Strain Name:
C57BL/6J-MtgxR6067Btlr
Stock Number:
044231-MU
Citation ID:
RRID:MMRRC_044231-MU
Other Names:
R6067 (G1)
Major Collection:

Gene Information

Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Mgf, Sl, Gb, grizzle-belly, Steel, SCF, Kitlg, blz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Rad23b
Name: RAD23 homolog B, nucleotide excision repair protein
Synonyms: mHR23B, 0610007D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19359
HGNC: HGNC:9813
Homologene: 37704
Zfp1
Name: zinc finger protein 1
Synonyms: mkr-1, Zfp-1, Fnp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22640
Homologene: 32132
Nop14
Name: NOP14 nucleolar protein
Synonyms: 2610033H07Rik, Nol14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 75416
Homologene: 41773
Pgk1
Name: phosphoglycerate kinase 1
Synonyms: Pgk-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18655
HGNC: HGNC:8896
Homologene: 21542
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Adam39
Name: a disintegrin and metallopeptidase domain 39
Synonyms: 1700056P18Rik, testase 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 546055
HGNC: HGNC:199
Homologene: 128364
Theg
Name: testicular haploid expressed gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 21830
Homologene: 7976
Cbfa2t3
Name: CBFA2/RUNX1 translocation partner 3
Synonyms: MTGR2, ETO-2, A630044F12Rik, Eto2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12398
HGNC: HGNC:1537
Homologene: 74543
Tbx5
Name: T-box 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21388
Homologene: 160
Insm2
Name: insulinoma-associated 2
Synonyms: mlt 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56856
VEGA: 12
Homologene: 10651
Satl1
Name: spermidine/spermine N1-acetyl transferase-like 1
Synonyms: 4930404K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 73809
Homologene: 28332
Fam90a1b
Name: family with sequence similarity 90, member A1B
Synonyms: 4932442L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 631145
Homologene: 137326
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 4 at 55,370,400 bp
  • T to C, chromosome 5 at 34,657,951 bp
  • T to C, chromosome 5 at 119,883,146 bp
  • C to T, chromosome 8 at 40,824,593 bp
  • T to C, chromosome 8 at 111,670,343 bp
  • T to C, chromosome 8 at 122,643,497 bp
  • A to G, chromosome 10 at 79,584,755 bp
  • G to A, chromosome 10 at 100,076,906 bp
  • T to C, chromosome 12 at 55,600,014 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • C to A, chromosome 16 at 32,754,529 bp
  • T to A, chromosome 16 at 32,755,247 bp
  • T to C, chromosome X at 94,356,585 bp
  • C to A, chromosome X at 106,194,492 bp
  • T to C, chromosome X at 112,405,916 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6067 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044231-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.