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Strain Name:
C57BL/6J-MtgxR6032Btlr/Mmmh
Stock Number:
044204-MU
Citation ID:
RRID:MMRRC_044204-MU
Other Names:
R6032 (G1)
Major Collection:

Strain Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Dnajc3
Name: DnaJ heat shock protein family (Hsp40) member C3
Synonyms: p58IPK, mp58, Prkri, Dnajc3, Dnajc3a, Dnajc3b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100037258
VEGA: 14
HGNC: HGNC:9439
Homologene: 2486
Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: N-CoR, Rxrip13, A230020K14Rik, 5730405M06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
Wsb1
Name: WD repeat and SOCS box-containing 1
Synonyms: 2700038M07Rik, 1110056B13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78889
Homologene: 9218
Kcns2
Name: K+ voltage-gated channel, subfamily S, 2
Synonyms: Kv9.2, E130006J24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16539
VEGA: 15
HGNC: HGNC:6301
Homologene: 22465
Nrxn2
Name: neurexin II
Synonyms: neurexin II beta, neurexin II alpha, 6430591O13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 2 at 13,325,184 bp
  • T to C, chromosome 2 at 111,483,850 bp
  • T to C, chromosome 2 at 120,535,393 bp
  • A to T, chromosome 3 at 54,376,716 bp
  • T to C, chromosome 3 at 99,352,517 bp
  • A to G, chromosome 4 at 133,578,306 bp
  • A to G, chromosome 4 at 135,705,626 bp
  • A to C, chromosome 4 at 144,393,028 bp
  • A to T, chromosome 5 at 24,379,811 bp
  • G to A, chromosome 5 at 45,582,896 bp
  • A to G, chromosome 5 at 92,230,524 bp
  • A to C, chromosome 5 at 107,597,795 bp
  • G to T, chromosome 5 at 145,777,946 bp
  • T to A, chromosome 6 at 50,245,954 bp
  • T to A, chromosome 6 at 128,549,836 bp
  • T to C, chromosome 7 at 28,316,175 bp
  • A to G, chromosome 7 at 44,977,102 bp
  • C to T, chromosome 7 at 127,761,265 bp
  • T to C, chromosome 8 at 25,777,493 bp
  • C to T, chromosome 9 at 31,150,944 bp
  • A to G, chromosome 9 at 39,018,965 bp
  • A to T, chromosome 10 at 10,719,805 bp
  • T to C, chromosome 10 at 21,389,071 bp
  • A to G, chromosome 10 at 43,956,760 bp
  • C to T, chromosome 10 at 50,842,183 bp
  • G to T, chromosome 11 at 9,297,752 bp
  • A to G, chromosome 11 at 23,742,684 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • G to C, chromosome 11 at 55,253,934 bp
  • A to T, chromosome 11 at 62,373,321 bp
  • T to C, chromosome 11 at 79,240,199 bp
  • G to A, chromosome 11 at 101,325,801 bp
  • A to T, chromosome 11 at 120,494,958 bp
  • A to G, chromosome 12 at 7,995,513 bp
  • G to T, chromosome 13 at 21,555,907 bp
  • A to T, chromosome 13 at 83,662,359 bp
  • T to C, chromosome 14 at 68,579,184 bp
  • T to C, chromosome 14 at 118,968,031 bp
  • T to C, chromosome 15 at 34,838,934 bp
  • T to C, chromosome 15 at 72,925,530 bp
  • T to A, chromosome 15 at 75,311,599 bp
  • C to T, chromosome 15 at 76,658,858 bp
  • A to T, chromosome 15 at 85,066,229 bp
  • A to T, chromosome 16 at 3,980,157 bp
  • T to C, chromosome 16 at 11,984,908 bp
  • T to C, chromosome 16 at 44,106,940 bp
  • A to G, chromosome 16 at 96,649,991 bp
  • A to T, chromosome 17 at 36,871,714 bp
  • A to C, chromosome 17 at 49,486,497 bp
  • A to G, chromosome 17 at 67,750,643 bp
  • C to T, chromosome 18 at 3,267,673 bp
  • G to A, chromosome 18 at 77,381,558 bp
  • A to T, chromosome 19 at 6,517,132 bp
  • A to G, chromosome 19 at 12,429,383 bp
  • T to C, chromosome 19 at 14,452,108 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6032 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044204-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.