Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6127Btlr/Mmmh
Stock Number:
044274-MU
Citation ID:
RRID:MMRRC_044274-MU
Other Names:
R6127 (G1)
Major Collection:

Strain Information

Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Ddx50
Name: DExD box helicase 50
Synonyms: GU2, RH-II/Gubeta, 4933429B04Rik, 8430408E17Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 94213
VEGA: 10
Homologene: 56986
Ireb2
Name: iron responsive element binding protein 2
Synonyms: Irp2, D9Ertd85e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 64602
VEGA: 9
HGNC: HGNC:6115
Homologene: 11280
Carmil1
Name: capping protein regulator and myosin 1 linker 1
Synonyms: 1110037D04Rik, Lrrc16, Carmil, Lrrc16a
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68732
Homologene: 9757
Fblim1
Name: filamin binding LIM protein 1
Synonyms: 2410043F08Rik, migfilin(s), migfilin, Fblp1, Cal
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74202
Homologene: 56774
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57752
Homologene: 5087
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 4,349,311 bp
  • T to C, chromosome 1 at 88,234,612 bp
  • A to G, chromosome 2 at 4,763,277 bp
  • T to A, chromosome 2 at 87,949,361 bp
  • G to T, chromosome 2 at 128,738,291 bp
  • G to A, chromosome 2 at 136,087,406 bp
  • A to G, chromosome 2 at 154,549,589 bp
  • G to A, chromosome 3 at 51,530,081 bp
  • C to T, chromosome 3 at 66,994,649 bp
  • T to A, chromosome 3 at 116,758,329 bp
  • G to T, chromosome 4 at 63,368,119 bp
  • T to A, chromosome 4 at 135,934,811 bp
  • G to A, chromosome 4 at 141,584,722 bp
  • A to G, chromosome 5 at 24,325,003 bp
  • C to T, chromosome 5 at 33,335,518 bp
  • T to G, chromosome 5 at 84,071,094 bp
  • T to C, chromosome 6 at 40,449,767 bp
  • C to T, chromosome 6 at 113,813,877 bp
  • G to A, chromosome 7 at 3,662,867 bp
  • A to T, chromosome 7 at 10,105,631 bp
  • T to A, chromosome 7 at 17,211,746 bp
  • T to C, chromosome 7 at 44,542,121 bp
  • T to C, chromosome 7 at 52,003,453 bp
  • T to A, chromosome 7 at 84,857,202 bp
  • A to T, chromosome 7 at 130,626,115 bp
  • T to C, chromosome 8 at 36,147,401 bp
  • T to A, chromosome 9 at 18,657,878 bp
  • T to A, chromosome 9 at 46,308,922 bp
  • A to C, chromosome 9 at 53,524,509 bp
  • A to G, chromosome 9 at 54,882,368 bp
  • A to T, chromosome 9 at 65,866,470 bp
  • T to C, chromosome 9 at 95,969,489 bp
  • T to C, chromosome 9 at 100,951,697 bp
  • A to T, chromosome 10 at 62,621,563 bp
  • G to A, chromosome 10 at 81,324,309 bp
  • A to G, chromosome 10 at 129,911,415 bp
  • C to A, chromosome 11 at 49,430,806 bp
  • T to C, chromosome 11 at 121,216,999 bp
  • A to T, chromosome 13 at 22,395,203 bp
  • T to C, chromosome 13 at 24,036,352 bp
  • A to G, chromosome 14 at 53,845,542 bp
  • A to G, chromosome 14 at 84,533,060 bp
  • T to A, chromosome 15 at 47,650,228 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • G to A, chromosome 16 at 33,798,947 bp
  • A to T, chromosome 16 at 73,013,068 bp
  • G to T, chromosome 17 at 24,861,004 bp
  • T to A, chromosome 17 at 28,356,150 bp
  • T to C, chromosome 17 at 33,433,380 bp
  • T to A, chromosome 17 at 57,034,914 bp
  • A to T, chromosome 18 at 37,674,704 bp
  • C to A, chromosome 18 at 37,732,879 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6127 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044274-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.