Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR6195Btlr
Stock Number:
044335-MU
Citation ID:
RRID:MMRRC_044335-MU
Other Names:
R6195 (G1)
Major Collection:
Beutler Mutagenetix

Gene Information

Gene Details

Gene Symbol: Arhgef7 [MGI:1860493] (Mus musculus (mouse))
Name: Rho guanine nucleotide exchange factor (GEF7)
Synonyms: cool-1, betaPix-b, Pak interacting exchange factor, betaPix, Cool, betaPix-c, PIX, p85SPR
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Serpina9 [MGI:1919157] (Mus musculus (mouse))
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 9
Synonyms: Centerin, 2310014L03Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Nf1 [MGI:97306] (Mus musculus (mouse))
Name: neurofibromin 1
Synonyms: Nf-1, neurofibromin, Dsk9, Mhdadsk9
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Uaca [MGI:1919815] (Mus musculus (mouse))
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: nucling, 2700059D02Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Fxr2 [MGI:1346074] (Mus musculus (mouse))
Name: fragile X mental retardation, autosomal homolog 2
Synonyms: Fxr2h
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Mical3 [MGI:2442733] (Mus musculus (mouse))
Name: microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms: MICAL-3, C130040D16Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Atp5c1 [MGI:1261437] (Mus musculus (mouse))
Name: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
Synonyms: 1700094F02Rik, F1 gamma
Chromosome: 2
Alteration at locus: Chemically Induced
See All Alterations
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,375,286 bp
  • G to A, chromosome 1 at 46,204,269 bp
  • A to G, chromosome 1 at 193,174,834 bp
  • T to C, chromosome 2 at 10,064,115 bp
  • C to A, chromosome 2 at 15,695,326 bp
  • T to C, chromosome 2 at 31,384,115 bp
  • T to A, chromosome 2 at 59,907,511 bp
  • T to A, chromosome 2 at 66,277,618 bp
  • T to A, chromosome 2 at 76,737,653 bp
  • T to C, chromosome 2 at 86,413,207 bp
  • T to C, chromosome 2 at 87,868,560 bp
  • T to A, chromosome 2 at 90,813,313 bp
  • A to T, chromosome 2 at 120,392,175 bp
  • G to A, chromosome 3 at 59,752,202 bp
  • G to T, chromosome 3 at 63,740,789 bp
  • G to A, chromosome 3 at 103,337,532 bp
  • A to G, chromosome 4 at 43,566,346 bp
  • C to T, chromosome 4 at 46,629,912 bp
  • A to G, chromosome 4 at 122,999,920 bp
  • T to A, chromosome 4 at 134,926,802 bp
  • T to C, chromosome 5 at 43,979,362 bp
  • T to C, chromosome 5 at 114,767,114 bp
  • T to A, chromosome 5 at 138,342,275 bp
  • T to A, chromosome 5 at 142,765,173 bp
  • T to A, chromosome 5 at 145,243,762 bp
  • C to A, chromosome 6 at 48,908,677 bp
  • G to A, chromosome 6 at 52,245,701 bp
  • G to T, chromosome 6 at 66,537,628 bp
  • A to T, chromosome 6 at 67,896,491 bp
  • T to C, chromosome 6 at 80,021,956 bp
  • T to C, chromosome 6 at 88,840,736 bp
  • T to A, chromosome 6 at 121,016,835 bp
  • A to T, chromosome 7 at 64,354,371 bp
  • A to T, chromosome 7 at 104,179,754 bp
  • A to G, chromosome 7 at 127,028,959 bp
  • T to C, chromosome 7 at 134,918,648 bp
  • C to T, chromosome 8 at 11,822,017 bp
  • A to G, chromosome 8 at 72,762,885 bp
  • A to T, chromosome 8 at 80,879,532 bp
  • A to G, chromosome 8 at 84,588,753 bp
  • T to A, chromosome 9 at 21,731,781 bp
  • G to A, chromosome 9 at 60,870,044 bp
  • A to G, chromosome 9 at 119,137,253 bp
  • T to A, chromosome 10 at 45,670,443 bp
  • T to C, chromosome 10 at 58,270,984 bp
  • A to G, chromosome 11 at 31,666,740 bp
  • T to A, chromosome 11 at 58,997,207 bp
  • C to T, chromosome 11 at 69,652,273 bp
  • A to G, chromosome 11 at 79,565,975 bp
  • T to A, chromosome 11 at 119,210,565 bp
  • A to G, chromosome 12 at 72,556,874 bp
  • T to A, chromosome 12 at 104,001,407 bp
  • A to G, chromosome 13 at 54,784,205 bp
  • T to C, chromosome 13 at 55,523,576 bp
  • G to T, chromosome 13 at 91,208,436 bp
  • T to A, chromosome 13 at 112,964,537 bp
  • T to G, chromosome 14 at 43,544,631 bp
  • T to A, chromosome 14 at 60,872,105 bp
  • T to C, chromosome 14 at 88,468,052 bp
  • T to C, chromosome 15 at 58,637,957 bp
  • A to T, chromosome 15 at 89,122,791 bp
  • C to T, chromosome 16 at 5,203,749 bp
  • C to T, chromosome 16 at 22,932,331 bp
  • G to A, chromosome 16 at 32,084,586 bp
  • T to C, chromosome 16 at 57,231,350 bp
  • A to G, chromosome 16 at 59,329,422 bp
  • T to C, chromosome 17 at 27,086,960 bp
  • T to C, chromosome 17 at 33,919,982 bp
  • A to G, chromosome 17 at 43,629,752 bp
  • A to G, chromosome 17 at 53,462,552 bp
  • A to T, chromosome 17 at 78,871,233 bp
  • G to T, chromosome 17 at 79,714,266 bp
  • G to A, chromosome 18 at 37,342,656 bp
  • G to A, chromosome 19 at 24,262,043 bp
  • A to G, chromosome 19 at 37,913,357 bp
  • A to T, chromosome 19 at 42,263,447 bp
  • A to G, chromosome 19 at 45,577,019 bp
  • G to T, chromosome X at 20,860,100 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6195 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044335-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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