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Strain Name:
C57BL/6J-MtgxR6088Btlr/Mmmh
Stock Number:
044245-MU
Citation ID:
RRID:MMRRC_044245-MU
Other Names:
R6088 (G1)
Major Collection:

Strain Information

Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Elf1
Name: E74 like ETS transcription factor 1
Synonyms: Elf-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13709
VEGA: 14
HGNC: HGNC:3316
Homologene: 7303
Mad1l1
Name: MAD1 mitotic arrest deficient 1-like 1
Synonyms: Mad1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17120
HGNC: HGNC:6762
Homologene: 74500
Pafah2
Name: platelet-activating factor acetylhydrolase 2
Synonyms: 2310074E22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100163
HGNC: HGNC:8579
Homologene: 37309
Rbpj
Name: recombination signal binding protein for immunoglobulin kappa J region
Synonyms: CBF1, Igkrsbp, RBP-J kappa, RBPjk, Igkjrb, Rbpsuh
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19664
HGNC: HGNC:5724
Homologene: 7511
Pibf1
Name: progesterone immunomodulatory binding factor 1
Synonyms: 1700017E21Rik, 4933438D16Rik, 4933439E17Rik, 4930513H15Rik, D14Ertd581e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 52023
VEGA: 14
Homologene: 4628
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: Gtar, 4933425K22Rik, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 88,306,678 bp
  • G to A, chromosome 1 at 180,906,236 bp
  • T to C, chromosome 2 at 32,923,123 bp
  • T to C, chromosome 2 at 38,701,995 bp
  • T to C, chromosome 2 at 52,209,342 bp
  • T to A, chromosome 2 at 85,353,354 bp
  • C to T, chromosome 2 at 120,270,006 bp
  • C to A, chromosome 2 at 121,337,463 bp
  • G to A, chromosome 3 at 30,756,483 bp
  • T to C, chromosome 3 at 82,034,880 bp
  • G to T, chromosome 3 at 86,998,702 bp
  • C to T, chromosome 3 at 153,206,715 bp
  • T to A, chromosome 4 at 3,595,383 bp
  • G to A, chromosome 4 at 108,012,444 bp
  • G to A, chromosome 4 at 118,210,755 bp
  • G to T, chromosome 4 at 132,332,842 bp
  • A to T, chromosome 4 at 134,075,790 bp
  • A to G, chromosome 4 at 134,413,381 bp
  • C to A, chromosome 5 at 45,584,012 bp
  • C to T, chromosome 5 at 53,651,368 bp
  • C to T, chromosome 5 at 65,349,013 bp
  • T to A, chromosome 5 at 89,197,704 bp
  • C to T, chromosome 5 at 90,253,688 bp
  • C to T, chromosome 5 at 108,674,006 bp
  • T to C, chromosome 5 at 135,134,030 bp
  • T to A, chromosome 5 at 140,193,963 bp
  • A to T, chromosome 6 at 29,502,632 bp
  • T to C, chromosome 6 at 90,203,100 bp
  • G to A, chromosome 7 at 3,611,312 bp
  • A to T, chromosome 7 at 12,775,198 bp
  • T to A, chromosome 7 at 25,080,339 bp
  • C to T, chromosome 7 at 28,361,013 bp
  • T to A, chromosome 7 at 56,985,078 bp
  • A to T, chromosome 7 at 64,267,976 bp
  • G to T, chromosome 8 at 123,460,048 bp
  • TCTCCTC to TCTC, chromosome 10 at 128,114,642 bp
  • A to G, chromosome 11 at 32,212,007 bp
  • A to T, chromosome 11 at 73,928,823 bp
  • A to G, chromosome 11 at 99,073,720 bp
  • A to G, chromosome 12 at 31,340,989 bp
  • A to G, chromosome 12 at 71,022,236 bp
  • T to C, chromosome 13 at 61,253,966 bp
  • T to A, chromosome 14 at 54,367,694 bp
  • A to T, chromosome 14 at 57,816,694 bp
  • C to T, chromosome 14 at 79,567,261 bp
  • T to A, chromosome 14 at 99,179,358 bp
  • A to T, chromosome 15 at 5,105,019 bp
  • T to A, chromosome 15 at 102,208,808 bp
  • A to T, chromosome 16 at 5,104,988 bp
  • G to A, chromosome 16 at 10,511,931 bp
  • A to T, chromosome 16 at 19,673,398 bp
  • G to A, chromosome 17 at 27,884,605 bp
  • A to G, chromosome 17 at 39,040,010 bp
  • T to C, chromosome 17 at 71,255,124 bp
  • G to T, chromosome 17 at 83,503,972 bp
  • A to C, chromosome 18 at 13,846,109 bp
  • G to T, chromosome 18 at 58,902,102 bp
  • T to A, chromosome 18 at 74,720,898 bp
  • A to G, chromosome 19 at 44,555,025 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6088 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044245-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.