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Strain Name:
C57BL/6J-MtgxR6074Btlr/Mmmh
Stock Number:
044235-MU
Citation ID:
RRID:MMRRC_044235-MU
Other Names:
R6074 (G1)
Major Collection:

Strain Information

Amigo1
Name: adhesion molecule with Ig like domain 1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229715
Homologene: 46421
Kcna6
Name: potassium voltage-gated channel, shaker-related, subfamily, member 6
Synonyms: Kv1.6, MK1.6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16494
HGNC: HGNC:6225
Homologene: 1684
Ip6k1
Name: inositol hexaphosphate kinase 1
Synonyms: InsP6, 1200016D08Rik, Ihpk1, InsP6k1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 27399
Homologene: 56602
Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268
Slc25a12
Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Synonyms: B230107K20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78830
Homologene: 48235
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237898
Homologene: 13066
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,345,379 bp
  • T to A, chromosome 1 at 24,526,483 bp
  • A to G, chromosome 1 at 88,258,664 bp
  • G to A, chromosome 1 at 92,479,775 bp
  • T to A, chromosome 1 at 131,166,342 bp
  • C to T, chromosome 1 at 172,199,185 bp
  • T to A, chromosome 1 at 172,957,378 bp
  • C to T, chromosome 2 at 14,407,479 bp
  • A to G, chromosome 2 at 71,276,454 bp
  • A to C, chromosome 2 at 88,746,222 bp
  • A to G, chromosome 2 at 114,196,389 bp
  • A to G, chromosome 2 at 127,577,873 bp
  • T to A, chromosome 3 at 108,191,693 bp
  • T to A, chromosome 4 at 47,112,405 bp
  • T to C, chromosome 4 at 113,805,200 bp
  • T to C, chromosome 4 at 120,097,694 bp
  • A to G, chromosome 4 at 137,540,735 bp
  • A to T, chromosome 5 at 52,546,642 bp
  • A to G, chromosome 5 at 109,174,301 bp
  • G to T, chromosome 5 at 131,476,989 bp
  • C to A, chromosome 6 at 23,626,671 bp
  • T to A, chromosome 6 at 47,927,052 bp
  • A to G, chromosome 6 at 58,989,738 bp
  • A to G, chromosome 6 at 85,901,187 bp
  • T to A, chromosome 6 at 88,911,937 bp
  • A to G, chromosome 6 at 113,060,010 bp
  • A to G, chromosome 6 at 126,739,279 bp
  • C to T, chromosome 7 at 17,151,559 bp
  • A to G, chromosome 7 at 49,772,574 bp
  • T to A, chromosome 7 at 130,625,435 bp
  • A to G, chromosome 9 at 89,953,915 bp
  • A to G, chromosome 9 at 108,024,109 bp
  • T to A, chromosome 10 at 9,674,590 bp
  • T to A, chromosome 10 at 14,131,741 bp
  • T to C, chromosome 10 at 41,486,065 bp
  • T to A, chromosome 10 at 76,543,859 bp
  • T to A, chromosome 10 at 79,396,543 bp
  • C to T, chromosome 10 at 88,433,078 bp
  • G to T, chromosome 11 at 5,989,635 bp
  • C to T, chromosome 11 at 46,687,172 bp
  • A to G, chromosome 11 at 67,746,814 bp
  • A to G, chromosome 11 at 84,994,573 bp
  • G to A, chromosome 11 at 119,284,186 bp
  • G to A, chromosome 12 at 14,150,511 bp
  • A to G, chromosome 12 at 30,078,187 bp
  • A to G, chromosome 12 at 111,832,402 bp
  • T to C, chromosome 12 at 112,744,155 bp
  • T to C, chromosome 12 at 115,834,387 bp
  • A to T, chromosome 13 at 49,051,399 bp
  • T to C, chromosome 13 at 73,689,763 bp
  • C to A, chromosome 14 at 20,552,099 bp
  • C to T, chromosome 14 at 33,083,639 bp
  • T to C, chromosome 14 at 37,079,643 bp
  • T to G, chromosome 14 at 63,607,070 bp
  • A to G, chromosome 15 at 43,866,738 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • T to A, chromosome 15 at 90,980,892 bp
  • A to C, chromosome 15 at 98,543,324 bp
  • C to A, chromosome 16 at 17,646,727 bp
  • T to C, chromosome 16 at 18,437,547 bp
  • C to T, chromosome 16 at 23,000,596 bp
  • T to A, chromosome 16 at 33,209,965 bp
  • C to T, chromosome 16 at 33,727,203 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6074 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044235-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.