at 121,337,899 bp
  • T to G, chromosome 2 at 122,327,860 bp
  • T to C, chromosome 3 at 63,697,522 bp
  • T to G, chromosome 3 at 121,527,200 bp
  • T to A, chromosome 4 at 11,514,036 bp
  • A to G, chromosome 4 at 148,565,387 bp
  • A to G, chromosome 5 at 34,813,190 bp
  • T to G, chromosome 5 at 97,056,982 bp
  • T to C, chromosome 5 at 108,140,874 bp
  • C to A, chromosome 5 at 137,456,655 bp
  • G to A, chromosome 6 at 41,118,840 bp
  • C to A, chromosome 6 at 42,267,586 bp
  • A to G, chromosome 6 at 69,992,528 bp
  • A to G, chromosome 6 at 100,233,190 bp
  • A to G, chromosome 6 at 121,639,336 bp
  • A to G, chromosome 7 at 10,527,820 bp
  • C to A, chromosome 7 at 28,881,958 bp
  • T to C, chromosome 7 at 35,529,264 bp
  • A to T, chromosome 7 at 43,636,958 bp
  • C to A, chromosome 8 at 3,945,699 bp
  • A to G, chromosome 8 at 23,446,244 bp
  • T to C, chromosome 8 at 94,392,239 bp
  • A to T, chromosome 9 at 16,375,833 bp
  • G to A, chromosome 9 at 35,561,184 bp
  • T to A, chromosome 10 at 43,975,133 bp
  • T to A, chromosome 10 at 82,151,212 bp
  • A to G, chromosome 10 at 129,659,934 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • T to C, chromosome 11 at 59,002,081 bp
  • C to T, chromosome 11 at 59,042,127 bp
  • A to T, chromosome 11 at 72,789,210 bp
  • A to G, chromosome 11 at 74,638,012 bp
  • C to T, chromosome 11 at 106,519,913 bp
  • A to T, chromosome 11 at 106,836,148 bp
  • T to C, chromosome 12 at 112,775,796 bp
  • A to G, chromosome 13 at 58,313,146 bp
  • T to C, chromosome 14 at 54,950,407 bp
  • A to G, chromosome 14 at 68,655,681 bp
  • A to G, chromosome 14 at 108,911,572 bp
  • A to T, chromosome 15 at 98,136,382 bp
  • T to G, chromosome 16 at 33,987,091 bp
  • A to T, chromosome 17 at 26,958,218 bp
  • A to T, chromosome 17 at 29,148,238 bp
  • G to A, chromosome 17 at 43,478,164 bp
  • T to C, chromosome 18 at 61,823,922 bp
  • C to A, chromosome 18 at 84,612,833 bp
  • T to A, chromosome 19 at 4,738,219 bp
  • A to G, chromosome 19 at 6,063,884 bp
  • T to A, chromosome 19 at 56,306,786 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5835 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044056-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.