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Strain Name:
C57BL/6J-MtgxR5841Btlr/Mmmh
Stock Number:
044061-MU
Citation ID:
RRID:MMRRC_044061-MU
Other Names:
R5841 (G1)
Major Collection:

Strain Information

Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Rgs8
Name: regulator of G-protein signaling 8
Synonyms: 6530413N01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67792
Homologene: 23152
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Zmym6
Name: zinc finger, MYM-type 6
Synonyms: D4Wsu24e, 9330177P20Rik, Zfp258
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100177
Homologene: 5186
Cdyl
Name: chromodomain protein, Y chromosome-like
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12593
VEGA: 13
HGNC: HGNC:1811
Homologene: 3548
Ckap5
Name: cytoskeleton associated protein 5
Synonyms: 3110043H24Rik, 4930432B04Rik, D730027C18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75786
Homologene: 8844
Polr3a
Name: polymerase (RNA) III (DNA directed) polypeptide A
Synonyms: RPC155, RPC1, 9330175N20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218832
VEGA: 14
Homologene: 5124
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 45,912,349 bp
  • A to G, chromosome 1 at 63,058,840 bp
  • A to T, chromosome 1 at 75,174,350 bp
  • A to T, chromosome 1 at 152,821,518 bp
  • A to G, chromosome 1 at 153,692,828 bp
  • G to T, chromosome 1 at 189,657,444 bp
  • T to A, chromosome 2 at 69,480,153 bp
  • T to A, chromosome 2 at 91,600,682 bp
  • T to A, chromosome 2 at 116,058,664 bp
  • C to T, chromosome 2 at 120,411,539 bp
  • A to G, chromosome 2 at 121,365,877 bp
  • A to T, chromosome 3 at 18,097,506 bp
  • T to G, chromosome 3 at 37,319,521 bp
  • G to A, chromosome 3 at 87,922,985 bp
  • C to T, chromosome 4 at 4,062,728 bp
  • A to G, chromosome 4 at 111,433,470 bp
  • A to T, chromosome 4 at 115,326,702 bp
  • A to G, chromosome 4 at 127,100,670 bp
  • A to G, chromosome 4 at 127,195,400 bp
  • A to G, chromosome 4 at 143,655,539 bp
  • A to T, chromosome 5 at 65,379,324 bp
  • T to C, chromosome 5 at 144,993,956 bp
  • T to C, chromosome 6 at 14,718,984 bp
  • C to T, chromosome 6 at 35,512,290 bp
  • T to A, chromosome 6 at 57,137,802 bp
  • T to C, chromosome 6 at 149,044,755 bp
  • T to A, chromosome 7 at 3,822,899 bp
  • G to A, chromosome 7 at 19,201,163 bp
  • T to C, chromosome 7 at 103,097,954 bp
  • TTCCTC to TTC, chromosome 7 at 119,951,021 bp
  • T to C, chromosome 8 at 110,533,214 bp
  • G to A, chromosome 9 at 7,347,501 bp
  • T to C, chromosome 9 at 57,744,602 bp
  • A to G, chromosome 10 at 78,172,118 bp
  • A to G, chromosome 10 at 79,859,932 bp
  • A to T, chromosome 10 at 102,388,965 bp
  • T to A, chromosome 11 at 6,507,000 bp
  • T to C, chromosome 11 at 21,998,594 bp
  • A to T, chromosome 11 at 55,125,721 bp
  • T to A, chromosome 11 at 60,203,584 bp
  • T to C, chromosome 11 at 68,492,270 bp
  • T to A, chromosome 11 at 94,505,610 bp
  • C to A, chromosome 11 at 99,772,317 bp
  • G to A, chromosome 11 at 116,602,354 bp
  • A to G, chromosome 12 at 55,252,829 bp
  • T to A, chromosome 12 at 81,918,655 bp
  • T to C, chromosome 12 at 101,985,238 bp
  • T to C, chromosome 13 at 35,872,561 bp
  • C to A, chromosome 13 at 49,238,726 bp
  • T to C, chromosome 13 at 112,695,322 bp
  • T to A, chromosome 14 at 8,049,596 bp
  • G to A, chromosome 14 at 20,550,283 bp
  • A to T, chromosome 14 at 24,450,698 bp
  • C to T, chromosome 14 at 78,994,518 bp
  • A to G, chromosome 15 at 9,182,570 bp
  • C to T, chromosome 15 at 63,826,210 bp
  • T to C, chromosome 15 at 89,308,068 bp
  • G to A, chromosome 15 at 98,852,109 bp
  • A to G, chromosome 17 at 24,496,209 bp
  • A to T, chromosome 17 at 24,644,877 bp
  • A to G, chromosome 17 at 25,235,417 bp
  • G to A, chromosome 17 at 84,633,562 bp
  • A to G, chromosome 18 at 34,605,523 bp
  • G to A, chromosome 18 at 45,559,396 bp
  • T to C, chromosome 19 at 10,223,547 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5841 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044061-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.