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Strain Name:
C57BL/6J-MtgxR6151Btlr/Mmmh
Stock Number:
044298-MU
Citation ID:
RRID:MMRRC_044298-MU
Other Names:
R6151 (G1)
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Epha2
Name: Eph receptor A2
Synonyms: Sek-2, Eck, Sek2, Myk2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13836
HGNC: HGNC:3386
Homologene: 20929
Cd36
Name: CD36 molecule
Synonyms: FAT, fatty acid translocase, Scarb3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12491
HGNC: HGNC:1663
Homologene: 73871
Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Lefty1
Name: left right determination factor 1
Synonyms: lefty-1, Stra3, Lefty, Ebaf
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13590
HGNC: HGNC:6552
Homologene: 49231
Trmo
Name: tRNA methyltransferase O
Synonyms: 5830415F09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74753
Homologene: 32316
Traip
Name: TRAF-interacting protein
Synonyms: Trip
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22036
Homologene: 31343
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 90,813,753 bp
  • A to G, chromosome 1 at 120,171,486 bp
  • T to A, chromosome 1 at 134,967,960 bp
  • A to T, chromosome 1 at 164,181,635 bp
  • G to A, chromosome 1 at 164,190,187 bp
  • T to C, chromosome 1 at 180,935,116 bp
  • G to T, chromosome 1 at 185,407,754 bp
  • A to T, chromosome 1 at 192,759,757 bp
  • T to A, chromosome 2 at 20,713,360 bp
  • T to C, chromosome 2 at 53,157,915 bp
  • T to G, chromosome 2 at 69,824,854 bp
  • A to T, chromosome 2 at 76,944,160 bp
  • T to A, chromosome 2 at 90,690,105 bp
  • A to C, chromosome 2 at 91,165,457 bp
  • T to A, chromosome 2 at 121,289,823 bp
  • T to A, chromosome 3 at 116,507,827 bp
  • T to C, chromosome 3 at 122,570,930 bp
  • T to C, chromosome 3 at 129,332,418 bp
  • C to T, chromosome 3 at 145,314,054 bp
  • A to C, chromosome 4 at 21,810,797 bp
  • T to A, chromosome 4 at 32,684,735 bp
  • G to A, chromosome 4 at 46,389,390 bp
  • A to G, chromosome 4 at 53,085,261 bp
  • T to A, chromosome 4 at 102,601,551 bp
  • C to A, chromosome 4 at 119,623,271 bp
  • G to T, chromosome 4 at 133,684,976 bp
  • A to T, chromosome 4 at 140,796,394 bp
  • A to G, chromosome 4 at 141,318,480 bp
  • A to T, chromosome 5 at 3,559,133 bp
  • A to G, chromosome 5 at 6,769,910 bp
  • A to G, chromosome 5 at 9,511,556 bp
  • T to C, chromosome 5 at 17,795,595 bp
  • G to T, chromosome 5 at 27,761,618 bp
  • A to T, chromosome 5 at 65,417,581 bp
  • T to A, chromosome 5 at 140,705,961 bp
  • T to A, chromosome 5 at 144,737,851 bp
  • T to A, chromosome 5 at 150,988,853 bp
  • A to G, chromosome 6 at 125,657,065 bp
  • G to T, chromosome 7 at 12,361,914 bp
  • A to T, chromosome 7 at 18,069,105 bp
  • C to A, chromosome 7 at 19,491,745 bp
  • T to A, chromosome 7 at 20,101,435 bp
  • A to G, chromosome 7 at 24,917,942 bp
  • T to A, chromosome 7 at 86,801,670 bp
  • C to T, chromosome 7 at 99,690,563 bp
  • G to A, chromosome 7 at 105,574,252 bp
  • A to G, chromosome 7 at 108,055,899 bp
  • G to A, chromosome 7 at 113,561,396 bp
  • A to G, chromosome 7 at 126,775,502 bp
  • G to A, chromosome 7 at 127,207,996 bp
  • A to T, chromosome 7 at 139,921,213 bp
  • C to T, chromosome 8 at 48,395,573 bp
  • T to A, chromosome 8 at 69,817,085 bp
  • A to C, chromosome 9 at 48,326,191 bp
  • G to T, chromosome 9 at 53,414,383 bp
  • G to T, chromosome 9 at 107,970,619 bp
  • T to C, chromosome 10 at 52,304,660 bp
  • T to C, chromosome 10 at 80,669,025 bp
  • A to C, chromosome 11 at 36,008,783 bp
  • T to A, chromosome 11 at 73,787,695 bp
  • T to G, chromosome 11 at 75,156,207 bp
  • G to A, chromosome 11 at 78,036,982 bp
  • T to A, chromosome 11 at 83,017,321 bp
  • A to G, chromosome 11 at 97,500,412 bp
  • C to T, chromosome 11 at 99,075,550 bp
  • T to C, chromosome 11 at 99,337,489 bp
  • C to A, chromosome 11 at 102,008,566 bp
  • T to A, chromosome 11 at 114,864,025 bp
  • T to C, chromosome 12 at 25,056,909 bp
  • A to C, chromosome 12 at 53,025,792 bp
  • T to A, chromosome 12 at 104,152,068 bp
  • C to A, chromosome 12 at 104,317,390 bp
  • C to A, chromosome 12 at 115,461,289 bp
  • T to G, chromosome 12 at 119,261,106 bp
  • A to T, chromosome 13 at 66,431,681 bp
  • T to A, chromosome 13 at 67,257,598 bp
  • T to G, chromosome 13 at 97,093,630 bp
  • A to G, chromosome 13 at 112,689,376 bp
  • A to G, chromosome 14 at 52,295,020 bp
  • A to T, chromosome 14 at 60,244,616 bp
  • A to T, chromosome 15 at 76,594,687 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • G to C, chromosome 15 at 81,778,710 bp
  • T to C, chromosome 15 at 98,878,279 bp
  • A to C, chromosome 16 at 10,292,281 bp
  • A to T, chromosome 16 at 19,515,531 bp
  • T to C, chromosome 16 at 35,817,222 bp
  • A to T, chromosome 16 at 95,295,668 bp
  • A to T, chromosome 17 at 18,583,959 bp
  • A to G, chromosome 17 at 24,575,606 bp
  • G to T, chromosome 17 at 34,212,047 bp
  • A to T, chromosome 17 at 47,689,088 bp
  • A to T, chromosome 18 at 10,106,426 bp
  • A to T, chromosome 18 at 34,494,277 bp
  • T to G, chromosome 18 at 37,853,353 bp
  • C to T, chromosome 19 at 7,619,291 bp
  • T to C, chromosome 19 at 12,036,736 bp
  • A to G, chromosome 19 at 56,903,465 bp
  • C to T, chromosome X at 170,676,467 bp
  • A to G, chromosome Y at 825,984 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6151 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044298-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.