Strain Name:
C57BL/6J-MtgxR6046Btlr
Stock Number:
044214-MU
Citation ID:
RRID:MMRRC_044214-MU
Other Names:
R6046 (G1)
Major Collection:

Gene Information

Rgs9
Name: regulator of G-protein signaling 9
Synonyms: Rgs9-2, RGS9-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 19739
Homologene: 2845
Crhr2
Name: corticotropin releasing hormone receptor 2
Synonyms: Crfr2, CRF-R2, CRFR2beta, CRFR2alpha, CRF 2 receptor, CRH-R2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12922
HGNC: HGNC:2358
Homologene: 55612
Lrrn1
Name: leucine rich repeat protein 1, neuronal
Synonyms: NLRR-1, 2810047E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16979
Homologene: 32036
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Btbd11
Name: BTB (POZ) domain containing 11
Synonyms: 6330404E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74007
Homologene: 72536
Zfat
Name: zinc finger and AT hook domain containing
Synonyms: LOC380993, Zfp406, Zfat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 380993
Homologene: 16829
Rtn4
Name: reticulon 4
Synonyms: 1110020G17Rik, C130026I10Rik, NOGO, NgA, Nogo-B, Nogo-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68585
Homologene: 10743
Mettl3
Name: methyltransferase like 3
Synonyms: 2310024F18Rik, Spo8, M6A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56335
Homologene: 10501
Dop1a
Name: DOP1 leucine zipper like protein A
Synonyms: B130005I07Rik, D9Ertd809e, Dopey1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320615
Homologene: 26645
Sart3
Name: squamous cell carcinoma antigen recognized by T cells 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53890
Homologene: 40977
Hspa5
Name: heat shock protein 5
Synonyms: Hsce70, Bip, Grp78, Sez7, D2Wsu141e, D2Wsu17e, 78kDa, mBiP, XAP-1 antigen, baffled
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14828
HGNC: HGNC:5238
Homologene: 3908
Bnip3
Name: BCL2/adenovirus E1B interacting protein 3
Synonyms: Nip3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12176
HGNC: HGNC:1084
Homologene: 2990
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Sfxn1
Name: sideroflexin 1
Synonyms: 2810002O05Rik, A930015P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14057
VEGA: 13
Homologene: 41494
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Zfp866
Name: zinc finger protein 866
Synonyms: 9830167H18Rik, D330038O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330788
Zfp617
Name: zinc finger protein 617
Synonyms: Zfps11-6, Zinc finger protein s11-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170938
Homologene: 135689
Wnt2b
Name: wingless-type MMTV integration site family, member 2B
Synonyms: Wnt13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 22414
Homologene: 22526
Zfp991
Name: zinc finger protein 991
Synonyms: Gm13139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666532
Homologene: 133076
Glmp
Name: glycosylated lysosomal membrane protein
Synonyms: NCU-G1, 0610031J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56700
Homologene: 10562
Muc15
Name: mucin 15
Synonyms: D730046L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269328
Homologene: 51680
Arhgap44
Name: Rho GTPase activating protein 44
Synonyms: AU040829
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216831
Homologene: 65284
Wasf3
Name: WASP family, member 3
Synonyms: Wave3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 245880
Homologene: 68527
Sema4a
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
Synonyms: SemB, SemB, Semab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20351
Homologene: 8425
Lhx8
Name: LIM homeobox protein 8
Synonyms: L3, Lhx7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16875
Homologene: 62661
Ank1
Name: ankyrin 1, erythroid
Synonyms: Ank-1, pale
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11733
HGNC: HGNC:492
Homologene: 55427
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26875
Homologene: 69111
Col28a1
Name: collagen, type XXVIII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213945
Homologene: 66345
Pik3c2g
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18705
HGNC: HGNC:8973
Homologene: 3362
Calcrl
Name: calcitonin receptor-like
Synonyms: CRLR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 54598
Homologene: 21179
Btnl6
Name: butyrophilin-like 6
Synonyms: NG13, Gm6519
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 624681
Homologene: 106849
Dpyd
Name: dihydropyrimidine dehydrogenase
Synonyms: DPD, E330028L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99586
HGNC: HGNC:3012
Homologene: 85
Olfm2
Name: olfactomedin 2
Synonyms: A030009A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244723
VEGA: 9
Homologene: 14380
Unc13c
Name: unc-13 homolog C
Synonyms: Munc13-3, Unc13h3, D9Ertd414e, 1500037O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208898
Homologene: 45443
Zfp282
Name: zinc finger protein 282
Synonyms: HUB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101095
Homologene: 2647
Slc24a2
Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 2
Synonyms: 6330417K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76376
Homologene: 10669
Hfm1
Name: HFM1, ATP-dependent DNA helicase homolog
Synonyms: LOC381663, A330009G12Rik, Sec63d1, Mer3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330149
Homologene: 87103
Mroh2b
Name: maestro heat-like repeat family member 2B
Synonyms: 4930455B06Rik, Heatr7b2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223825
VEGA: 15
Homologene: 128807
Scn5a
Name: sodium channel, voltage-gated, type V, alpha
Synonyms: Nav1.5c, Nav1.5, mH1, SkM2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20271
Homologene: 22738
Ctsq
Name: cathepsin Q
Synonyms: 1600010J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 104002
Homologene: 122226
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: 4632428N09Rik, Aof1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218214
Homologene: 14695
Vmn2r25
Name: vomeronasal 2, receptor 25
Synonyms: EG545874
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 545874
Homologene: 135915
Rnpepl1
Name: arginyl aminopeptidase (aminopeptidase B)-like 1
Synonyms: 1110014H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108657
Homologene: 36367
Crybg2
Name: crystallin beta-gamma domain containing 2
Synonyms: Aim1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230806
Homologene: 19232
Trim75
Name: tripartite motif-containing 75
Synonyms: LOC333307
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 333307
Homologene: 53452
Ift140
Name: intraflagellar transport 140
Synonyms: Wdtc2, Tce5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106633
Homologene: 40979
Gm973
Name: predicted gene 973
Synonyms: LOC381260
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381260
Homologene: 124277
Celsr3
Name: cadherin, EGF LAG seven-pass G-type receptor 3
Synonyms: Fmi1, flamingo, Adgrc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 107934
HGNC: HGNC:3230
Homologene: 1077
Atp9a
Name: ATPase, class II, type 9A
Synonyms: Class II, IIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11981
Homologene: 69194
Qrich2
Name: glutamine rich 2
Synonyms: LOC217341
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217341
Homologene: 12951
Gbp3
Name: guanylate binding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 55932
Homologene: 137329
Wdhd1
Name: WD repeat and HMG-box DNA binding protein 1
Synonyms: AND-1, D630024B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218973
Homologene: 56019
Slc26a7
Name: solute carrier family 26, member 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 208890
Homologene: 13770
Psg28
Name: pregnancy-specific glycoprotein 28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114871
Homologene: 110989
Cc2d1a
Name: coiled-coil and C2 domain containing 1A
Synonyms: Freud-1, Tape
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 212139
Homologene: 23040
Olfr474
Name: olfactory receptor 474
Synonyms: GA_x6K02T2PBJ9-10283869-10284801, MOR204-20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258488
Irak1bp1
Name: interleukin-1 receptor-associated kinase 1 binding protein 1
Synonyms: AIP70, SIMPL, 4921528N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 65099
Homologene: 11300
Cfb
Name: complement factor B
Synonyms: B, Factor B, alternative-complement pathway C3/C5 convertase, Bf, FB, H2-Bf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14962
HGNC: HGNC:1037
Homologene: 1292
Mixl1
Name: Mix1 homeobox-like 1 (Xenopus laevis)
Synonyms: Mml
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 27217
Homologene: 8445
Ift27
Name: intraflagellar transport 27
Synonyms: 2600013G09Rik, Rabl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67042
VEGA: 15
Homologene: 4998
Itga3
Name: integrin alpha 3
Synonyms: VLA-3 alpha 3, alpha3-integrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16400
HGNC: HGNC:6139
Homologene: 21129
Cxcr1
Name: chemokine (C-X-C motif) receptor 1
Synonyms: Il8ra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227288
HGNC: HGNC:6026
Homologene: 68074
Tll1
Name: tolloid-like
Synonyms: Tll-1, b2b2476Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 21892
Homologene: 49202
Hspa14
Name: heat shock protein 14
Synonyms: NST-1, 70kDa, HSP70L1, Hsp70-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 50497
Homologene: 74307
Kctd15
Name: potassium channel tetramerisation domain containing 15
Synonyms: MGC25497
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233107
Homologene: 11450
Heatr3
Name: HEAT repeat containing 3
Synonyms: C030036P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234549
Homologene: 45429
Acyp2
Name: acylphosphatase 2, muscle type
Synonyms: 2310004B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75572
HGNC: HGNC:180
Homologene: 41776
Tbc1d10c
Name: TBC1 domain family, member 10c
Synonyms: 1810062O14Rik, Carabin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 108995
VEGA: 19
Homologene: 77854
Olfr622
Name: olfactory receptor 622
Synonyms: GA_x6K02T2PBJ9-6362863-6361910, MOR26-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259087
Homologene: 100540
Gm10113
Name: predicted gene 10113
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Smok2a
Name: sperm motility kinase 2A
Synonyms: Smok2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 27263
Homologene: 128738
Gm6486
Name: predicted gene 6486
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Olfr1468-ps1
Name: olfactory receptor 1468, pseudogene 1
Synonyms: GA_x6K02T2RE5P-3706029-3706953, MOR202-21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258192
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 59,632,350 bp
  • T to C, chromosome 1 at 74,192,281 bp
  • A to G, chromosome 1 at 92,916,821 bp
  • G to T, chromosome 1 at 180,696,771 bp
  • A to G, chromosome 2 at 3,489,764 bp
  • C to A, chromosome 2 at 34,775,749 bp
  • T to A, chromosome 2 at 69,506,754 bp
  • A to G, chromosome 2 at 84,375,314 bp
  • T to A, chromosome 2 at 110,731,441 bp
  • C to A, chromosome 2 at 168,634,870 bp
  • A to G, chromosome 3 at 88,325,188 bp
  • T to C, chromosome 3 at 88,440,701 bp
  • T to A, chromosome 3 at 104,951,023 bp
  • T to A, chromosome 3 at 119,431,575 bp
  • A to C, chromosome 3 at 142,567,799 bp
  • A to T, chromosome 3 at 154,321,703 bp
  • A to G, chromosome 4 at 14,505,471 bp
  • A to G, chromosome 4 at 86,996,645 bp
  • A to T, chromosome 4 at 134,092,077 bp
  • G to A, chromosome 4 at 147,179,765 bp
  • T to A, chromosome 5 at 3,070,846 bp
  • A to T, chromosome 5 at 14,713,288 bp
  • A to T, chromosome 5 at 106,898,643 bp
  • A to T, chromosome 5 at 113,755,446 bp
  • T to G, chromosome 5 at 146,470,356 bp
  • A to G, chromosome 6 at 8,168,102 bp
  • G to A, chromosome 6 at 47,880,168 bp
  • T to A, chromosome 6 at 55,091,292 bp
  • G to A, chromosome 6 at 107,568,527 bp
  • A to T, chromosome 6 at 123,822,917 bp
  • A to T, chromosome 6 at 139,622,139 bp
  • A to G, chromosome 6 at 139,896,792 bp
  • T to C, chromosome 7 at 18,426,380 bp
  • T to C, chromosome 7 at 28,904,619 bp
  • T to A, chromosome 7 at 34,650,122 bp
  • C to T, chromosome 7 at 103,639,679 bp
  • T to A, chromosome 7 at 107,954,794 bp
  • A to G, chromosome 7 at 138,909,304 bp
  • T to A, chromosome 8 at 23,116,098 bp
  • A to T, chromosome 8 at 64,053,891 bp
  • G to A, chromosome 8 at 64,982,883 bp
  • A to T, chromosome 8 at 69,765,723 bp
  • A to T, chromosome 8 at 71,933,413 bp
  • G to T, chromosome 8 at 84,136,942 bp
  • C to T, chromosome 8 at 88,139,954 bp
  • T to A, chromosome 9 at 20,668,528 bp
  • A to G, chromosome 9 at 66,445,549 bp
  • T to C, chromosome 9 at 73,930,884 bp
  • G to A, chromosome 9 at 82,846,563 bp
  • A to T, chromosome 9 at 86,515,343 bp
  • A to G, chromosome 9 at 108,837,151 bp
  • T to C, chromosome 9 at 119,562,374 bp
  • T to C, chromosome 10 at 85,388,083 bp
  • T to A, chromosome 11 at 29,708,023 bp
  • C to T, chromosome 11 at 30,506,354 bp
  • CTGCT to CTGCTTGCT, chromosome 11 at 65,032,084 bp
  • A to G, chromosome 11 at 95,062,715 bp
  • A to G, chromosome 11 at 109,239,560 bp
  • A to T, chromosome 11 at 116,447,006 bp
  • T to C, chromosome 13 at 46,177,443 bp
  • T to C, chromosome 13 at 47,079,253 bp
  • A to C, chromosome 13 at 54,088,942 bp
  • A to T, chromosome 13 at 61,039,141 bp
  • T to A, chromosome 14 at 47,273,210 bp
  • A to G, chromosome 14 at 52,221,071 bp
  • A to T, chromosome 14 at 52,298,786 bp
  • T to C, chromosome 15 at 4,951,281 bp
  • G to T, chromosome 15 at 68,180,777 bp
  • A to T, chromosome 15 at 78,173,781 bp
  • T to G, chromosome 17 at 13,226,134 bp
  • A to G, chromosome 17 at 25,055,589 bp
  • T to A, chromosome 17 at 34,508,397 bp
  • T to A, chromosome 17 at 34,862,102 bp
  • T to C, chromosome 19 at 4,185,031 bp
  • G to A, chromosome 19 at 13,375,334 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6046 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044214-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.