Strain Name:
C57BL/6J-MtgxR6216Btlr/Mmmh
Stock Number:
044349-MU
Citation ID:
RRID:MMRRC_044349-MU
Other Names:
R6216 (G1)
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: oct-1, Oct-1A, Otf-1, Oct-1C, 2810482H01Rik, Oct-1z, Oct1, Oct-1B, Otf1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Ptx3
Name: pentraxin related gene
Synonyms: pentraxin 3, TSG-14
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19288
HGNC: HGNC:9692
Homologene: 7500
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: T-type Cav3.2, alpha13.2, Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Bpifb3
Name: BPI fold containing family B, member 3
Synonyms: Rya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 378700
Homologene: 18807
Ubald2
Name: UBA-like domain containing 2
Synonyms: D030012E24Rik, Fam100b, 1110014K08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319370
Homologene: 100077
Bbx
Name: bobby sox HMG box containing
Synonyms: 5730403O13Rik, 5530401J07Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70508
Homologene: 10634
Terf1
Name: telomeric repeat binding factor 1
Synonyms: Trf1, Trbf1, Pin2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21749
Homologene: 7570
Nf1
Name: neurofibromin 1
Synonyms: Mhdadsk9, neurofibromin, Nf-1, Dsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Trip11
Name: thyroid hormone receptor interactor 11
Synonyms: GMAP-210, TRIP230, 3110031G15Rik, 6030460N08Rik, 2610511G22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 109181
Homologene: 20897
Ss18l1
Name: SS18, nBAF chromatin remodeling complex subunit like 1
Synonyms: CREST, A230053O16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269397
Homologene: 9191
Sart3
Name: squamous cell carcinoma antigen recognized by T cells 3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53890
Homologene: 40977
Arid2
Name: AT-rich interaction domain 2
Synonyms: zipzap/p200, 1700124K17Rik, 4432409D24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 77044
Homologene: 14601
Heatr1
Name: HEAT repeat containing 1
Synonyms: B130016L12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 217995
VEGA: 13
Homologene: 34562
Rsf1
Name: remodeling and spacing factor 1
Synonyms: Hbxap, p325, XAP8, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Fermt2
Name: fermitin family member 2
Synonyms: Plekhc1, Mig2, Kindlin-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218952
Homologene: 4976
Rab21
Name: RAB21, member RAS oncogene family
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216344
VEGA: 10
Homologene: 8991
Rasa2
Name: RAS p21 protein activator 2
Synonyms: 5430433H21Rik, GAP1m
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114713
HGNC: HGNC:9872
Homologene: 4745
Afg3l2
Name: AFG3-like AAA ATPase 2
Synonyms: Emv66, 2310036I02Rik, par
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 69597
VEGA: 18
HGNC: HGNC:315
Homologene: 4947
Dhx16
Name: DEAH-box helicase 16
Synonyms: DEAH (Asp-Glu-Ala-His) box polypeptide 16, DBP2, 2410006N22Rik, Ddx16
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 69192
HGNC: HGNC:2739
Homologene: 2658
Ccser2
Name: coiled-coil serine rich 2
Synonyms: 1700012P13Rik, Gcap14, 2900054P12Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 72972
Homologene: 10367
Ppp1r15a
Name: protein phosphatase 1, regulatory subunit 15A
Synonyms: Gadd34, Myd116
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17872
Homologene: 8639
Rhot1
Name: ras homolog family member T1
Synonyms: Arht1, Miro1, 2210403N23Rik, FLJ11040, C430039G08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 59040
Homologene: 56803
Mlec
Name: malectin
Synonyms: 2410014A08Rik, ESTM19
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 109154
Homologene: 8823
Paxip1
Name: PAX interacting (with transcription-activation domain) protein 1
Synonyms: D5Ertd149e, PTIP
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 55982
HGNC: HGNC:8624
Pfkp
Name: phosphofructokinase, platelet
Synonyms: 9330125N24Rik, PFK-C, 1200015H23Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56421
HGNC: HGNC:8878
Homologene: 20579
Nipbl
Name: NIPBL cohesin loading factor
Synonyms: 4933421G18Rik, 4921518A06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71175
VEGA: 15
Homologene: 15850
1700017B05Rik
Name: RIKEN cDNA 1700017B05 gene
Synonyms: D9Ertd278e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74211
VEGA: 9
Homologene: 19283
Piezo1
Name: piezo-type mechanosensitive ion channel component 1
Synonyms: Fam38a, Piezo1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234839
Homologene: 124356
Selenom
Name: selenoprotein M
Synonyms: Selm, SelM, 1500040L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 114679
Homologene: 14237
Selenop
Name: selenoprotein P
Synonyms: Se-P, D15Ucla1, selp, Sepp1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20363
Homologene: 3945
Sycp2l
Name: synaptonemal complex protein 2-like
Synonyms: EG621792, Gm40956, LOC218175
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 637277
Homologene: 52882
Myo16
Name: myosin XVI
Synonyms: BM140241, Nyap3, C230040D10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244281
Homologene: 34710
Tmem116
Name: transmembrane protein 116
Synonyms: 4930406A18Rik, 4930513P12Rik, C030022K24Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77462
Homologene: 12676
Fsd1l
Name: fibronectin type III and SPRY domain containing 1-like
Synonyms: Fsd1nl, Csdufd1, Ccdc10, A230072O16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 319636
Homologene: 45825
Galnt18
Name: polypeptide N-acetylgalactosaminyltransferase 18
Synonyms: Galntl4, 2900011G21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233733
Homologene: 18347
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Gucy1b1
Name: guanylate cyclase 1, soluble, beta 1
Synonyms: Gucy1b3, beta 1 sGC
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54195
HGNC: HGNC:4687
Homologene: 664
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mgr1, Mass1, Gpr98, VLGR1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Kng2
Name: kininogen 2
Synonyms: Kininogen-II
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 385643
HGNC: HGNC:6383
Homologene: 88343
Ppfia4
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
Synonyms: LOC100042382, Gm3812, Liprin-alpha4, 1110008G13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68507
HGNC: HGNC:9248
Homologene: 66200
Prpsap1
Name: phosphoribosyl pyrophosphate synthetase-associated protein 1
Synonyms: PAP39, 5730409F23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67763
HGNC: HGNC:9466
Homologene: 55687
Grin2b
Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2)
Synonyms: NMDAR2B, Nmdar2b, GluN2B, NR2B, GluRepsilon2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14812
HGNC: HGNC:4586
Homologene: 646
Rdh1
Name: retinol dehydrogenase 1 (all trans)
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 107605
Homologene: 129514
Hadhb
Name: hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta
Synonyms: Mtpb, 4930479F15Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231086
HGNC: HGNC:4803
Homologene: 153
Pkd1l2
Name: polycystic kidney disease 1 like 2
Synonyms: 1700126L06Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76645
Homologene: 124481
Ccdc28a
Name: coiled-coil domain containing 28A
Synonyms: 1700009P13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215814
Homologene: 56705
Hrnr
Name: hornerin
Synonyms: S100a18, A530063N20Rik, 1110033K19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68723
Homologene: 138092
Etv2
Name: ets variant 2
Synonyms: Etsrp71
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14008
HGNC: HGNC:3491
Homologene: 7308
Extl1
Name: exostosin-like glycosyltransferase 1
Synonyms: D430033M16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56219
HGNC: HGNC:3515
Homologene: 3277
Or2ag17
Name: olfactory receptor family 2 subfamily AG member 17
Synonyms: MOR283-10P, GA_x6K02T2PBJ9-9168355-9167405, Olfr699
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258180
Homologene: 79391
Pabpc2
Name: poly(A) binding protein, cytoplasmic 2
Synonyms: Pabp2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 18459
Homologene: 56509
Kiz
Name: kizuna centrosomal protein
Synonyms: LOC228730, Ncrna00153, Gm114, Plk1s1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228730
Homologene: 10219
Ddx49
Name: DEAD box helicase 49
Synonyms: R27090_2, DEAD (Asp-Glu-Ala-Asp) box polypeptide 49
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234374
Homologene: 41308
Fam163a
Name: family with sequence similarity 163, member A
Synonyms: A230106N23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329274
Homologene: 18306
Vmn2r101
Name: vomeronasal 2, receptor 101
Synonyms: EG627576
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627576
Homologene: 115024
Alkbh3
Name: alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase
Synonyms: 1700108H04Rik, mABH3, Abh3, 1810020C19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69113
Homologene: 16393
Or51a10
Name: olfactory receptor family 51 subfamily A member 10
Synonyms: GA_x6K02T2PBJ9-6784380-6783436, Olfr642, MOR13-6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258326
Homologene: 17196
Skint1
Name: selection and upkeep of intraepithelial T cells 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 639781
Homologene: 87538
Kcnd1
Name: potassium voltage-gated channel, Shal-related family, member 1
Synonyms: Kv4.1, Shal, Kca2-1, mShal1, 1110037K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 16506
HGNC: HGNC:6237
Homologene: 21035
Ube2w
Name: ubiquitin-conjugating enzyme E2W (putative)
Synonyms: 6130401J04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66799
Homologene: 10119
Bivm
Name: basic, immunoglobulin-like variable motif containing
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 246229
Homologene: 9783
Trdn
Name: triadin
Synonyms: triadin-1, EG432451, triadin 3, triadin 1, 2310045H21Rik, triadin-2, triadin-3, triadin 2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76757
VEGA: 10
Homologene: 38137
Ceacam1
Name: CEA cell adhesion molecule 1
Synonyms: MHVR1, Bgp1, Cea1, Hv2, C-CAM, Cea7, Cea-7, CD66a, Mhv-1, Cea-1, Bgp, Hv-2, mmCGM1, mCEA1, mmCGM2, Cc1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26365
Homologene: 128630
Hace1
Name: HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
Synonyms: A730034A22Rik, 1700042J16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 209462
Homologene: 10807
Or4c11c
Name: olfactory receptor family 4 subfamily C member 11C
Synonyms: MOR230-3, Olfr1205, Olfr1203, MOR230-1, GA_x6K02T2Q125-50304328-50305251, GA_x6K02T2Q125-50336588-50337313
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258898
Homologene: 81567
Mfap3l
Name: microfibrillar-associated protein 3-like
Synonyms: NYD-sp9, 5430405D20Rik, 4933428A15Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71306
Homologene: 11013
Anks1b
Name: ankyrin repeat and sterile alpha motif domain containing 1B
Synonyms: AIDA-1b, LOC380650, E530015N03Rik, Gm10937, C030032C09Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77531
Homologene: 51570
Dnaaf6rt
Name: dynein axonemal assembly factor 6, retrotransposed
Synonyms: 4930521A18Rik, Dnaaf6, Pih1d3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74708
Homologene: 16553
Ankrd22
Name: ankyrin repeat domain 22
Synonyms: 5430429D21Rik, D19Ertd675e
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 52024
Homologene: 34920
Or4a70
Name: olfactory receptor family 4 subfamily A member 70
Synonyms: MOR231-5, GA_x6K02T2Q125-50937307-50936387, Olfr1242
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258970
Homologene: 27316
Pcdhgb5
Name: protocadherin gamma subfamily B, 5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93702
HGNC: HGNC:8712
Homologene: 55773
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 15,818,997 bp
  • C to G, chromosome 1 at 16,619,284 bp
  • A to T, chromosome 1 at 31,223,351 bp
  • T to A, chromosome 1 at 44,126,868 bp
  • T to C, chromosome 1 at 134,329,183 bp
  • A to T, chromosome 1 at 156,078,995 bp
  • G to T, chromosome 1 at 165,880,320 bp
  • T to C, chromosome 2 at 52,224,552 bp
  • T to C, chromosome 2 at 88,831,311 bp
  • C to A, chromosome 2 at 89,493,722 bp
  • G to A, chromosome 2 at 94,008,536 bp
  • C to A, chromosome 2 at 146,889,497 bp
  • A to G, chromosome 2 at 153,925,853 bp
  • A to G, chromosome 2 at 180,061,913 bp
  • T to A, chromosome 3 at 66,224,844 bp
  • G to T, chromosome 3 at 82,046,713 bp
  • T to C, chromosome 3 at 93,332,162 bp
  • T to C, chromosome 4 at 53,694,742 bp
  • T to A, chromosome 4 at 112,021,482 bp
  • T to C, chromosome 4 at 134,363,130 bp
  • T to C, chromosome 5 at 27,766,173 bp
  • T to A, chromosome 5 at 30,174,931 bp
  • C to T, chromosome 5 at 113,743,206 bp
  • T to C, chromosome 5 at 115,150,317 bp
  • C to T, chromosome 5 at 121,491,108 bp
  • T to A, chromosome 6 at 135,772,399 bp
  • A to T, chromosome 7 at 25,471,996 bp
  • A to G, chromosome 7 at 30,634,611 bp
  • G to T, chromosome 7 at 45,524,022 bp
  • CG to CGACGGCGGGG, chromosome 7 at 97,579,908 bp
  • A to G, chromosome 7 at 104,049,695 bp
  • A to T, chromosome 7 at 106,790,458 bp
  • A to C, chromosome 7 at 111,513,550 bp
  • T to C, chromosome 8 at 10,315,494 bp
  • C to A, chromosome 8 at 60,671,807 bp
  • C to T, chromosome 8 at 70,297,284 bp
  • T to G, chromosome 8 at 117,081,470 bp
  • T to C, chromosome 8 at 122,489,130 bp
  • T to C, chromosome 9 at 57,257,627 bp
  • A to G, chromosome 9 at 96,544,304 bp
  • G to T, chromosome 10 at 18,224,971 bp
  • A to G, chromosome 10 at 33,305,069 bp
  • T to C, chromosome 10 at 45,618,547 bp
  • A to G, chromosome 10 at 90,260,756 bp
  • A to T, chromosome 10 at 115,294,926 bp
  • G to A, chromosome 10 at 127,764,753 bp
  • A to T, chromosome 11 at 3,514,915 bp
  • A to G, chromosome 11 at 79,411,607 bp
  • G to A, chromosome 11 at 80,251,059 bp
  • A to C, chromosome 11 at 116,434,385 bp
  • A to T, chromosome 11 at 116,471,413 bp
  • A to C, chromosome 12 at 101,890,600 bp
  • A to T, chromosome 13 at 6,619,188 bp
  • C to T, chromosome 13 at 12,432,664 bp
  • A to G, chromosome 13 at 41,141,724 bp
  • C to A, chromosome 13 at 81,524,471 bp
  • A to G, chromosome 14 at 36,940,508 bp
  • A to T, chromosome 14 at 45,459,881 bp
  • T to C, chromosome 15 at 3,279,465 bp
  • A to C, chromosome 15 at 8,318,383 bp
  • A to G, chromosome 15 at 96,356,909 bp
  • A to T, chromosome 16 at 22,987,593 bp
  • A to T, chromosome 16 at 50,251,388 bp
  • T to A, chromosome 17 at 19,591,005 bp
  • A to T, chromosome 17 at 25,378,819 bp
  • A to G, chromosome 17 at 35,882,972 bp
  • A to T, chromosome 18 at 37,731,928 bp
  • G to T, chromosome 18 at 39,774,719 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • A to T, chromosome 19 at 34,124,169 bp
  • G to C, chromosome X at 7,823,909 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6216 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044349-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.