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Strain Name:
C57BL/6J-MtgxR5941Btlr/Mmmh
Stock Number:
044133-MU
Citation ID:
RRID:MMRRC_044133-MU
Other Names:
R5941 (G1)
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72895
Homologene: 12485
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Prss12
Name: serine protease 12 neurotrypsin (motopsin)
Synonyms: Bssp-3, motopsin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19142
HGNC: HGNC:9477
Homologene: 7490
Plekha7
Name: pleckstrin homology domain containing, family A member 7
Synonyms: A430081P20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233765
Homologene: 52172
Wee1
Name: WEE 1 homolog 1 (S. pombe)
Synonyms: Wee1A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22390
Homologene: 31152
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 36,770,949 bp
  • C to A, chromosome 1 at 46,187,290 bp
  • G to T, chromosome 2 at 37,561,896 bp
  • T to A, chromosome 2 at 89,054,619 bp
  • T to A, chromosome 2 at 89,844,515 bp
  • A to G, chromosome 2 at 114,197,546 bp
  • A to T, chromosome 2 at 120,713,558 bp
  • T to A, chromosome 2 at 122,344,156 bp
  • C to A, chromosome 2 at 127,428,275 bp
  • C to A, chromosome 2 at 128,127,783 bp
  • A to G, chromosome 2 at 129,075,984 bp
  • C to T, chromosome 2 at 178,481,650 bp
  • T to C, chromosome 3 at 37,320,048 bp
  • T to G, chromosome 3 at 88,376,346 bp
  • T to C, chromosome 3 at 105,732,069 bp
  • A to G, chromosome 3 at 123,505,501 bp
  • T to C, chromosome 3 at 152,766,513 bp
  • A to G, chromosome 4 at 18,054,354 bp
  • T to A, chromosome 4 at 33,162,676 bp
  • T to A, chromosome 4 at 65,314,593 bp
  • T to A, chromosome 4 at 115,876,637 bp
  • A to T, chromosome 5 at 20,774,073 bp
  • A to C, chromosome 5 at 120,609,561 bp
  • T to A, chromosome 6 at 18,313,646 bp
  • T to C, chromosome 6 at 35,232,408 bp
  • T to C, chromosome 6 at 41,048,401 bp
  • T to A, chromosome 6 at 41,059,629 bp
  • G to T, chromosome 6 at 42,955,716 bp
  • T to A, chromosome 6 at 113,128,490 bp
  • T to G, chromosome 6 at 135,736,373 bp
  • G to A, chromosome 7 at 30,292,306 bp
  • C to A, chromosome 7 at 64,745,716 bp
  • A to G, chromosome 7 at 79,711,132 bp
  • A to T, chromosome 7 at 81,078,653 bp
  • A to G, chromosome 7 at 103,492,720 bp
  • TCCCC to TCCC, chromosome 7 at 110,124,569 bp
  • T to C, chromosome 7 at 116,124,805 bp
  • T to A, chromosome 7 at 119,591,098 bp
  • A to G, chromosome 8 at 15,932,471 bp
  • A to G, chromosome 8 at 21,997,496 bp
  • G to T, chromosome 8 at 107,064,154 bp
  • A to G, chromosome 8 at 124,546,086 bp
  • A to G, chromosome 8 at 125,473,536 bp
  • A to G, chromosome 9 at 7,697,645 bp
  • A to C, chromosome 9 at 15,999,501 bp
  • A to C, chromosome 9 at 40,670,699 bp
  • A to T, chromosome 9 at 45,976,107 bp
  • A to G, chromosome 9 at 108,947,486 bp
  • A to G, chromosome 9 at 119,126,312 bp
  • T to C, chromosome 10 at 12,486,483 bp
  • C to T, chromosome 10 at 33,949,462 bp
  • C to T, chromosome 10 at 61,221,895 bp
  • G to T, chromosome 10 at 80,413,044 bp
  • T to A, chromosome 10 at 80,612,421 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • A to C, chromosome 11 at 67,259,300 bp
  • G to A, chromosome 11 at 69,879,973 bp
  • A to T, chromosome 11 at 74,392,237 bp
  • T to C, chromosome 11 at 80,251,170 bp
  • G to A, chromosome 11 at 80,331,889 bp
  • G to C, chromosome 11 at 86,250,972 bp
  • G to A, chromosome 12 at 104,946,992 bp
  • C to A, chromosome 12 at 109,743,299 bp
  • C to T, chromosome 12 at 112,113,085 bp
  • T to A, chromosome 12 at 117,728,738 bp
  • T to A, chromosome 13 at 11,687,902 bp
  • A to G, chromosome 13 at 18,031,202 bp
  • T to C, chromosome 13 at 69,739,340 bp
  • T to A, chromosome 13 at 89,692,691 bp
  • G to A, chromosome 13 at 93,498,825 bp
  • A to G, chromosome 13 at 94,440,273 bp
  • T to C, chromosome 13 at 94,483,265 bp
  • G to A, chromosome 14 at 18,586,910 bp
  • A to G, chromosome 14 at 26,706,867 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • C to A, chromosome 14 at 52,182,196 bp
  • C to T, chromosome 14 at 52,410,068 bp
  • T to A, chromosome 15 at 58,431,317 bp
  • A to T, chromosome 15 at 89,425,214 bp
  • A to C, chromosome 15 at 96,420,308 bp
  • T to C, chromosome 16 at 11,075,071 bp
  • A to G, chromosome 17 at 25,309,743 bp
  • C to T, chromosome 17 at 33,883,085 bp
  • G to A, chromosome 17 at 34,645,329 bp
  • A to G, chromosome 17 at 35,321,356 bp
  • G to A, chromosome 17 at 35,524,993 bp
  • T to C, chromosome 17 at 53,831,898 bp
  • G to A, chromosome 18 at 24,709,263 bp
  • G to A, chromosome 19 at 41,586,400 bp
  • T to C, chromosome 19 at 55,215,131 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5941 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044133-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.