Strain Name:
C57BL/6J-MtgxR5971Btlr
Stock Number:
044154-MU
Citation ID:
RRID:MMRRC_044154-MU
Other Names:
R5971 (G1)
Major Collection:

Strain Information

Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Mgf, Sl, Gb, grizzle-belly, Steel, SCF, blz, Kitlg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Gcg
Name: glucagon
Synonyms: GLP-1, glucagon-like peptide I, Glu, PPG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14526
HGNC: HGNC:4191
Homologene: 1553
Ctnna1
Name: catenin (cadherin associated protein), alpha 1
Synonyms: alpha(E)-catenin, alpha E catenin, Catna1, 2010010M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433
Ppp4r1
Name: protein phosphatase 4, regulatory subunit 1
Synonyms: Pp4r1, 3110001J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70351
HGNC: HGNC:9320
Homologene: 81737
Polr1c
Name: polymerase (RNA) I polypeptide C
Synonyms: RPA40, 40kDa, Rpo1-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20016
Homologene: 3586
Lgr6
Name: leucine-rich repeat-containing G protein-coupled receptor 6
Synonyms: A530037C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329252
Homologene: 49680
Plagl1
Name: pleiomorphic adenoma gene-like 1
Synonyms: Zac1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22634
HGNC: HGNC:9046
Homologene: 31401
Abhd14a
Name: abhydrolase domain containing 14A
Synonyms: 1110013B16Rik, Dorz1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68644
Homologene: 22889
Abcc9
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 9
Synonyms: SUR2B, SUR2A, Sur2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20928
HGNC: HGNC:60
Homologene: 56521
Myo3b
Name: myosin IIIB
Synonyms: A430065P19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329421
Homologene: 51393
Adam39
Name: a disintegrin and metallopeptidase domain 39
Synonyms: 1700056P18Rik, testase 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 546055
HGNC: HGNC:199
Homologene: 128364
Theg
Name: testicular haploid expressed gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 21830
Homologene: 7976
Or1ak2
Name: olfactory receptor family 1 subfamily AK member 2
Synonyms: GA_x6K02T2NLDC-33631647-33632594, MOR134-1, Olfr356
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258617
Homologene: 85948
5031439G07Rik
Name: RIKEN cDNA 5031439G07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223739
HGNC: HGNC:1314
Homologene: 15140
Slc13a1
Name: solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms: NaSi-1, Nas1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 55961
Homologene: 31893
Pik3c2b
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
Synonyms: PI3K-C2beta, C330011J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240752
HGNC: HGNC:8972
Homologene: 20582
6820408C15Rik
Name: RIKEN cDNA 6820408C15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228778
Homologene: 51916
1700012B07Rik
Name: RIKEN cDNA 1700012B07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69324
Homologene: 78000
Anxa10
Name: annexin A10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26359
HGNC: HGNC:534
Homologene: 21409
Msl1
Name: male specific lethal 1
Synonyms: 4930463F05Rik, 2810017F12Rik, 4121402D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74026
Homologene: 129785
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 133,074,627 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • G to A, chromosome 2 at 36,937,229 bp
  • A to G, chromosome 2 at 62,475,804 bp
  • T to C, chromosome 2 at 70,238,899 bp
  • T to C, chromosome 2 at 152,440,870 bp
  • T to C, chromosome 6 at 24,133,657 bp
  • A to G, chromosome 6 at 142,639,575 bp
  • C to T, chromosome 8 at 40,824,593 bp
  • C to A, chromosome 8 at 62,077,926 bp
  • A to T, chromosome 9 at 106,443,866 bp
  • G to A, chromosome 10 at 13,127,746 bp
  • A to G, chromosome 10 at 79,584,755 bp
  • G to A, chromosome 10 at 100,076,906 bp
  • G to T, chromosome 11 at 98,798,693 bp
  • G to T, chromosome 11 at 109,794,154 bp
  • G to T, chromosome 15 at 84,987,662 bp
  • G to T, chromosome 17 at 46,247,709 bp
  • G to A, chromosome 17 at 65,814,348 bp
  • T to C, chromosome 18 at 35,154,514 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5971 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044154-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.