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Strain Name:
C57BL/6J-MtgxR6045Btlr/Mmmh
Stock Number:
044213-MU
Citation ID:
RRID:MMRRC_044213-MU
Other Names:
R6045 (G1)
Major Collection:

Strain Information

Galnt10
Name: polypeptide N-acetylgalactosaminyltransferase 10
Synonyms: GalNAc-T10, Galnt9, C330012K04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 171212
Homologene: 14924
Poli
Name: polymerase (DNA directed), iota
Synonyms: Rad30b
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 26447
HGNC: HGNC:9182
Homologene: 5209
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Cyp39a1
Name: cytochrome P450, family 39, subfamily a, polypeptide 1
Synonyms: oxysterol 7-alpha-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56050
VEGA: 17
Homologene: 9580
Morc3
Name: microrchidia 3
Synonyms: 1110051N18Rik, D16Jhu32e, 1110051N18Rik, Zcwcc3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 338467
Homologene: 32257
Gmps
Name: guanine monophosphate synthetase
Synonyms: Gm9479
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229363
HGNC: HGNC:4378
Homologene: 68367
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 44,119,073 bp
  • T to A, chromosome 1 at 139,424,032 bp
  • T to C, chromosome 1 at 160,645,323 bp
  • C to T, chromosome 1 at 173,216,500 bp
  • A to G, chromosome 2 at 40,701,813 bp
  • A to C, chromosome 2 at 52,194,425 bp
  • T to G, chromosome 2 at 112,130,536 bp
  • C to A, chromosome 2 at 118,388,815 bp
  • A to G, chromosome 2 at 131,856,022 bp
  • A to G, chromosome 2 at 181,240,313 bp
  • T to A, chromosome 3 at 5,396,959 bp
  • C to T, chromosome 3 at 63,980,137 bp
  • T to A, chromosome 3 at 90,613,879 bp
  • A to T, chromosome 3 at 103,746,902 bp
  • T to A, chromosome 4 at 63,344,692 bp
  • G to A, chromosome 4 at 118,484,691 bp
  • A to T, chromosome 4 at 155,386,474 bp
  • T to G, chromosome 5 at 14,937,181 bp
  • T to A, chromosome 5 at 38,234,008 bp
  • A to G, chromosome 5 at 65,279,549 bp
  • A to G, chromosome 5 at 73,118,551 bp
  • A to T, chromosome 5 at 90,584,989 bp
  • A to T, chromosome 5 at 105,218,403 bp
  • A to T, chromosome 5 at 120,631,712 bp
  • A to T, chromosome 6 at 13,219,936 bp
  • A to G, chromosome 6 at 29,379,870 bp
  • A to G, chromosome 6 at 49,252,464 bp
  • C to T, chromosome 6 at 72,568,868 bp
  • T to A, chromosome 6 at 84,114,072 bp
  • A to T, chromosome 6 at 87,085,257 bp
  • T to A, chromosome 6 at 121,321,628 bp
  • G to A, chromosome 6 at 136,821,535 bp
  • T to A, chromosome 6 at 145,621,174 bp
  • A to C, chromosome 7 at 16,996,031 bp
  • A to T, chromosome 7 at 18,856,564 bp
  • A to T, chromosome 7 at 45,425,009 bp
  • C to T, chromosome 7 at 104,564,767 bp
  • C to T, chromosome 7 at 119,476,823 bp
  • C to T, chromosome 7 at 119,967,522 bp
  • T to C, chromosome 7 at 127,031,078 bp
  • A to T, chromosome 8 at 21,095,232 bp
  • T to C, chromosome 8 at 79,229,367 bp
  • A to C, chromosome 8 at 105,844,187 bp
  • C to T, chromosome 9 at 26,883,209 bp
  • T to C, chromosome 9 at 70,419,093 bp
  • T to C, chromosome 9 at 105,925,918 bp
  • T to C, chromosome 9 at 114,437,942 bp
  • T to G, chromosome 9 at 123,371,988 bp
  • T to G, chromosome 10 at 19,609,161 bp
  • T to C, chromosome 10 at 25,993,007 bp
  • G to A, chromosome 10 at 70,957,081 bp
  • G to A, chromosome 10 at 92,695,100 bp
  • A to T, chromosome 10 at 127,566,600 bp
  • A to T, chromosome 11 at 33,624,008 bp
  • A to G, chromosome 11 at 57,783,793 bp
  • A to G, chromosome 11 at 67,244,724 bp
  • A to C, chromosome 11 at 69,352,118 bp
  • A to G, chromosome 11 at 69,651,051 bp
  • A to G, chromosome 11 at 81,893,868 bp
  • G to T, chromosome 11 at 102,059,354 bp
  • G to T, chromosome 12 at 17,348,478 bp
  • T to C, chromosome 14 at 47,676,796 bp
  • C to A, chromosome 14 at 54,477,768 bp
  • T to A, chromosome 14 at 73,568,964 bp
  • T to C, chromosome 14 at 103,592,213 bp
  • A to G, chromosome 15 at 35,671,316 bp
  • A to T, chromosome 16 at 20,711,688 bp
  • G to T, chromosome 16 at 37,856,022 bp
  • T to A, chromosome 16 at 59,186,091 bp
  • T to C, chromosome 16 at 85,899,300 bp
  • A to G, chromosome 16 at 93,874,845 bp
  • G to A, chromosome 17 at 18,168,043 bp
  • G to T, chromosome 17 at 43,731,991 bp
  • T to C, chromosome 17 at 70,818,098 bp
  • T to G, chromosome 17 at 74,446,959 bp
  • C to A, chromosome 17 at 86,809,399 bp
  • T to A, chromosome 18 at 10,547,068 bp
  • T to A, chromosome 18 at 37,321,575 bp
  • C to T, chromosome 18 at 70,517,469 bp
  • C to A, chromosome 19 at 12,210,295 bp
  • C to T, chromosome 19 at 33,966,844 bp
  • T to A, chromosome 19 at 50,190,117 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6045 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044213-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.