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Strain Name:
C57BL/6J-MtgxR6223Btlr/Mmmh
Stock Number:
044354-MU
Citation ID:
RRID:MMRRC_044354-MU
Other Names:
R6223 (G1)
Major Collection:

Strain Information

Cdc25a
Name: cell division cycle 25A
Synonyms: D9Ertd393e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12530
HGNC: HGNC:1725
Homologene: 1355
Acadm
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11364
HGNC: HGNC:89
Homologene: 3
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Ulk2
Name: unc-51 like kinase 2
Synonyms: Unc51.2, A830085I22Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 29869
Homologene: 5891
Stub1
Name: STIP1 homology and U-Box containing protein 1
Synonyms: CHIP, 2210017D18Rik, 0610033N24Rik, 2310040B03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56424
VEGA: 17
Homologene: 4281
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Tab1
Name: TGF-beta activated kinase 1/MAP3K7 binding protein 1
Synonyms: Tak1-binding protein 1, 2310012M03Rik, Map3k7ip1, b2b449Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66513
VEGA: 15
Homologene: 4461
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 87,185,412 bp
  • G to A, chromosome 1 at 88,503,442 bp
  • A to T, chromosome 1 at 127,382,979 bp
  • T to A, chromosome 1 at 133,070,357 bp
  • C to T, chromosome 2 at 30,407,030 bp
  • T to C, chromosome 2 at 62,598,259 bp
  • A to G, chromosome 2 at 104,787,648 bp
  • C to T, chromosome 2 at 125,412,671 bp
  • T to C, chromosome 2 at 144,823,948 bp
  • A to C, chromosome 2 at 152,427,953 bp
  • T to C, chromosome 2 at 181,659,057 bp
  • T to A, chromosome 3 at 10,015,110 bp
  • A to G, chromosome 3 at 15,379,026 bp
  • G to A, chromosome 3 at 103,736,460 bp
  • C to A, chromosome 3 at 153,938,549 bp
  • T to C, chromosome 4 at 48,468,525 bp
  • T to A, chromosome 4 at 126,586,168 bp
  • T to C, chromosome 4 at 143,142,207 bp
  • C to A, chromosome 4 at 148,933,383 bp
  • A to G, chromosome 4 at 154,871,702 bp
  • G to A, chromosome 5 at 91,957,967 bp
  • A to G, chromosome 5 at 137,605,299 bp
  • T to C, chromosome 6 at 72,429,605 bp
  • G to T, chromosome 6 at 112,376,448 bp
  • T to A, chromosome 6 at 124,574,580 bp
  • A to T, chromosome 7 at 8,483,302 bp
  • T to C, chromosome 7 at 19,143,209 bp
  • T to G, chromosome 7 at 27,516,836 bp
  • G to A, chromosome 7 at 81,473,462 bp
  • A to G, chromosome 7 at 89,956,287 bp
  • A to G, chromosome 7 at 101,579,951 bp
  • C to T, chromosome 7 at 125,676,625 bp
  • C to A, chromosome 8 at 22,940,426 bp
  • A to C, chromosome 8 at 70,109,954 bp
  • C to A, chromosome 9 at 109,889,774 bp
  • G to A, chromosome 9 at 121,763,787 bp
  • A to G, chromosome 9 at 121,798,909 bp
  • A to T, chromosome 10 at 34,395,187 bp
  • A to T, chromosome 11 at 61,787,504 bp
  • A to G, chromosome 11 at 62,552,525 bp
  • G to T, chromosome 11 at 67,098,017 bp
  • G to T, chromosome 11 at 107,171,616 bp
  • A to G, chromosome 11 at 109,977,846 bp
  • A to G, chromosome 12 at 100,387,109 bp
  • T to C, chromosome 12 at 116,257,458 bp
  • G to A, chromosome 13 at 21,611,366 bp
  • G to A, chromosome 14 at 21,845,178 bp
  • A to G, chromosome 14 at 52,450,679 bp
  • T to C, chromosome 14 at 93,015,733 bp
  • T to A, chromosome 15 at 66,707,922 bp
  • T to A, chromosome 15 at 80,148,263 bp
  • A to T, chromosome 15 at 82,791,717 bp
  • A to T, chromosome 16 at 17,357,571 bp
  • C to T, chromosome 16 at 91,387,988 bp
  • C to T, chromosome 17 at 25,832,813 bp
  • C to A, chromosome 17 at 29,327,439 bp
  • T to C, chromosome 17 at 65,500,196 bp
  • A to C, chromosome 18 at 67,358,739 bp
  • A to T, chromosome 19 at 25,161,052 bp
  • G to A, chromosome 19 at 41,135,952 bp
  • T to C, chromosome 19 at 56,865,850 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6223 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044354-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.