Strain Name:
C57BL/6J-MtgxR6025Btlr
Stock Number:
044197-MU
Citation ID:
RRID:MMRRC_044197-MU
Other Names:
R6025 (G1)
Major Collection:

Gene Information

Gene Symbol: Dct [MGI:102563] (Mus musculus (mouse))
Name: dopachrome tautomerase
Synonyms: tyrosinase-related protein-2, TRP-2, Tyrp2, Tyrp-2, TRP2
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Prkar2b [MGI:97760] (Mus musculus (mouse))
Name: protein kinase, cAMP dependent regulatory, type II beta
Synonyms: PKARIIbeta, RII(beta), Pkarb2
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Tns3 [MGI:2443012] (Mus musculus (mouse))
Name: tensin 3
Synonyms: TEM6, Tens1, F830010I22Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Slc2a1 [MGI:95755] (Mus musculus (mouse))
Name: solute carrier family 2 (facilitated glucose transporter), member 1
Synonyms: Glut-1, Glut1
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Lmnb1 [MGI:96795] (Mus musculus (mouse))
Name: lamin B1
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Tmem104 [MGI:2444222] (Mus musculus (mouse))
Name: transmembrane protein 104
Synonyms: C630005D06Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Dctn1 [MGI:107745] (Mus musculus (mouse))
Name: dynactin 1
Synonyms: p150, p150glued, Glued
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 66,695,568 bp
  • T to A, chromosome 1 at 90,828,102 bp
  • T to A, chromosome 1 at 173,595,234 bp
  • T to C, chromosome 2 at 60,375,962 bp
  • T to C, chromosome 2 at 91,114,340 bp
  • T to C, chromosome 2 at 91,945,910 bp
  • T to C, chromosome 2 at 122,301,851 bp
  • T to C, chromosome 2 at 160,965,582 bp
  • T to C, chromosome 2 at 174,426,818 bp
  • A to G, chromosome 3 at 146,007,141 bp
  • T to C, chromosome 4 at 15,981,347 bp
  • G to A, chromosome 4 at 33,181,833 bp
  • G to A, chromosome 4 at 42,938,780 bp
  • G to A, chromosome 4 at 116,950,909 bp
  • A to T, chromosome 4 at 119,136,342 bp
  • A to T, chromosome 5 at 4,032,801 bp
  • CATA to CA, chromosome 5 at 150,541,575 bp
  • A to G, chromosome 6 at 5,289,057 bp
  • T to C, chromosome 6 at 48,486,786 bp
  • T to A, chromosome 6 at 83,193,691 bp
  • G to A, chromosome 6 at 122,713,391 bp
  • A to T, chromosome 6 at 124,431,664 bp
  • G to A, chromosome 7 at 3,639,669 bp
  • T to A, chromosome 7 at 4,758,144 bp
  • A to G, chromosome 7 at 29,829,545 bp
  • G to T, chromosome 7 at 67,734,938 bp
  • T to A, chromosome 7 at 79,704,640 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • T to C, chromosome 7 at 103,768,209 bp
  • T to A, chromosome 7 at 143,106,433 bp
  • T to C, chromosome 8 at 27,114,463 bp
  • A to T, chromosome 8 at 71,712,573 bp
  • G to T, chromosome 8 at 86,713,373 bp
  • A to T, chromosome 9 at 15,862,797 bp
  • A to G, chromosome 9 at 21,115,960 bp
  • A to G, chromosome 9 at 108,360,123 bp
  • T to A, chromosome 10 at 9,800,028 bp
  • T to C, chromosome 10 at 53,615,977 bp
  • T to A, chromosome 10 at 80,710,464 bp
  • A to T, chromosome 11 at 8,492,578 bp
  • A to T, chromosome 11 at 22,239,156 bp
  • A to C, chromosome 11 at 22,518,659 bp
  • GC to G, chromosome 11 at 59,210,313 bp
  • A to G, chromosome 11 at 68,492,318 bp
  • A to T, chromosome 11 at 73,847,919 bp
  • G to A, chromosome 11 at 105,867,717 bp
  • T to C, chromosome 11 at 105,896,547 bp
  • C to A, chromosome 11 at 115,205,523 bp
  • C to T, chromosome 11 at 119,374,828 bp
  • A to G, chromosome 12 at 32,060,856 bp
  • C to A, chromosome 13 at 30,224,526 bp
  • C to A, chromosome 14 at 14,865,633 bp
  • T to C, chromosome 14 at 55,065,208 bp
  • T to C, chromosome 14 at 118,036,464 bp
  • C to A, chromosome 15 at 101,194,975 bp
  • T to C, chromosome 16 at 31,945,710 bp
  • T to G, chromosome 17 at 37,805,421 bp
  • C to T, chromosome 17 at 55,792,013 bp
  • T to A, chromosome 18 at 56,729,384 bp
  • A to T, chromosome 18 at 78,859,240 bp
  • G to A, chromosome 19 at 6,956,179 bp
  • T to A, chromosome 19 at 12,901,670 bp
  • A to T, chromosome 19 at 45,155,974 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6025 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044197-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.