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Strain Name:
C57BL/6J-MtgxR5933Btlr/Mmmh
Stock Number:
044127-MU
Citation ID:
RRID:MMRRC_044127-MU
Other Names:
R5933 (G1)
Major Collection:

Strain Information

Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, 1700092A20Rik, ZFYVE10
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14026
VEGA: 12
Homologene: 56752
Rasgrp2
Name: RAS, guanyl releasing protein 2
Synonyms: CalDAG-GEFI, Caldaggef1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19395
HGNC: HGNC:9879
Homologene: 4250
Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Tkfc
Name: triokinase, FMN cyclase
Synonyms: Dak
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225913
VEGA: 19
Homologene: 56710
Uaca
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: nucling, 2700059D02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72565
VEGA: 9
Homologene: 74297
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 46,519,215 bp
  • T to A, chromosome 1 at 59,704,665 bp
  • A to G, chromosome 1 at 74,485,982 bp
  • A to C, chromosome 1 at 104,984,671 bp
  • A to G, chromosome 1 at 119,687,723 bp
  • T to A, chromosome 1 at 135,970,603 bp
  • A to G, chromosome 1 at 172,232,274 bp
  • G to A, chromosome 2 at 17,404,187 bp
  • T to C, chromosome 2 at 26,352,497 bp
  • A to T, chromosome 2 at 30,350,389 bp
  • T to C, chromosome 2 at 34,684,291 bp
  • A to G, chromosome 2 at 76,721,437 bp
  • A to T, chromosome 2 at 128,877,498 bp
  • T to C, chromosome 2 at 130,141,256 bp
  • T to C, chromosome 3 at 38,951,375 bp
  • T to A, chromosome 3 at 65,948,342 bp
  • T to C, chromosome 3 at 82,094,807 bp
  • T to A, chromosome 3 at 122,275,568 bp
  • T to C, chromosome 3 at 135,261,628 bp
  • T to A, chromosome 3 at 135,393,995 bp
  • C to T, chromosome 3 at 138,070,348 bp
  • T to C, chromosome 3 at 141,871,367 bp
  • A to T, chromosome 3 at 142,723,260 bp
  • A to T, chromosome 4 at 63,494,708 bp
  • C to T, chromosome 4 at 106,760,130 bp
  • A to G, chromosome 4 at 138,871,204 bp
  • G to A, chromosome 4 at 140,917,641 bp
  • A to T, chromosome 4 at 148,241,313 bp
  • A to G, chromosome 5 at 5,640,183 bp
  • A to T, chromosome 5 at 30,073,359 bp
  • A to T, chromosome 5 at 30,345,529 bp
  • A to T, chromosome 5 at 34,465,680 bp
  • C to A, chromosome 5 at 88,008,014 bp
  • T to A, chromosome 5 at 107,403,765 bp
  • G to A, chromosome 5 at 115,127,449 bp
  • G to A, chromosome 5 at 123,158,752 bp
  • C to A, chromosome 5 at 143,715,515 bp
  • A to T, chromosome 5 at 147,893,890 bp
  • T to C, chromosome 5 at 150,390,800 bp
  • A to G, chromosome 5 at 150,989,483 bp
  • T to C, chromosome 6 at 29,441,106 bp
  • T to A, chromosome 6 at 38,017,014 bp
  • T to A, chromosome 6 at 68,302,981 bp
  • T to C, chromosome 6 at 69,716,569 bp
  • C to T, chromosome 6 at 118,612,580 bp
  • A to T, chromosome 7 at 6,961,745 bp
  • A to T, chromosome 7 at 44,845,337 bp
  • G to T, chromosome 7 at 65,302,852 bp
  • T to A, chromosome 7 at 85,737,850 bp
  • T to A, chromosome 7 at 103,683,473 bp
  • A to T, chromosome 7 at 130,784,281 bp
  • C to T, chromosome 7 at 142,376,579 bp
  • T to C, chromosome 8 at 8,690,047 bp
  • A to G, chromosome 8 at 47,066,004 bp
  • T to A, chromosome 9 at 60,840,956 bp
  • C to T, chromosome 9 at 65,509,696 bp
  • A to G, chromosome 10 at 62,269,994 bp
  • T to A, chromosome 10 at 84,374,802 bp
  • A to G, chromosome 10 at 88,091,149 bp
  • G to A, chromosome 10 at 112,215,446 bp
  • C to T, chromosome 11 at 4,498,951 bp
  • A to G, chromosome 11 at 9,249,658 bp
  • G to A, chromosome 11 at 35,629,751 bp
  • A to T, chromosome 11 at 55,284,051 bp
  • A to G, chromosome 11 at 58,998,505 bp
  • C to T, chromosome 11 at 69,803,680 bp
  • A to C, chromosome 11 at 72,030,282 bp
  • T to C, chromosome 11 at 74,083,126 bp
  • A to T, chromosome 11 at 83,003,335 bp
  • A to T, chromosome 11 at 83,279,462 bp
  • A to G, chromosome 11 at 104,637,979 bp
  • C to T, chromosome 11 at 118,185,759 bp
  • C to T, chromosome 12 at 31,333,983 bp
  • T to C, chromosome 12 at 108,683,257 bp
  • T to C, chromosome 12 at 112,680,621 bp
  • A to G, chromosome 13 at 107,744,107 bp
  • T to A, chromosome 14 at 37,089,462 bp
  • T to A, chromosome 15 at 55,571,327 bp
  • A to G, chromosome 15 at 81,916,634 bp
  • T to C, chromosome 16 at 17,406,330 bp
  • A to G, chromosome 16 at 32,753,052 bp
  • A to T, chromosome 17 at 33,138,609 bp
  • A to T, chromosome 17 at 50,287,753 bp
  • A to T, chromosome 17 at 73,526,305 bp
  • C to G, chromosome 17 at 74,599,237 bp
  • T to A, chromosome 17 at 74,599,238 bp
  • C to A, chromosome 17 at 80,337,280 bp
  • G to T, chromosome 18 at 61,698,473 bp
  • T to G, chromosome 19 at 6,402,513 bp
  • C to A, chromosome 19 at 10,597,347 bp
  • T to A, chromosome 19 at 11,705,389 bp
  • A to G, chromosome 19 at 11,939,772 bp
  • T to C, chromosome 19 at 16,660,530 bp
  • T to C, chromosome 19 at 18,704,105 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5933 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044127-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.