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Strain Name:
C57BL/6J-MtgxR5986Btlr/Mmmh
Stock Number:
044166-MU
Citation ID:
RRID:MMRRC_044166-MU
Other Names:
R5986 (G1)
Major Collection:

Strain Information

Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Ankib1
Name: ankyrin repeat and IBR domain containing 1
Synonyms: 2310061P20Rik, 4631416I11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70797
Homologene: 19076
Dpp3
Name: dipeptidylpeptidase 3
Synonyms: 4930533O14Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 75221
VEGA: 19
HGNC: HGNC:3008
Homologene: 40210
Ptbp3
Name: polypyrimidine tract binding protein 3
Synonyms: 5830471K22Rik, Rod1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230257
Homologene: 55872
Cog5
Name: component of oligomeric golgi complex 5
Synonyms: GOLTC1, GTC90, 5430405C01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238123
VEGA: 12
Homologene: 42221
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 133,272,307 bp
  • G to A, chromosome 2 at 150,408,024 bp
  • A to G, chromosome 2 at 155,979,277 bp
  • T to C, chromosome 3 at 103,085,397 bp
  • A to T, chromosome 3 at 116,772,496 bp
  • T to C, chromosome 3 at 127,012,686 bp
  • A to T, chromosome 3 at 152,740,849 bp
  • T to C, chromosome 4 at 59,493,311 bp
  • A to G, chromosome 4 at 62,455,876 bp
  • C to T, chromosome 4 at 113,995,648 bp
  • G to T, chromosome 4 at 144,332,753 bp
  • A to T, chromosome 4 at 153,965,593 bp
  • T to C, chromosome 5 at 3,747,071 bp
  • C to A, chromosome 5 at 22,746,426 bp
  • T to C, chromosome 5 at 30,588,378 bp
  • T to C, chromosome 5 at 103,038,580 bp
  • C to A, chromosome 6 at 103,709,191 bp
  • T to C, chromosome 7 at 16,782,226 bp
  • G to A, chromosome 7 at 20,844,522 bp
  • T to C, chromosome 7 at 99,876,255 bp
  • T to C, chromosome 7 at 103,187,528 bp
  • T to A, chromosome 7 at 133,720,865 bp
  • T to C, chromosome 8 at 64,074,263 bp
  • T to A, chromosome 9 at 15,998,317 bp
  • C to T, chromosome 9 at 20,675,650 bp
  • A to G, chromosome 9 at 39,144,982 bp
  • A to C, chromosome 9 at 56,259,442 bp
  • G to T, chromosome 9 at 107,551,822 bp
  • G to A, chromosome 9 at 110,490,026 bp
  • C to A, chromosome 9 at 120,461,421 bp
  • T to C, chromosome 10 at 52,404,072 bp
  • T to C, chromosome 10 at 117,759,176 bp
  • A to T, chromosome 10 at 127,304,302 bp
  • T to A, chromosome 11 at 74,792,237 bp
  • A to T, chromosome 12 at 31,660,717 bp
  • A to T, chromosome 12 at 69,185,384 bp
  • G to A, chromosome 12 at 84,308,346 bp
  • A to T, chromosome 13 at 36,734,264 bp
  • A to T, chromosome 13 at 50,474,460 bp
  • A to G, chromosome 13 at 118,384,565 bp
  • A to T, chromosome 14 at 49,232,946 bp
  • A to G, chromosome 14 at 93,887,048 bp
  • A to T, chromosome 14 at 119,049,426 bp
  • G to T, chromosome 15 at 6,844,453 bp
  • G to T, chromosome 15 at 27,851,933 bp
  • T to A, chromosome 15 at 59,654,602 bp
  • A to G, chromosome 15 at 75,045,608 bp
  • A to G, chromosome 16 at 35,314,091 bp
  • T to C, chromosome 16 at 89,789,186 bp
  • C to T, chromosome 17 at 20,951,346 bp
  • G to A, chromosome 17 at 25,367,134 bp
  • A to G, chromosome 17 at 30,851,630 bp
  • C to A, chromosome 17 at 32,544,142 bp
  • G to A, chromosome 17 at 55,962,030 bp
  • T to A, chromosome 18 at 12,905,081 bp
  • T to A, chromosome 18 at 77,254,782 bp
  • T to C, chromosome 19 at 4,918,357 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5986 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044166-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.