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Strain Name:
C57BL/6J-MtgxR5940Btlr/Mmmh
Stock Number:
044132-MU
Citation ID:
RRID:MMRRC_044132-MU
Other Names:
R5940 (G1)
Major Collection:

Strain Information

Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Nrg1
Name: neuregulin 1
Synonyms: HGL, HRG, Hgl, SMDF, GGF, ARIA, heregulin, D230005F13Rik, HRGalpha, 6030402G23Rik, GGFII, NDF
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 211323
HGNC: HGNC:7997
Homologene: 138451
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Aasdh
Name: aminoadipate-semialdehyde dehydrogenase
Synonyms: A230062G08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231326
Homologene: 71711
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208628
Homologene: 32227
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 43,737,241 bp
  • G to T, chromosome 1 at 88,351,415 bp
  • G to T, chromosome 1 at 92,917,712 bp
  • A to G, chromosome 1 at 150,657,222 bp
  • A to G, chromosome 1 at 171,417,779 bp
  • A to G, chromosome 2 at 79,887,839 bp
  • A to T, chromosome 2 at 86,658,050 bp
  • G to A, chromosome 2 at 111,292,384 bp
  • A to T, chromosome 2 at 148,442,232 bp
  • A to T, chromosome 2 at 155,415,865 bp
  • C to A, chromosome 3 at 27,115,465 bp
  • T to C, chromosome 3 at 38,889,649 bp
  • T to G, chromosome 3 at 87,975,952 bp
  • C to T, chromosome 3 at 88,984,036 bp
  • G to T, chromosome 3 at 98,711,971 bp
  • T to C, chromosome 3 at 125,561,419 bp
  • T to G, chromosome 3 at 127,670,946 bp
  • T to A, chromosome 3 at 129,312,578 bp
  • A to T, chromosome 4 at 9,449,443 bp
  • T to C, chromosome 4 at 83,476,322 bp
  • A to G, chromosome 4 at 107,893,288 bp
  • T to A, chromosome 4 at 123,432,881 bp
  • T to C, chromosome 4 at 145,074,975 bp
  • C to A, chromosome 4 at 152,283,375 bp
  • T to C, chromosome 5 at 24,722,662 bp
  • T to C, chromosome 5 at 35,652,980 bp
  • C to T, chromosome 5 at 51,473,911 bp
  • A to G, chromosome 5 at 65,643,985 bp
  • G to T, chromosome 5 at 76,882,898 bp
  • T to G, chromosome 5 at 123,786,195 bp
  • T to A, chromosome 5 at 135,064,559 bp
  • T to A, chromosome 6 at 31,489,372 bp
  • T to C, chromosome 6 at 90,370,325 bp
  • A to G, chromosome 6 at 125,168,869 bp
  • A to T, chromosome 6 at 129,674,935 bp
  • A to T, chromosome 6 at 136,613,721 bp
  • T to A, chromosome 7 at 48,871,077 bp
  • A to G, chromosome 7 at 79,454,071 bp
  • T to A, chromosome 7 at 96,845,895 bp
  • A to T, chromosome 7 at 131,238,263 bp
  • T to C, chromosome 8 at 31,849,344 bp
  • A to T, chromosome 8 at 45,321,784 bp
  • A to G, chromosome 8 at 71,890,220 bp
  • A to G, chromosome 8 at 106,210,805 bp
  • C to T, chromosome 9 at 37,919,437 bp
  • G to T, chromosome 9 at 38,697,711 bp
  • T to A, chromosome 9 at 92,591,397 bp
  • T to A, chromosome 9 at 101,101,091 bp
  • A to T, chromosome 9 at 108,091,899 bp
  • T to A, chromosome 10 at 59,456,732 bp
  • T to C, chromosome 10 at 69,920,486 bp
  • T to C, chromosome 10 at 78,405,415 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • T to C, chromosome 11 at 32,209,847 bp
  • T to A, chromosome 11 at 44,621,221 bp
  • C to T, chromosome 11 at 68,822,571 bp
  • T to C, chromosome 11 at 103,613,886 bp
  • C to T, chromosome 11 at 109,473,196 bp
  • A to G, chromosome 12 at 10,375,515 bp
  • T to A, chromosome 12 at 55,304,874 bp
  • C to T, chromosome 12 at 87,075,172 bp
  • A to G, chromosome 13 at 38,196,026 bp
  • T to G, chromosome 13 at 92,249,843 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • T to C, chromosome 14 at 32,662,688 bp
  • T to A, chromosome 14 at 51,070,722 bp
  • T to A, chromosome 14 at 57,035,860 bp
  • A to T, chromosome 14 at 63,000,910 bp
  • T to C, chromosome 14 at 73,029,491 bp
  • T to A, chromosome 14 at 73,029,949 bp
  • A to T, chromosome 16 at 3,865,821 bp
  • G to T, chromosome 16 at 20,734,586 bp
  • T to C, chromosome 16 at 94,134,712 bp
  • C to A, chromosome 17 at 23,670,341 bp
  • G to A, chromosome 17 at 35,524,993 bp
  • G to A, chromosome 17 at 57,210,244 bp
  • A to T, chromosome 18 at 78,755,488 bp
  • T to C, chromosome 19 at 5,423,000 bp
  • T to C, chromosome 19 at 11,192,966 bp
  • T to A, chromosome 19 at 13,619,153 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5940 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044132-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.