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Strain Name:
C57BL/6J-MtgxR6148Btlr/Mmmh
Stock Number:
044295-MU
Citation ID:
RRID:MMRRC_044295-MU
Other Names:
R6148 (G1)
Major Collection:

Strain Information

Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Cyp19a1
Name: cytochrome P450, family 19, subfamily a, polypeptide 1
Synonyms: Int-5, Int5, aromatase, Ar, Cyp19, ArKO, p450arom
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13075
VEGA: 9
HGNC: HGNC:2594
Homologene: 30955
Galnt10
Name: polypeptide N-acetylgalactosaminyltransferase 10
Synonyms: GalNAc-T10, Galnt9, C330012K04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 171212
Homologene: 14924
Dclre1c
Name: DNA cross-link repair 1C
Synonyms: 9930121L06Rik, Artemis, Art
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227525
Homologene: 32547
Hap1
Name: huntingtin-associated protein 1
Synonyms: HAP-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15114
HGNC: HGNC:4812
Homologene: 2935
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Ese1, EHSH1, Sh3p17, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 73,953,453 bp
  • T to C, chromosome 1 at 125,872,586 bp
  • T to G, chromosome 1 at 162,851,519 bp
  • G to A, chromosome 1 at 172,134,039 bp
  • T to C, chromosome 1 at 174,666,663 bp
  • T to C, chromosome 1 at 194,948,008 bp
  • A to G, chromosome 2 at 3,437,705 bp
  • G to A, chromosome 2 at 28,693,781 bp
  • ATCTCTCTCTCTCTCTCT to ATCTCTCTCTCTCTCT, chromosome 2 at 77,929,459 bp
  • C to T, chromosome 2 at 88,930,253 bp
  • T to G, chromosome 2 at 104,992,137 bp
  • G to A, chromosome 2 at 181,187,848 bp
  • C to A, chromosome 3 at 36,613,266 bp
  • T to C, chromosome 4 at 129,321,958 bp
  • G to A, chromosome 4 at 129,649,306 bp
  • A to G, chromosome 4 at 134,217,929 bp
  • A to G, chromosome 4 at 143,132,907 bp
  • T to C, chromosome 4 at 144,721,317 bp
  • A to G, chromosome 4 at 155,854,952 bp
  • T to C, chromosome 5 at 5,663,996 bp
  • A to G, chromosome 5 at 21,226,325 bp
  • A to T, chromosome 5 at 21,270,577 bp
  • A to T, chromosome 5 at 70,774,461 bp
  • A to T, chromosome 5 at 87,579,911 bp
  • A to T, chromosome 5 at 90,759,706 bp
  • A to T, chromosome 5 at 136,982,633 bp
  • T to C, chromosome 6 at 29,535,959 bp
  • T to A, chromosome 6 at 34,857,753 bp
  • A to G, chromosome 6 at 148,799,780 bp
  • T to C, chromosome 7 at 10,171,163 bp
  • C to A, chromosome 7 at 12,668,142 bp
  • A to T, chromosome 7 at 19,429,516 bp
  • A to T, chromosome 7 at 24,744,273 bp
  • A to G, chromosome 7 at 80,380,296 bp
  • A to T, chromosome 7 at 98,443,823 bp
  • A to G, chromosome 7 at 104,881,082 bp
  • A to T, chromosome 7 at 108,844,971 bp
  • A to G, chromosome 7 at 141,106,785 bp
  • A to G, chromosome 8 at 21,202,412 bp
  • A to G, chromosome 8 at 123,127,432 bp
  • T to A, chromosome 9 at 7,849,683 bp
  • G to T, chromosome 9 at 39,647,259 bp
  • C to T, chromosome 9 at 54,180,256 bp
  • G to C, chromosome 9 at 73,693,366 bp
  • T to C, chromosome 10 at 127,099,943 bp
  • T to C, chromosome 11 at 57,784,648 bp
  • A to T, chromosome 11 at 100,349,392 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • T to C, chromosome 12 at 4,850,621 bp
  • T to C, chromosome 12 at 24,651,568 bp
  • T to A, chromosome 12 at 71,187,426 bp
  • A to G, chromosome 14 at 50,534,321 bp
  • T to C, chromosome 16 at 32,753,802 bp
  • C to T, chromosome 16 at 91,816,852 bp
  • G to A, chromosome 17 at 34,645,329 bp
  • A to G, chromosome 18 at 37,449,230 bp
  • A to T, chromosome 19 at 3,283,084 bp
  • C to T, chromosome 19 at 8,804,196 bp
  • G to A, chromosome 19 at 10,212,475 bp
  • G to A, chromosome 19 at 15,981,786 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6148 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044295-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.