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Strain Name:
C57BL/6J-MtgxR6216Btlr/Mmmh
Stock Number:
044349-MU
Citation ID:
RRID:MMRRC_044349-MU
Other Names:
R6216 (G1)
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Oct-1B, Oct-1A, oct-1, Otf-1, Otf1, 2810482H01Rik, Oct-1z, Oct1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Ptx3
Name: pentraxin related gene
Synonyms: TSG-14, pentraxin 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19288
HGNC: HGNC:9692
Homologene: 7500
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Bpifb3
Name: BPI fold containing family B, member 3
Synonyms: Rya3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 378700
Homologene: 18807
Ubald2
Name: UBA-like domain containing 2
Synonyms: D030012E24Rik, 1110014K08Rik, Fam100b
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319370
Homologene: 100077
Bbx
Name: bobby sox HMG box containing
Synonyms: 5730403O13Rik, 5530401J07Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70508
Homologene: 10634
Terf1
Name: telomeric repeat binding factor 1
Synonyms: Trf1, Trbf1, Pin2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21749
Homologene: 7570
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 15,818,997 bp
  • C to G, chromosome 1 at 16,619,284 bp
  • A to T, chromosome 1 at 31,223,351 bp
  • T to A, chromosome 1 at 44,126,868 bp
  • T to C, chromosome 1 at 134,329,183 bp
  • A to T, chromosome 1 at 156,078,995 bp
  • G to T, chromosome 1 at 165,880,320 bp
  • T to C, chromosome 2 at 52,224,552 bp
  • T to C, chromosome 2 at 88,831,311 bp
  • C to A, chromosome 2 at 89,493,722 bp
  • G to A, chromosome 2 at 94,008,536 bp
  • C to A, chromosome 2 at 146,889,497 bp
  • A to G, chromosome 2 at 153,925,853 bp
  • A to G, chromosome 2 at 180,061,913 bp
  • T to A, chromosome 3 at 66,224,844 bp
  • G to T, chromosome 3 at 82,046,713 bp
  • T to C, chromosome 3 at 93,332,162 bp
  • T to C, chromosome 4 at 53,694,742 bp
  • T to A, chromosome 4 at 112,021,482 bp
  • T to C, chromosome 4 at 134,363,130 bp
  • T to C, chromosome 5 at 27,766,173 bp
  • T to A, chromosome 5 at 30,174,931 bp
  • C to T, chromosome 5 at 113,743,206 bp
  • T to C, chromosome 5 at 115,150,317 bp
  • C to T, chromosome 5 at 121,491,108 bp
  • T to A, chromosome 6 at 135,772,399 bp
  • A to T, chromosome 7 at 25,471,996 bp
  • A to G, chromosome 7 at 30,634,611 bp
  • G to T, chromosome 7 at 45,524,022 bp
  • CG to CGACGGCGGGG, chromosome 7 at 97,579,908 bp
  • A to G, chromosome 7 at 104,049,695 bp
  • A to T, chromosome 7 at 106,790,458 bp
  • A to C, chromosome 7 at 111,513,550 bp
  • T to C, chromosome 8 at 10,315,494 bp
  • C to A, chromosome 8 at 60,671,807 bp
  • C to T, chromosome 8 at 70,297,284 bp
  • T to G, chromosome 8 at 117,081,470 bp
  • T to C, chromosome 8 at 122,489,130 bp
  • T to C, chromosome 9 at 57,257,627 bp
  • A to G, chromosome 9 at 96,544,304 bp
  • G to T, chromosome 10 at 18,224,971 bp
  • A to G, chromosome 10 at 33,305,069 bp
  • T to C, chromosome 10 at 45,618,547 bp
  • A to G, chromosome 10 at 90,260,756 bp
  • A to T, chromosome 10 at 115,294,926 bp
  • G to A, chromosome 10 at 127,764,753 bp
  • A to T, chromosome 11 at 3,514,915 bp
  • A to G, chromosome 11 at 79,411,607 bp
  • G to A, chromosome 11 at 80,251,059 bp
  • A to C, chromosome 11 at 116,434,385 bp
  • A to T, chromosome 11 at 116,471,413 bp
  • A to C, chromosome 12 at 101,890,600 bp
  • A to T, chromosome 13 at 6,619,188 bp
  • C to T, chromosome 13 at 12,432,664 bp
  • A to G, chromosome 13 at 41,141,724 bp
  • C to A, chromosome 13 at 81,524,471 bp
  • A to G, chromosome 14 at 36,940,508 bp
  • A to T, chromosome 14 at 45,459,881 bp
  • T to C, chromosome 15 at 3,279,465 bp
  • A to C, chromosome 15 at 8,318,383 bp
  • A to G, chromosome 15 at 96,356,909 bp
  • A to T, chromosome 16 at 22,987,593 bp
  • A to T, chromosome 16 at 50,251,388 bp
  • T to A, chromosome 17 at 19,591,005 bp
  • A to T, chromosome 17 at 25,378,819 bp
  • A to G, chromosome 17 at 35,882,972 bp
  • A to T, chromosome 18 at 37,731,928 bp
  • G to T, chromosome 18 at 39,774,719 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • A to T, chromosome 19 at 34,124,169 bp
  • G to C, chromosome X at 7,823,909 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6216 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044349-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.