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Strain Name:
C57BL/6J-MtgxR6233Btlr/Mmmh
Stock Number:
044361-MU
Citation ID:
RRID:MMRRC_044361-MU
Other Names:
R6233 (G1)
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Rbm25
Name: RNA binding motif protein 25
Synonyms: 2610015J01Rik, A130095G20Rik, 2600011C06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67039
Fbxo28
Name: F-box protein 28
Synonyms: 5730505P19Rik, 4833428J17Rik, Fbx28, D1Ertd578e
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67948
Homologene: 9050
Eefsec
Name: eukaryotic elongation factor, selenocysteine-tRNA-specific
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 65967
Homologene: 11073
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,375,286 bp
  • G to A, chromosome 1 at 46,204,269 bp
  • A to T, chromosome 1 at 153,223,666 bp
  • A to G, chromosome 1 at 170,908,889 bp
  • A to G, chromosome 1 at 182,341,508 bp
  • T to A, chromosome 2 at 58,448,399 bp
  • T to A, chromosome 2 at 59,907,511 bp
  • T to A, chromosome 2 at 66,277,618 bp
  • T to A, chromosome 2 at 76,737,653 bp
  • T to C, chromosome 2 at 86,413,207 bp
  • T to C, chromosome 2 at 87,868,560 bp
  • T to A, chromosome 2 at 90,813,313 bp
  • A to T, chromosome 2 at 120,392,175 bp
  • T to C, chromosome 2 at 151,653,542 bp
  • C to T, chromosome 2 at 155,631,799 bp
  • A to G, chromosome 2 at 174,646,697 bp
  • A to G, chromosome 3 at 57,832,970 bp
  • A to G, chromosome 3 at 80,707,203 bp
  • T to A, chromosome 4 at 4,069,984 bp
  • T to A, chromosome 4 at 59,076,245 bp
  • A to G, chromosome 4 at 122,999,920 bp
  • T to A, chromosome 4 at 134,926,802 bp
  • T to C, chromosome 5 at 43,979,362 bp
  • T to C, chromosome 5 at 114,767,114 bp
  • T to C, chromosome 5 at 137,823,501 bp
  • A to G, chromosome 5 at 140,760,692 bp
  • G to T, chromosome 6 at 5,077,610 bp
  • A to G, chromosome 6 at 48,930,965 bp
  • A to G, chromosome 6 at 88,358,544 bp
  • T to C, chromosome 6 at 127,938,264 bp
  • T to C, chromosome 7 at 13,832,675 bp
  • T to C, chromosome 7 at 28,131,460 bp
  • C to A, chromosome 7 at 30,779,679 bp
  • A to T, chromosome 7 at 64,354,371 bp
  • G to A, chromosome 7 at 85,869,891 bp
  • A to T, chromosome 7 at 104,179,754 bp
  • T to G, chromosome 8 at 43,625,912 bp
  • A to C, chromosome 8 at 48,417,059 bp
  • A to G, chromosome 8 at 72,762,885 bp
  • A to T, chromosome 8 at 80,879,532 bp
  • A to G, chromosome 8 at 84,588,753 bp
  • T to G, chromosome 9 at 37,420,929 bp
  • T to A, chromosome 10 at 45,670,443 bp
  • T to C, chromosome 10 at 58,270,984 bp
  • G to A, chromosome 10 at 93,850,340 bp
  • T to C, chromosome 10 at 129,647,296 bp
  • A to G, chromosome 11 at 31,666,740 bp
  • T to A, chromosome 11 at 58,997,207 bp
  • A to G, chromosome 11 at 79,565,975 bp
  • G to A, chromosome 11 at 99,543,094 bp
  • A to T, chromosome 11 at 103,613,388 bp
  • T to A, chromosome 11 at 119,210,565 bp
  • T to C, chromosome 12 at 72,619,567 bp
  • G to A, chromosome 12 at 83,659,426 bp
  • A to C, chromosome 12 at 104,785,455 bp
  • A to T, chromosome 12 at 110,977,120 bp
  • G to A, chromosome 12 at 117,739,718 bp
  • T to C, chromosome 13 at 73,805,471 bp
  • T to A, chromosome 13 at 112,964,537 bp
  • T to G, chromosome 14 at 43,544,631 bp
  • G to A, chromosome 14 at 60,692,007 bp
  • T to A, chromosome 14 at 60,872,105 bp
  • T to C, chromosome 14 at 88,468,052 bp
  • A to G, chromosome 15 at 44,433,660 bp
  • A to G, chromosome 16 at 26,710,506 bp
  • T to A, chromosome 16 at 56,778,642 bp
  • A to C, chromosome 16 at 62,898,921 bp
  • T to C, chromosome 17 at 27,086,960 bp
  • CCACAC to CC, chromosome 17 at 32,775,520 bp
  • T to C, chromosome 17 at 33,919,982 bp
  • A to G, chromosome 17 at 43,629,752 bp
  • A to G, chromosome 17 at 53,462,552 bp
  • A to T, chromosome 17 at 78,871,233 bp
  • G to T, chromosome 17 at 79,714,266 bp
  • C to T, chromosome 18 at 19,965,795 bp
  • A to G, chromosome 18 at 35,624,444 bp
  • T to C, chromosome 19 at 6,928,329 bp
  • T to C, chromosome 19 at 13,471,740 bp
  • G to A, chromosome 19 at 46,475,632 bp
  • C to T, chromosome X at 74,277,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6233 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044361-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.