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Strain Name:
C57BL/6J-MtgxR5860Btlr/Mmmh
Stock Number:
044072-MU
Citation ID:
RRID:MMRRC_044072-MU
Other Names:
R5860 (G1)
Major Collection:

Strain Information

Adgre1
Name: adhesion G protein-coupled receptor E1
Synonyms: F4/80, DD7A5-7, TM7LN3, EGF-TM7, Ly71, Emr1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13733
VEGA: 17
HGNC: HGNC:3336
Homologene: 1493
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, D10Ertd17e, Cxxc6, BB001228
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52463
Homologene: 12735
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Tpgs1
Name: tubulin polyglutamylase complex subunit 1
Synonyms: Gtrgeo22, Gm16517
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110012
VEGA: 10
Homologene: 17525
Trim13
Name: tripartite motif-containing 13
Synonyms: 3110001L12Rik, LEU5, Rfp2, RNF77
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66597
VEGA: 14
HGNC: HGNC:9976
Homologene: 4234
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 23,815,319 bp
  • A to G, chromosome 1 at 34,485,580 bp
  • T to C, chromosome 1 at 65,103,368 bp
  • A to G, chromosome 1 at 75,136,487 bp
  • A to G, chromosome 1 at 134,185,171 bp
  • T to C, chromosome 1 at 155,332,122 bp
  • A to T, chromosome 1 at 170,841,775 bp
  • G to A, chromosome 1 at 170,841,776 bp
  • T to C, chromosome 2 at 60,236,843 bp
  • T to A, chromosome 2 at 90,757,482 bp
  • T to C, chromosome 2 at 91,045,514 bp
  • T to C, chromosome 2 at 92,024,054 bp
  • G to A, chromosome 2 at 109,991,151 bp
  • C to A, chromosome 2 at 110,597,624 bp
  • G to T, chromosome 2 at 121,164,037 bp
  • C to A, chromosome 2 at 151,224,305 bp
  • G to A, chromosome 2 at 166,644,684 bp
  • T to C, chromosome 2 at 180,592,492 bp
  • C to T, chromosome 3 at 54,735,042 bp
  • T to A, chromosome 3 at 88,342,907 bp
  • A to G, chromosome 3 at 97,724,188 bp
  • A to G, chromosome 4 at 32,580,268 bp
  • T to C, chromosome 4 at 118,211,289 bp
  • T to C, chromosome 5 at 27,500,228 bp
  • A to G, chromosome 5 at 118,228,803 bp
  • A to T, chromosome 5 at 120,791,950 bp
  • T to A, chromosome 6 at 40,880,407 bp
  • T to A, chromosome 6 at 83,003,814 bp
  • A to G, chromosome 6 at 125,643,090 bp
  • G to T, chromosome 6 at 125,679,265 bp
  • T to C, chromosome 6 at 128,541,061 bp
  • T to C, chromosome 6 at 132,980,701 bp
  • T to C, chromosome 7 at 47,189,351 bp
  • G to A, chromosome 7 at 99,346,364 bp
  • C to T, chromosome 7 at 105,772,035 bp
  • A to C, chromosome 7 at 114,791,148 bp
  • C to A, chromosome 8 at 25,666,091 bp
  • T to G, chromosome 8 at 41,076,266 bp
  • C to T, chromosome 8 at 45,051,129 bp
  • G to T, chromosome 8 at 107,064,154 bp
  • T to A, chromosome 9 at 39,484,471 bp
  • A to G, chromosome 9 at 105,392,961 bp
  • T to C, chromosome 9 at 106,155,707 bp
  • G to A, chromosome 9 at 110,084,577 bp
  • T to C, chromosome 9 at 118,558,106 bp
  • A to G, chromosome 10 at 62,812,620 bp
  • G to A, chromosome 10 at 79,669,711 bp
  • C to T, chromosome 11 at 34,256,562 bp
  • A to G, chromosome 11 at 49,459,736 bp
  • C to T, chromosome 12 at 85,040,886 bp
  • A to T, chromosome 12 at 108,187,207 bp
  • A to T, chromosome 13 at 8,973,160 bp
  • A to C, chromosome 13 at 35,858,083 bp
  • T to A, chromosome 14 at 7,931,135 bp
  • T to A, chromosome 14 at 29,986,285 bp
  • T to G, chromosome 14 at 61,604,739 bp
  • T to C, chromosome 15 at 39,086,685 bp
  • A to T, chromosome 15 at 76,648,191 bp
  • T to C, chromosome 15 at 76,656,415 bp
  • A to G, chromosome 16 at 32,428,796 bp
  • C to A, chromosome 16 at 56,155,359 bp
  • A to C, chromosome 16 at 59,565,269 bp
  • A to G, chromosome 16 at 85,798,544 bp
  • C to A, chromosome 17 at 34,064,185 bp
  • G to T, chromosome 17 at 34,582,418 bp
  • T to A, chromosome 17 at 57,445,034 bp
  • T to C, chromosome 18 at 20,095,024 bp
  • C to A, chromosome 18 at 74,276,697 bp
  • A to G, chromosome 19 at 29,599,845 bp
  • A to T, chromosome 19 at 39,536,826 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5860 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044072-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.