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Strain Name:
C57BL/6J-MtgxR6194Btlr/Mmmh
Stock Number:
044334-MU
Citation ID:
RRID:MMRRC_044334-MU
Other Names:
R6194 (G1)
Major Collection:

Strain Information

Baz1b
Name: bromodomain adjacent to zinc finger domain, 1B
Synonyms: Wbscr9, WSTF
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22385
HGNC: HGNC:961
Homologene: 22651
Naa15
Name: N(alpha)-acetyltransferase 15, NatA auxiliary subunit
Synonyms: tubedown, Tbdn-1, mNAT1, 5730450D16Rik, Narg1, ASTBDN
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74838
Homologene: 14211
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Rbfox2
Name: RNA binding protein, fox-1 homolog (C. elegans) 2
Synonyms: Fxh, 2810460A15Rik, Fbm2, Rbm9
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 93686
VEGA: 15
HGNC: HGNC:9906
Homologene: 49375
Asap1
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Synonyms: Ddef1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13196
HGNC: HGNC:2720
Homologene: 7684
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Hunk
Name: hormonally upregulated Neu-associated kinase
Synonyms: Mak-v, Bstk1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 26559
VEGA: 16
Homologene: 8742
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 60,257,484 bp
  • T to A, chromosome 1 at 105,991,117 bp
  • A to G, chromosome 1 at 130,697,887 bp
  • G to A, chromosome 2 at 34,807,404 bp
  • A to G, chromosome 2 at 73,570,472 bp
  • C to A, chromosome 2 at 92,389,244 bp
  • A to T, chromosome 2 at 150,472,631 bp
  • A to G, chromosome 3 at 51,463,300 bp
  • C to A, chromosome 3 at 103,058,953 bp
  • A to G, chromosome 3 at 106,404,876 bp
  • T to C, chromosome 3 at 108,465,779 bp
  • C to T, chromosome 4 at 46,629,912 bp
  • T to A, chromosome 4 at 52,825,779 bp
  • T to A, chromosome 4 at 88,691,125 bp
  • G to T, chromosome 4 at 88,691,126 bp
  • T to A, chromosome 4 at 154,883,216 bp
  • A to G, chromosome 4 at 155,866,641 bp
  • T to C, chromosome 5 at 26,104,035 bp
  • T to C, chromosome 5 at 31,666,890 bp
  • C to A, chromosome 5 at 39,614,405 bp
  • A to T, chromosome 5 at 89,441,579 bp
  • C to T, chromosome 5 at 129,990,065 bp
  • T to C, chromosome 5 at 135,243,890 bp
  • C to CCCATCAGGA, chromosome 6 at 4,756,351 bp
  • C to T, chromosome 6 at 68,939,932 bp
  • A to T, chromosome 7 at 3,629,027 bp
  • T to A, chromosome 7 at 43,435,662 bp
  • G to T, chromosome 7 at 105,122,170 bp
  • T to C, chromosome 7 at 110,899,694 bp
  • C to T, chromosome 7 at 126,692,331 bp
  • T to C, chromosome 7 at 141,205,651 bp
  • A to G, chromosome 8 at 112,875,429 bp
  • T to A, chromosome 9 at 15,726,876 bp
  • C to T, chromosome 9 at 18,351,147 bp
  • A to G, chromosome 9 at 59,869,750 bp
  • A to G, chromosome 10 at 9,817,339 bp
  • T to C, chromosome 10 at 44,294,616 bp
  • T to A, chromosome 11 at 50,383,277 bp
  • T to A, chromosome 12 at 24,946,638 bp
  • A to T, chromosome 12 at 51,748,445 bp
  • T to A, chromosome 12 at 104,266,503 bp
  • T to A, chromosome 13 at 14,637,120 bp
  • A to T, chromosome 15 at 5,098,444 bp
  • A to G, chromosome 15 at 64,129,209 bp
  • T to C, chromosome 15 at 77,084,157 bp
  • A to G, chromosome 16 at 3,929,906 bp
  • G to A, chromosome 16 at 4,054,800 bp
  • A to T, chromosome 16 at 18,072,517 bp
  • G to A, chromosome 16 at 29,308,239 bp
  • A to G, chromosome 16 at 52,268,398 bp
  • A to G, chromosome 16 at 87,415,810 bp
  • A to T, chromosome 16 at 90,496,395 bp
  • A to G, chromosome 18 at 37,342,146 bp
  • C to A, chromosome 19 at 3,262,003 bp
  • G to T, chromosome 19 at 5,770,306 bp
  • T to C, chromosome 19 at 34,587,627 bp
  • T to A, chromosome 19 at 56,922,951 bp
  • G to T, chromosome X at 20,860,100 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6194 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044334-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.