Strain Name:
C57BL/6J-MtgxR6214Btlr/Mmmh
Stock Number:
044347-MU
Citation ID:
RRID:MMRRC_044347-MU
Other Names:
R6214 (G1)
Major Collection:

Strain Information

Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20269
Homologene: 56005
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56381
Homologene: 124461
Dmtn
Name: dematin actin binding protein
Synonyms: dematin, Epb4.9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 13829
VEGA: 14
HGNC: HGNC:3382
Homologene: 1496
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Dido1
Name: death inducer-obliterator 1
Synonyms: DIO-1, 6720461J16Rik, D130048F08Rik, Datf1, dido, C130092D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23856
HGNC: HGNC:2680
Homologene: 34139
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Ywhag
Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
Synonyms: D7Bwg1348e, 14-3-3 gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22628
Homologene: 22725
Myo1d
Name: myosin ID
Synonyms: 9930104H07Rik, D11Ertd9e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 338367
HGNC: HGNC:7598
Homologene: 45576
Gabbr1
Name: gamma-aminobutyric acid (GABA) B receptor, 1
Synonyms: GABAbR1, GABAB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 54393
HGNC: HGNC:4070
Homologene: 1132
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: CRP-[b], CRP-[a], ebnerin, hensin, vomeroglandin, Crpd, MUCLIN, gp300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Atp13a5
Name: ATPase type 13A5
Synonyms: C630015F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268878
Homologene: 77451
Ms4a6c
Name: membrane-spanning 4-domains, subfamily A, member 6C
Synonyms: 2200009H22Rik, 2210417N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73656
Homologene: 130765
Cntrl
Name: centriolin
Synonyms: IB3/5, 6720467O09Rik, Ma2a8, Cep1, b2b1468Clo, Cep110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26920
HGNC: HGNC:1858
Homologene: 38260
Glis2
Name: GLIS family zinc finger 2
Synonyms: Nkl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 83396
Homologene: 12821
Asap3
Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Synonyms: UPLC1, 9430088F20Rik, Ddefl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230837
Homologene: 41190
Prom2
Name: prominin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 192212
Homologene: 44536
Pde4d
Name: phosphodiesterase 4D, cAMP specific
Synonyms: dunce, Dpde3, 9630011N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238871
HGNC: HGNC:8783
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Chia1
Name: chitinase, acidic 1
Synonyms: AMCase, YNL, 2200003E03Rik, Chia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 81600
Homologene: 75168
Nav3
Name: neuron navigator 3
Synonyms: Pomfil1p, POMFIL1, 4732483H20Rik, unc53H3, steerin 3, 9630020C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 260315
Homologene: 56688
Aste1
Name: asteroid homolog 1
Synonyms: 1100001A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66595
Homologene: 130666
Ttc21a
Name: tetratricopeptide repeat domain 21A
Synonyms: 4921538N17Rik, Thm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74052
Homologene: 14728
Cd79b
Name: CD79B antigen
Synonyms: B29, Igb, Igbeta, Ig-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15985
HGNC: HGNC:1699
Homologene: 521
Flg2
Name: filaggrin family member 2
Synonyms: EG229574
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229574
Homologene: 134146
Vsig10
Name: V-set and immunoglobulin domain containing 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231668
Homologene: 10414
Ccdc13
Name: coiled-coil domain containing 13
Synonyms: 2900041A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100502861
Homologene: 87213
Ctdnep1
Name: CTD nuclear envelope phosphatase 1
Synonyms: 2610507E10Rik, Dullard
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67181
Homologene: 9100
Hipk3
Name: homeodomain interacting protein kinase 3
Synonyms: DYRK6, FIST3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15259
HGNC: HGNC:4915
Homologene: 55923
Tm6sf2
Name: transmembrane 6 superfamily member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 107770
Homologene: 77694
Lrrc9
Name: leucine rich repeat containing 9
Synonyms: 4930432K16Rik, 4921529O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 78257
Homologene: 12692
Vmn2r99
Name: vomeronasal 2, receptor 99
Synonyms: EG665376
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 665376
Homologene: 115024
Pcsk7
Name: proprotein convertase subtilisin/kexin type 7
Synonyms: SPC7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18554
VEGA: 9
HGNC: HGNC:8748
Homologene: 37955
Slc13a1
Name: solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms: NaSi-1, Nas1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 55961
Homologene: 31893
Pira13
Name: paired-Ig-like receptor A13
Synonyms: ENSMUSG00000074419, Gm15448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100041146
HGNC: HGNC:6609
Homologene: 134028
Kcnd1
Name: potassium voltage-gated channel, Shal-related family, member 1
Synonyms: Kv4.1, mShal1, Kca2-1, Shal, 1110037K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 16506
HGNC: HGNC:6237
Homologene: 21035
Glra3
Name: glycine receptor, alpha 3 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 110304
HGNC: HGNC:4328
Homologene: 142
Or8b3b
Name: olfactory receptor family 8 subfamily B member 3B
Synonyms: GA_x6K02T2PVTD-32375756-32374818, MOR164-3, Olfr918
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258372
VEGA: 9
Trim21
Name: tripartite motif-containing 21
Synonyms: Ro52, Ssa1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20821
Homologene: 2365
Gm8890
Name: predicted gene 8890
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 667948
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 35,129,634 bp
  • T to C, chromosome 2 at 65,495,036 bp
  • T to A, chromosome 2 at 76,880,208 bp
  • T to C, chromosome 2 at 104,433,741 bp
  • A to G, chromosome 2 at 127,539,775 bp
  • T to C, chromosome 2 at 180,662,152 bp
  • G to T, chromosome 3 at 93,201,859 bp
  • T to C, chromosome 3 at 106,122,445 bp
  • A to C, chromosome 4 at 136,241,425 bp
  • C to T, chromosome 4 at 138,058,003 bp
  • C to T, chromosome 4 at 141,479,112 bp
  • C to T, chromosome 5 at 11,257,230 bp
  • G to A, chromosome 5 at 117,343,924 bp
  • A to G, chromosome 5 at 135,911,074 bp
  • A to T, chromosome 6 at 24,090,796 bp
  • A to G, chromosome 7 at 3,821,718 bp
  • G to GACGGCGGCA, chromosome 7 at 97,579,909 bp
  • A to G, chromosome 7 at 102,559,439 bp
  • G to T, chromosome 7 at 131,066,733 bp
  • A to G, chromosome 8 at 55,991,256 bp
  • T to C, chromosome 8 at 70,073,074 bp
  • A to G, chromosome 9 at 38,673,214 bp
  • A to C, chromosome 9 at 45,910,376 bp
  • A to G, chromosome 9 at 105,396,857 bp
  • A to G, chromosome 9 at 119,966,772 bp
  • A to G, chromosome 9 at 121,798,909 bp
  • A to G, chromosome 10 at 109,852,565 bp
  • T to C, chromosome 11 at 69,989,508 bp
  • A to T, chromosome 11 at 80,779,791 bp
  • C to A, chromosome 11 at 106,312,441 bp
  • A to T, chromosome 12 at 72,459,853 bp
  • C to T, chromosome 13 at 109,949,433 bp
  • A to G, chromosome 14 at 70,613,336 bp
  • T to A, chromosome 16 at 4,610,333 bp
  • T to C, chromosome 16 at 29,251,407 bp
  • A to G, chromosome 17 at 19,382,558 bp
  • A to G, chromosome 17 at 37,069,365 bp
  • T to C, chromosome 19 at 11,471,136 bp
  • G to C, chromosome X at 7,823,909 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6214 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044347-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.