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Strain Name:
C57BL/6J-MtgxR6154Btlr/Mmmh
Stock Number:
044301-MU
Citation ID:
RRID:MMRRC_044301-MU
Other Names:
R6154 (G1)
Major Collection:

Strain Information

Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Igf2bp2
Name: insulin-like growth factor 2 mRNA binding protein 2
Synonyms: IMP-2, C330012H03Rik, IMP2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 319765
Homologene: 4774
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 328329
Homologene: 42094
Asxl2
Name: ASXL transcriptional regulator 2
Synonyms: 4930556B16Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 75302
Homologene: 10102
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 18,122,788 bp
  • A to T, chromosome 1 at 33,803,546 bp
  • T to C, chromosome 1 at 75,500,144 bp
  • T to A, chromosome 1 at 90,773,665 bp
  • C to T, chromosome 1 at 130,598,905 bp
  • T to A, chromosome 1 at 150,423,816 bp
  • T to G, chromosome 2 at 87,309,558 bp
  • A to G, chromosome 2 at 87,782,756 bp
  • A to G, chromosome 2 at 88,910,390 bp
  • A to G, chromosome 3 at 14,481,034 bp
  • T to A, chromosome 3 at 63,300,253 bp
  • C to A, chromosome 3 at 141,678,153 bp
  • T to C, chromosome 3 at 142,277,913 bp
  • C to A, chromosome 4 at 43,482,981 bp
  • A to G, chromosome 4 at 86,583,756 bp
  • A to T, chromosome 4 at 88,603,294 bp
  • C to A, chromosome 4 at 147,674,688 bp
  • A to G, chromosome 5 at 36,476,664 bp
  • C to T, chromosome 5 at 87,979,754 bp
  • T to C, chromosome 5 at 110,755,933 bp
  • C to T, chromosome 5 at 111,338,397 bp
  • T to C, chromosome 5 at 124,378,479 bp
  • T to A, chromosome 5 at 138,064,485 bp
  • A to G, chromosome 5 at 139,307,776 bp
  • A to G, chromosome 6 at 14,754,604 bp
  • A to G, chromosome 6 at 113,495,028 bp
  • T to C, chromosome 7 at 7,291,482 bp
  • A to T, chromosome 7 at 10,087,994 bp
  • T to C, chromosome 7 at 23,660,158 bp
  • C to A, chromosome 7 at 43,412,017 bp
  • A to AAGGCGACGG, chromosome 7 at 97,579,904 bp
  • GCG to GCGACGGCGACG, chromosome 7 at 97,579,907 bp
  • T to A, chromosome 7 at 131,109,641 bp
  • A to G, chromosome 8 at 13,768,609 bp
  • A to G, chromosome 8 at 116,515,689 bp
  • G to T, chromosome 8 at 125,762,813 bp
  • A to T, chromosome 9 at 105,323,169 bp
  • T to G, chromosome 10 at 62,468,340 bp
  • C to T, chromosome 10 at 81,626,505 bp
  • T to A, chromosome 10 at 92,698,242 bp
  • T to G, chromosome 10 at 107,123,349 bp
  • C to T, chromosome 10 at 129,487,676 bp
  • T to A, chromosome 11 at 32,603,654 bp
  • T to G, chromosome 11 at 65,855,338 bp
  • A to G, chromosome 11 at 67,186,612 bp
  • C to T, chromosome 11 at 69,856,954 bp
  • T to C, chromosome 11 at 70,610,101 bp
  • G to A, chromosome 11 at 70,678,385 bp
  • T to A, chromosome 11 at 73,049,874 bp
  • A to G, chromosome 11 at 103,614,143 bp
  • A to G, chromosome 11 at 119,278,352 bp
  • A to T, chromosome 11 at 121,221,271 bp
  • T to C, chromosome 12 at 3,496,593 bp
  • T to C, chromosome 12 at 110,617,993 bp
  • C to T, chromosome 12 at 113,545,672 bp
  • T to C, chromosome 12 at 114,416,742 bp
  • T to A, chromosome 13 at 9,878,440 bp
  • A to G, chromosome 13 at 102,787,421 bp
  • T to A, chromosome 14 at 43,076,563 bp
  • C to T, chromosome 14 at 48,250,594 bp
  • C to A, chromosome 14 at 67,859,912 bp
  • T to G, chromosome 15 at 28,204,031 bp
  • T to C, chromosome 15 at 42,523,655 bp
  • T to C, chromosome 15 at 73,058,081 bp
  • T to C, chromosome 15 at 76,248,887 bp
  • A to T, chromosome 16 at 19,286,431 bp
  • G to T, chromosome 16 at 22,076,093 bp
  • A to G, chromosome 16 at 76,293,830 bp
  • T to C, chromosome 17 at 25,157,954 bp
  • A to G, chromosome 18 at 37,334,913 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • T to C, chromosome 19 at 8,851,888 bp
  • T to C, chromosome 19 at 22,987,814 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6154 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044301-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.