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Strain Name:
C57BL/6J-MtgxR6304Btlr/Mmmh
Stock Number:
044380-MU
Citation ID:
RRID:MMRRC_044380-MU
Other Names:
R6304 (G1)
Major Collection:

Strain Information

Nrp2
Name: neuropilin 2
Synonyms: NP2, Npn-2, Npn2, NP-2, 1110048P06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18187
HGNC: HGNC:8005
Homologene: 2875
Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Etaa1
Name: Ewing tumor-associated antigen 1
Synonyms: 5730466H23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68145
Homologene: 10369
Kdm2b
Name: lysine (K)-specific demethylase 2B
Synonyms: Cxxc2, Jhdm1b, Fbxl10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 30841
Homologene: 13069
Plcg1
Name: phospholipase C, gamma 1
Synonyms: Plc-1, Plcg-1, Plc-gamma1, Cded
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18803
HGNC: HGNC:9065
Homologene: 1997
Sh3gl1
Name: SH3-domain GRB2-like 1
Synonyms: SH3P8, EEN, endophilin II, Sh3d2b, endophilin A2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20405
VEGA: 17
Homologene: 55709
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 62,745,406 bp
  • T to A, chromosome 2 at 32,250,790 bp
  • A to T, chromosome 2 at 62,764,616 bp
  • A to T, chromosome 2 at 76,891,099 bp
  • G to T, chromosome 2 at 76,915,735 bp
  • T to C, chromosome 2 at 157,040,764 bp
  • A to G, chromosome 2 at 160,761,463 bp
  • G to A, chromosome 3 at 94,403,206 bp
  • A to C, chromosome 3 at 108,461,256 bp
  • T to A, chromosome 4 at 3,184,975 bp
  • C to A, chromosome 4 at 46,522,118 bp
  • T to C, chromosome 4 at 58,487,013 bp
  • T to C, chromosome 4 at 59,960,084 bp
  • T to C, chromosome 4 at 88,835,910 bp
  • T to C, chromosome 4 at 103,087,356 bp
  • T to C, chromosome 4 at 147,863,083 bp
  • C to T, chromosome 4 at 149,906,731 bp
  • A to T, chromosome 5 at 14,677,893 bp
  • A to T, chromosome 5 at 105,608,609 bp
  • A to G, chromosome 5 at 122,881,744 bp
  • A to T, chromosome 6 at 22,016,923 bp
  • A to G, chromosome 6 at 50,312,674 bp
  • T to C, chromosome 7 at 10,098,237 bp
  • G to A, chromosome 7 at 104,012,031 bp
  • A to T, chromosome 8 at 16,058,674 bp
  • A to T, chromosome 9 at 14,322,561 bp
  • C to T, chromosome 9 at 27,342,575 bp
  • A to G, chromosome 10 at 70,559,557 bp
  • T to A, chromosome 10 at 82,290,368 bp
  • A to C, chromosome 11 at 17,947,505 bp
  • G to T, chromosome 11 at 69,404,258 bp
  • A to G, chromosome 11 at 101,367,909 bp
  • A to G, chromosome 12 at 31,494,589 bp
  • C to T, chromosome 13 at 100,223,166 bp
  • T to C, chromosome 14 at 20,560,968 bp
  • A to G, chromosome 14 at 70,137,176 bp
  • T to C, chromosome 15 at 8,118,042 bp
  • T to A, chromosome 15 at 77,735,304 bp
  • A to G, chromosome 15 at 86,134,919 bp
  • T to C, chromosome 15 at 99,227,038 bp
  • T to A, chromosome 16 at 73,958,308 bp
  • A to T, chromosome 17 at 56,036,431 bp
  • T to C, chromosome 18 at 14,807,355 bp
  • C to T, chromosome 18 at 37,335,921 bp
  • T to A, chromosome 18 at 37,401,367 bp
  • T to C, chromosome 19 at 10,456,978 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6304 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044380-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.