Strain Name:
C57BL/6J-MtgxR5916Btlr
Stock Number:
044113-MU
Citation ID:
RRID:MMRRC_044113-MU
Other Names:
R5916 (G1)
Major Collection:

Gene Information

Gene Symbol: Utrn [MGI:104631] (Mus musculus (mouse))
Name: utrophin
Synonyms: DRP, G-utrophin, Dmdl
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Ntrk2 [MGI:97384] (Mus musculus (mouse))
Name: neurotrophic tyrosine kinase, receptor, type 2
Synonyms: C030027L06Rik, Tkrb, trkB
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Colgalt2 [MGI:2138232] (Mus musculus (mouse))
Name: collagen beta(1-O)galactosyltransferase 2
Synonyms: Glt25d2
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Agt [MGI:87963] (Mus musculus (mouse))
Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Synonyms: Aogen, angiotensin precursor, Serpina8
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Mga [MGI:1352483] (Mus musculus (mouse))
Name: MAX gene associated
Synonyms: Mga, C130042M01Rik, D030062C11Rik, Mad5
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: U2af2 [MGI:98886] (Mus musculus (mouse))
Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Tbc1d22a [MGI:1289265] (Mus musculus (mouse))
Name: TBC1 domain family, member 22a
Synonyms: D15Ertd781e
Chromosome: 15
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 39,688,619 bp
  • C to T, chromosome 1 at 132,357,691 bp
  • T to A, chromosome 1 at 132,937,800 bp
  • T to A, chromosome 1 at 152,504,122 bp
  • A to T, chromosome 1 at 174,369,132 bp
  • T to G, chromosome 2 at 24,192,794 bp
  • T to A, chromosome 2 at 31,396,139 bp
  • A to T, chromosome 2 at 69,789,462 bp
  • A to T, chromosome 2 at 110,681,836 bp
  • A to G, chromosome 2 at 111,960,830 bp
  • T to A, chromosome 2 at 119,964,312 bp
  • G to T, chromosome 2 at 121,164,037 bp
  • G to T, chromosome 2 at 174,121,544 bp
  • T to C, chromosome 3 at 58,846,362 bp
  • T to C, chromosome 3 at 108,205,662 bp
  • A to T, chromosome 3 at 154,695,823 bp
  • T to C, chromosome 4 at 88,357,541 bp
  • A to T, chromosome 4 at 144,622,980 bp
  • A to G, chromosome 4 at 154,249,425 bp
  • T to A, chromosome 5 at 145,205,302 bp
  • G to A, chromosome 6 at 57,646,203 bp
  • T to A, chromosome 6 at 113,470,123 bp
  • C to T, chromosome 6 at 149,326,578 bp
  • A to T, chromosome 7 at 5,079,180 bp
  • G to A, chromosome 7 at 102,484,379 bp
  • A to T, chromosome 7 at 105,749,380 bp
  • C to A, chromosome 7 at 105,759,166 bp
  • A to G, chromosome 7 at 109,831,724 bp
  • A to T, chromosome 8 at 24,684,539 bp
  • G to T, chromosome 8 at 66,387,111 bp
  • T to A, chromosome 8 at 69,807,098 bp
  • T to C, chromosome 8 at 84,977,876 bp
  • C to A, chromosome 8 at 91,252,913 bp
  • A to T, chromosome 8 at 124,563,858 bp
  • A to T, chromosome 8 at 125,468,573 bp
  • A to G, chromosome 9 at 7,102,309 bp
  • T to C, chromosome 9 at 26,854,736 bp
  • T to A, chromosome 10 at 12,665,051 bp
  • C to A, chromosome 10 at 52,933,221 bp
  • C to T, chromosome 10 at 60,358,037 bp
  • G to A, chromosome 10 at 103,221,382 bp
  • A to T, chromosome 10 at 107,523,513 bp
  • A to G, chromosome 12 at 28,691,514 bp
  • C to T, chromosome 12 at 79,325,082 bp
  • G to T, chromosome 12 at 103,323,876 bp
  • T to C, chromosome 13 at 12,434,471 bp
  • A to G, chromosome 13 at 58,808,729 bp
  • T to C, chromosome 13 at 93,175,547 bp
  • A to C, chromosome 13 at 100,222,701 bp
  • T to G, chromosome 13 at 100,506,179 bp
  • T to A, chromosome 14 at 26,706,918 bp
  • G to A, chromosome 14 at 65,202,729 bp
  • T to C, chromosome 14 at 76,134,900 bp
  • A to T, chromosome 15 at 75,451,178 bp
  • A to G, chromosome 15 at 86,214,608 bp
  • A to T, chromosome 15 at 99,321,828 bp
  • G to T, chromosome 16 at 14,465,142 bp
  • T to A, chromosome 16 at 92,320,671 bp
  • T to C, chromosome 16 at 97,451,733 bp
  • C to T, chromosome 17 at 37,445,679 bp
  • A to G, chromosome 18 at 3,511,463 bp
  • T to C, chromosome 18 at 19,987,020 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5916 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044113-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.