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Strain Name:
C57BL/6J-MtgxR5950Btlr/Mmmh
Stock Number:
044140-MU
Citation ID:
RRID:MMRRC_044140-MU
Other Names:
R5950 (G1)
Major Collection:

Strain Information

Pitx2
Name: paired-like homeodomain transcription factor 2
Synonyms: Otlx2, Brx1b, Brx1a, Brx1, solurshin, Ptx2, Munc30, Pitx2c, Pitx2b, Pitx2a, Rieg
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18741
HGNC: HGNC:9005
Homologene: 55454
Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14026
VEGA: 12
Homologene: 56752
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Arid4b
Name: AT-rich interaction domain 4B
Synonyms: RBBP1L1, SAP180, BRCAA1, Rbp1l1, 6720480E17Rik, 6330417L24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94246
Homologene: 12847
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 34,262,060 bp
  • T to A, chromosome 1 at 62,216,531 bp
  • A to T, chromosome 1 at 69,683,244 bp
  • G to T, chromosome 1 at 74,377,176 bp
  • A to G, chromosome 1 at 74,855,402 bp
  • A to G, chromosome 1 at 87,021,108 bp
  • C to T, chromosome 1 at 170,834,879 bp
  • A to C, chromosome 2 at 18,306,941 bp
  • A to G, chromosome 2 at 61,653,569 bp
  • A to G, chromosome 2 at 67,511,320 bp
  • C to A, chromosome 2 at 128,997,790 bp
  • T to C, chromosome 3 at 64,565,231 bp
  • A to G, chromosome 3 at 129,218,520 bp
  • T to G, chromosome 4 at 44,931,244 bp
  • T to G, chromosome 4 at 103,004,250 bp
  • A to G, chromosome 4 at 112,019,335 bp
  • C to G, chromosome 4 at 123,439,436 bp
  • C to A, chromosome 5 at 25,517,743 bp
  • A to T, chromosome 5 at 108,445,485 bp
  • A to G, chromosome 5 at 147,024,350 bp
  • A to G, chromosome 7 at 3,694,788 bp
  • G to A, chromosome 7 at 10,490,571 bp
  • T to C, chromosome 7 at 62,419,719 bp
  • A to T, chromosome 7 at 79,773,431 bp
  • A to C, chromosome 7 at 118,853,334 bp
  • G to A, chromosome 7 at 120,382,656 bp
  • T to C, chromosome 8 at 93,362,959 bp
  • A to T, chromosome 8 at 104,488,485 bp
  • C to A, chromosome 8 at 125,941,760 bp
  • T to A, chromosome 9 at 24,581,134 bp
  • T to C, chromosome 9 at 39,462,337 bp
  • T to C, chromosome 9 at 96,839,126 bp
  • A to G, chromosome 9 at 119,811,124 bp
  • T to C, chromosome 10 at 8,248,101 bp
  • T to C, chromosome 10 at 29,143,648 bp
  • T to A, chromosome 10 at 39,030,448 bp
  • T to A, chromosome 10 at 76,488,419 bp
  • A to G, chromosome 10 at 86,701,745 bp
  • T to A, chromosome 11 at 60,187,593 bp
  • T to A, chromosome 11 at 70,609,586 bp
  • T to A, chromosome 12 at 55,738,265 bp
  • C to T, chromosome 12 at 108,675,553 bp
  • T to A, chromosome 13 at 14,191,264 bp
  • A to T, chromosome 13 at 54,091,287 bp
  • G to A, chromosome 13 at 64,787,769 bp
  • A to T, chromosome 13 at 67,044,817 bp
  • A to T, chromosome 14 at 8,255,793 bp
  • T to A, chromosome 14 at 20,665,935 bp
  • T to C, chromosome 14 at 54,144,311 bp
  • T to A, chromosome 14 at 96,240,354 bp
  • A to G, chromosome 15 at 44,532,965 bp
  • A to T, chromosome 15 at 76,715,310 bp
  • A to T, chromosome 15 at 86,032,500 bp
  • A to G, chromosome 16 at 31,665,583 bp
  • T to C, chromosome 16 at 44,479,847 bp
  • T to C, chromosome 16 at 59,493,571 bp
  • T to C, chromosome 16 at 96,372,872 bp
  • C to A, chromosome 17 at 28,202,558 bp
  • T to A, chromosome 17 at 28,905,755 bp
  • T to A, chromosome 17 at 32,977,507 bp
  • G to T, chromosome 17 at 49,715,088 bp
  • A to T, chromosome 17 at 75,273,870 bp
  • T to G, chromosome 17 at 84,740,170 bp
  • A to T, chromosome 18 at 20,538,529 bp
  • A to G, chromosome 18 at 64,510,485 bp
  • A to T, chromosome 19 at 3,602,333 bp
  • A to C, chromosome 19 at 5,667,158 bp
  • A to G, chromosome 19 at 36,597,239 bp
  • A to C, chromosome 19 at 44,152,090 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5950 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044140-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.