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Strain Name:
C57BL/6J-MtgxR5964Btlr/Mmmh
Stock Number:
044149-MU
Citation ID:
RRID:MMRRC_044149-MU
Other Names:
R5964 (G1)
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Casp8
Name: caspase 8
Synonyms: Mch5, MACH, FLICE, Caspase-8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12370
HGNC: HGNC:1509
Homologene: 7657
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 252870
Homologene: 2592
Ncoa7
Name: nuclear receptor coactivator 7
Synonyms: 9030406N13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 211329
VEGA: 10
Homologene: 65245
Prtg
Name: protogenin
Synonyms: A230098A12Rik, Igdcc5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235472
Homologene: 54453
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 39,624,954 bp
  • G to T, chromosome 1 at 58,833,736 bp
  • G to A, chromosome 1 at 66,725,922 bp
  • C to T, chromosome 1 at 87,407,167 bp
  • A to G, chromosome 1 at 131,978,073 bp
  • C to A, chromosome 1 at 139,455,227 bp
  • C to A, chromosome 2 at 76,713,511 bp
  • C to A, chromosome 2 at 76,829,888 bp
  • A to G, chromosome 2 at 130,636,288 bp
  • T to C, chromosome 2 at 139,678,757 bp
  • T to A, chromosome 2 at 180,031,425 bp
  • C to T, chromosome 3 at 10,434,481 bp
  • G to A, chromosome 3 at 89,343,567 bp
  • A to G, chromosome 3 at 116,793,774 bp
  • A to G, chromosome 3 at 145,576,933 bp
  • A to T, chromosome 3 at 146,680,696 bp
  • G to A, chromosome 4 at 21,683,852 bp
  • T to C, chromosome 4 at 126,128,895 bp
  • T to C, chromosome 5 at 20,775,934 bp
  • A to G, chromosome 5 at 67,417,818 bp
  • T to A, chromosome 5 at 136,023,699 bp
  • T to A, chromosome 5 at 145,788,467 bp
  • T to A, chromosome 6 at 124,326,572 bp
  • A to T, chromosome 7 at 18,900,940 bp
  • T to C, chromosome 7 at 25,926,223 bp
  • T to C, chromosome 7 at 72,103,177 bp
  • T to C, chromosome 7 at 80,261,933 bp
  • T to A, chromosome 7 at 81,092,260 bp
  • T to A, chromosome 7 at 119,449,129 bp
  • T to C, chromosome 7 at 119,922,880 bp
  • T to C, chromosome 7 at 121,956,029 bp
  • A to G, chromosome 7 at 128,140,447 bp
  • T to A, chromosome 8 at 14,727,128 bp
  • G to A, chromosome 8 at 93,966,999 bp
  • T to C, chromosome 8 at 94,068,367 bp
  • C to A, chromosome 9 at 21,647,430 bp
  • A to T, chromosome 9 at 71,868,240 bp
  • G to A, chromosome 9 at 72,892,254 bp
  • A to G, chromosome 9 at 75,203,833 bp
  • G to T, chromosome 9 at 116,110,255 bp
  • T to G, chromosome 9 at 123,779,434 bp
  • T to C, chromosome 10 at 30,704,636 bp
  • A to T, chromosome 10 at 80,382,587 bp
  • T to G, chromosome 10 at 85,882,886 bp
  • T to C, chromosome 11 at 4,135,898 bp
  • C to T, chromosome 11 at 35,700,236 bp
  • T to A, chromosome 11 at 71,123,020 bp
  • A to G, chromosome 11 at 103,542,120 bp
  • C to G, chromosome 13 at 15,726,162 bp
  • T to C, chromosome 13 at 46,771,524 bp
  • A to T, chromosome 13 at 55,321,576 bp
  • A to G, chromosome 14 at 24,164,089 bp
  • G to A, chromosome 14 at 30,957,079 bp
  • T to C, chromosome 14 at 33,106,011 bp
  • T to A, chromosome 14 at 46,767,217 bp
  • T to A, chromosome 14 at 50,534,198 bp
  • A to G, chromosome 15 at 28,458,584 bp
  • A to T, chromosome 15 at 34,410,165 bp
  • A to T, chromosome 16 at 4,000,951 bp
  • A to G, chromosome 16 at 8,712,102 bp
  • T to A, chromosome 17 at 6,065,049 bp
  • G to C, chromosome 17 at 33,336,845 bp
  • G to T, chromosome 17 at 56,271,703 bp
  • A to G, chromosome 17 at 80,644,762 bp
  • A to T, chromosome 18 at 89,207,183 bp
  • T to A, chromosome 19 at 3,365,760 bp
  • A to C, chromosome 19 at 12,954,531 bp
  • T to C, chromosome 19 at 27,420,051 bp
  • T to A, chromosome 19 at 34,648,469 bp
  • T to G, chromosome 19 at 37,699,962 bp
  • A to T, chromosome 19 at 43,469,723 bp
  • C to T, chromosome 19 at 46,654,180 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5964 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044149-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.