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Strain Name:
C57BL/6J-MtgxR6247Btlr/Mmmh
Stock Number:
044366-MU
Citation ID:
RRID:MMRRC_044366-MU
Other Names:
R6247 (G1)
Major Collection:

Strain Information

Cdk6
Name: cyclin dependent kinase 6
Synonyms: Crk2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12571
HGNC: HGNC:1777
Homologene: 963
Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Wasf1
Name: WASP family, member 1
Synonyms: WAVE-1, Scar, WAVE
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 83767
Homologene: 2920
Psme4
Name: proteasome (prosome, macropain) activator subunit 4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103554
Homologene: 113742
Tmem104
Name: transmembrane protein 104
Synonyms: C630005D06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 320534
Homologene: 9802
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 36,163,228 bp
  • A to G, chromosome 1 at 46,225,888 bp
  • A to G, chromosome 1 at 74,432,339 bp
  • A to T, chromosome 1 at 92,012,838 bp
  • G to T, chromosome 1 at 107,082,760 bp
  • T to C, chromosome 1 at 118,305,209 bp
  • C to A, chromosome 1 at 130,743,043 bp
  • T to C, chromosome 2 at 25,621,681 bp
  • T to C, chromosome 2 at 73,707,006 bp
  • T to A, chromosome 2 at 83,879,208 bp
  • A to T, chromosome 2 at 126,775,793 bp
  • A to G, chromosome 2 at 160,950,048 bp
  • C to T, chromosome 2 at 177,193,724 bp
  • A to T, chromosome 3 at 62,380,960 bp
  • C to G, chromosome 3 at 93,398,130 bp
  • T to C, chromosome 4 at 34,692,530 bp
  • T to C, chromosome 4 at 41,605,775 bp
  • A to T, chromosome 4 at 76,066,291 bp
  • G to A, chromosome 4 at 88,407,140 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • T to A, chromosome 4 at 115,884,790 bp
  • C to T, chromosome 4 at 129,686,791 bp
  • C to T, chromosome 4 at 130,172,932 bp
  • G to A, chromosome 5 at 3,344,553 bp
  • A to G, chromosome 5 at 73,065,481 bp
  • A to T, chromosome 5 at 123,158,398 bp
  • T to A, chromosome 5 at 124,546,992 bp
  • C to A, chromosome 5 at 139,813,072 bp
  • T to C, chromosome 6 at 72,604,935 bp
  • T to C, chromosome 6 at 84,066,999 bp
  • C to A, chromosome 6 at 88,849,317 bp
  • T to A, chromosome 6 at 122,880,652 bp
  • T to C, chromosome 6 at 122,928,042 bp
  • T to C, chromosome 7 at 6,353,946 bp
  • G to T, chromosome 7 at 24,105,105 bp
  • T to C, chromosome 7 at 28,888,652 bp
  • G to T, chromosome 7 at 35,186,587 bp
  • G to A, chromosome 7 at 51,566,131 bp
  • A to G, chromosome 7 at 101,253,540 bp
  • C to T, chromosome 7 at 107,785,441 bp
  • A to T, chromosome 7 at 109,898,682 bp
  • T to C, chromosome 7 at 128,185,787 bp
  • A to G, chromosome 8 at 16,196,235 bp
  • TCGACG to TCG, chromosome 8 at 71,890,708 bp
  • A to G, chromosome 9 at 7,135,078 bp
  • T to A, chromosome 9 at 15,862,786 bp
  • A to T, chromosome 9 at 53,545,238 bp
  • C to T, chromosome 9 at 72,726,438 bp
  • G to C, chromosome 9 at 108,981,062 bp
  • A to G, chromosome 9 at 110,488,544 bp
  • G to T, chromosome 10 at 5,349,071 bp
  • T to A, chromosome 10 at 40,937,745 bp
  • A to G, chromosome 10 at 44,446,786 bp
  • G to T, chromosome 10 at 86,776,663 bp
  • T to C, chromosome 11 at 9,403,874 bp
  • A to T, chromosome 11 at 28,339,706 bp
  • T to C, chromosome 11 at 30,853,245 bp
  • C to T, chromosome 11 at 48,915,908 bp
  • T to C, chromosome 11 at 72,158,942 bp
  • C to T, chromosome 11 at 100,320,397 bp
  • A to G, chromosome 11 at 115,243,993 bp
  • G to A, chromosome 12 at 8,001,801 bp
  • G to A, chromosome 12 at 16,716,675 bp
  • T to C, chromosome 12 at 56,709,695 bp
  • A to G, chromosome 12 at 79,282,984 bp
  • G to A, chromosome 12 at 86,758,556 bp
  • A to G, chromosome 12 at 112,628,356 bp
  • A to T, chromosome 13 at 14,234,425 bp
  • T to C, chromosome 13 at 42,157,490 bp
  • C to T, chromosome 13 at 45,408,481 bp
  • T to C, chromosome 13 at 55,625,173 bp
  • A to G, chromosome 13 at 67,140,460 bp
  • C to T, chromosome 14 at 55,636,370 bp
  • T to C, chromosome 14 at 55,762,651 bp
  • C to A, chromosome 14 at 60,378,872 bp
  • G to T, chromosome 14 at 75,343,736 bp
  • A to G, chromosome 15 at 4,763,541 bp
  • T to A, chromosome 15 at 78,539,588 bp
  • G to T, chromosome 15 at 83,227,819 bp
  • A to T, chromosome 15 at 99,745,924 bp
  • A to T, chromosome 16 at 73,967,784 bp
  • G to A, chromosome 17 at 18,022,486 bp
  • C to A, chromosome 17 at 26,962,957 bp
  • C to T, chromosome 17 at 35,961,064 bp
  • A to G, chromosome 18 at 36,654,146 bp
  • T to C, chromosome 19 at 8,930,949 bp
  • A to G, chromosome 19 at 38,745,845 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6247 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044366-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.