Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5847Btlr/Mmmh
Stock Number:
044065-MU
Citation ID:
RRID:MMRRC_044065-MU
Other Names:
R5847 (G1)
Major Collection:

Strain Information

Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, 1700092A20Rik, ZFYVE10
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Cnot2
Name: CCR4-NOT transcription complex, subunit 2
Synonyms: 2600016M12Rik, 2810470K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72068
HGNC: HGNC:7878
Homologene: 40953
Wwp1
Name: WW domain containing E3 ubiquitin protein ligase 1
Synonyms: SDRP1, Tiul1, AIP5, 8030445B08Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107568
Homologene: 21385
Rheb
Name: Ras homolog enriched in brain
Synonyms: Rheb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19744
Homologene: 123916
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Ppp1r10
Name: protein phosphatase 1, regulatory subunit 10
Synonyms: 2610025H06Rik, D17Ertd808e, PNUTS
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 52040
HGNC: HGNC:9284
Homologene: 2033
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 20,374,736 bp
  • A to G, chromosome 1 at 162,639,367 bp
  • A to C, chromosome 2 at 86,890,332 bp
  • A to T, chromosome 2 at 130,284,028 bp
  • A to G, chromosome 3 at 127,680,074 bp
  • A to T, chromosome 4 at 19,662,174 bp
  • A to T, chromosome 4 at 126,273,978 bp
  • A to G, chromosome 4 at 142,032,035 bp
  • A to T, chromosome 5 at 24,544,166 bp
  • T to A, chromosome 5 at 24,807,069 bp
  • C to T, chromosome 5 at 37,404,724 bp
  • G to T, chromosome 5 at 92,627,480 bp
  • T to A, chromosome 5 at 122,116,845 bp
  • C to T, chromosome 5 at 143,288,669 bp
  • T to C, chromosome 6 at 40,684,055 bp
  • T to A, chromosome 6 at 48,933,478 bp
  • A to T, chromosome 6 at 70,216,972 bp
  • A to T, chromosome 6 at 76,973,837 bp
  • C to A, chromosome 6 at 119,992,408 bp
  • T to A, chromosome 6 at 126,865,558 bp
  • A to T, chromosome 7 at 4,344,574 bp
  • T to C, chromosome 7 at 33,112,814 bp
  • A to T, chromosome 7 at 105,069,857 bp
  • T to C, chromosome 7 at 122,762,309 bp
  • T to C, chromosome 8 at 69,611,652 bp
  • T to C, chromosome 8 at 91,304,985 bp
  • G to A, chromosome 9 at 51,824,976 bp
  • C to A, chromosome 9 at 90,209,724 bp
  • A to G, chromosome 9 at 104,003,037 bp
  • T to A, chromosome 10 at 14,426,777 bp
  • T to A, chromosome 10 at 58,603,179 bp
  • A to T, chromosome 10 at 63,076,414 bp
  • G to T, chromosome 10 at 78,445,038 bp
  • T to C, chromosome 10 at 116,527,946 bp
  • A to G, chromosome 11 at 4,482,925 bp
  • A to G, chromosome 11 at 35,867,326 bp
  • T to C, chromosome 11 at 50,923,216 bp
  • T to A, chromosome 11 at 58,990,593 bp
  • CAGTTGCTTAAA to CA, chromosome 11 at 66,095,240 bp
  • A to G, chromosome 12 at 40,621,251 bp
  • A to G, chromosome 13 at 47,101,601 bp
  • G to A, chromosome 13 at 56,636,605 bp
  • A to G, chromosome 14 at 26,665,631 bp
  • A to G, chromosome 14 at 52,286,784 bp
  • T to C, chromosome 15 at 10,537,596 bp
  • A to T, chromosome 16 at 19,530,326 bp
  • C to A, chromosome 17 at 5,924,621 bp
  • A to G, chromosome 17 at 14,199,188 bp
  • A to G, chromosome 17 at 35,926,847 bp
  • G to A, chromosome 18 at 24,018,038 bp
  • T to C, chromosome 18 at 78,030,055 bp
  • C to T, chromosome 19 at 40,011,732 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5847 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044065-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.