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Strain Name:
C57BL/6J-MtgxR6147Btlr/Mmmh
Stock Number:
044294-MU
Citation ID:
RRID:MMRRC_044294-MU
Other Names:
R6147 (G1)
Major Collection:

Strain Information

Arfgef2
Name: ARF guanine nucleotide exchange factor 2
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99371
Homologene: 111880
Bclaf1
Name: BCL2-associated transcription factor 1
Synonyms: 2700025J07Rik, 2810454G14Rik, 2610102K23Rik, 5730534O06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72567
Homologene: 8832
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Luzp1
Name: leucine zipper protein 1
Synonyms: Luzp, 2700072H04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269593
Homologene: 11545
Stx16
Name: syntaxin 16
Synonyms: 5430410K23Rik, SYN16, 6330500A18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228960
Homologene: 2791
Set
Name: SET nuclear oncogene
Synonyms: StF-IT-1, 5730420M11Rik, 2610030F17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56086
Homologene: 55707
Ranbp2
Name: RAN binding protein 2
Synonyms: A430087B05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19386
VEGA: 10
HGNC: HGNC:9848
Homologene: 87808
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 45,819,538 bp
  • G to A, chromosome 1 at 100,050,781 bp
  • C to T, chromosome 1 at 127,729,420 bp
  • G to A, chromosome 1 at 136,345,400 bp
  • A to T, chromosome 1 at 156,318,726 bp
  • T to A, chromosome 2 at 30,066,824 bp
  • A to C, chromosome 2 at 35,165,733 bp
  • A to T, chromosome 2 at 86,332,500 bp
  • C to T, chromosome 2 at 87,868,717 bp
  • A to G, chromosome 2 at 166,871,495 bp
  • A to G, chromosome 2 at 174,090,687 bp
  • A to T, chromosome 2 at 177,832,807 bp
  • A to T, chromosome 3 at 30,659,079 bp
  • A to G, chromosome 3 at 63,722,881 bp
  • A to G, chromosome 3 at 84,529,178 bp
  • A to G, chromosome 3 at 132,694,446 bp
  • A to G, chromosome 4 at 136,541,063 bp
  • T to C, chromosome 4 at 152,315,655 bp
  • C to T, chromosome 5 at 109,054,834 bp
  • G to A, chromosome 5 at 142,497,940 bp
  • T to C, chromosome 5 at 147,548,283 bp
  • C to T, chromosome 6 at 4,754,499 bp
  • A to G, chromosome 6 at 41,059,703 bp
  • A to T, chromosome 6 at 41,616,781 bp
  • G to T, chromosome 6 at 55,336,610 bp
  • T to C, chromosome 7 at 14,465,163 bp
  • A to G, chromosome 7 at 15,626,001 bp
  • A to T, chromosome 7 at 29,085,914 bp
  • A to G, chromosome 7 at 48,552,365 bp
  • A to T, chromosome 7 at 79,758,497 bp
  • A to T, chromosome 7 at 104,261,191 bp
  • T to A, chromosome 8 at 14,727,294 bp
  • T to A, chromosome 8 at 69,102,482 bp
  • A to T, chromosome 8 at 75,054,692 bp
  • C to T, chromosome 8 at 77,667,322 bp
  • T to A, chromosome 8 at 122,781,474 bp
  • T to A, chromosome 9 at 10,012,889 bp
  • A to T, chromosome 9 at 35,742,110 bp
  • A to G, chromosome 9 at 35,742,232 bp
  • A to T, chromosome 9 at 56,888,772 bp
  • G to T, chromosome 9 at 97,363,329 bp
  • A to T, chromosome 10 at 9,808,472 bp
  • T to A, chromosome 10 at 20,323,425 bp
  • A to T, chromosome 10 at 39,921,577 bp
  • C to A, chromosome 10 at 58,479,428 bp
  • G to A, chromosome 10 at 107,777,117 bp
  • A to G, chromosome 11 at 48,915,890 bp
  • T to A, chromosome 11 at 60,949,950 bp
  • T to C, chromosome 11 at 100,188,839 bp
  • T to C, chromosome 11 at 104,967,740 bp
  • A to G, chromosome 12 at 55,379,308 bp
  • T to C, chromosome 12 at 76,565,211 bp
  • T to A, chromosome 12 at 91,538,235 bp
  • T to A, chromosome 12 at 114,094,220 bp
  • C to A, chromosome 14 at 21,848,815 bp
  • G to C, chromosome 14 at 55,887,603 bp
  • A to T, chromosome 14 at 103,155,509 bp
  • T to A, chromosome 15 at 35,930,031 bp
  • T to A, chromosome 15 at 55,648,010 bp
  • A to T, chromosome 17 at 7,753,762 bp
  • T to C, chromosome 17 at 34,418,923 bp
  • T to C, chromosome 17 at 37,466,539 bp
  • T to A, chromosome 18 at 37,320,726 bp
  • G to T, chromosome 18 at 37,402,441 bp
  • T to A, chromosome 18 at 61,201,685 bp
  • T to A, chromosome 19 at 6,337,242 bp
  • G to A, chromosome 19 at 11,747,572 bp
  • T to G, chromosome 19 at 12,462,894 bp
  • A to G, chromosome 19 at 38,702,037 bp
  • C to G, chromosome X at 9,450,750 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6147 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044294-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.