Strain Name:
C57BL/6J-MtgxR6447Btlr/Mmmh
Stock Number:
044389-MU
Citation ID:
RRID:MMRRC_044389-MU
Other Names:
R6447 (G1)
Major Collection:

Gene Information

Ect2
Name: ect2 oncogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13605
HGNC: HGNC:3155
Homologene: 7298
Hdac2
Name: histone deacetylase 2
Synonyms: Yy1bp, D10Wsu179e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15182
HGNC: HGNC:4853
Homologene: 68187
Casr
Name: calcium-sensing receptor
Synonyms: CaR, Gprc2a, cation sensing receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12374
HGNC: HGNC:1514
Homologene: 332
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, Coh1, 2310042E16Rik, C330002D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Tbc1d1
Name: TBC1 domain family, member 1
Synonyms: 1110062G02Rik, Nob1, Nobq1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57915
Homologene: 56856
Thap1
Name: THAP domain containing, apoptosis associated protein 1
Synonyms: 4833431A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 73754
Homologene: 10005
Stau2
Name: staufen double-stranded RNA binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 29819
Homologene: 8666
Samd8
Name: sterile alpha motif domain containing 8
Synonyms: 1700010P07Rik, 1110053F04Rik, Smsr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67630
Homologene: 41670
Exoc1
Name: exocyst complex component 1
Synonyms: Sec3p, SEC3, A730011E05Rik, 2810407P21Rik, Sec3l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69940
Homologene: 41241
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Lyplal1
Name: lysophospholipase-like 1
Synonyms: Q96AVO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226791
Homologene: 13683
Pcdhb22
Name: protocadherin beta 22
Synonyms: Pcdhb15, PcdhbV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93893
HGNC: HGNC:8686
Homologene: 113752
T
Name: brachyury, T-box transcription factor T
Synonyms: T1, Bra, Tbxt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20997
Homologene: 2393
Lrrk1
Name: leucine-rich repeat kinase 1
Synonyms: D130026O16Rik, C230002E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233328
Homologene: 23464
Nwd2
Name: NACHT and WD repeat domain containing 2
Synonyms: B830017A01Rik, 3110047P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319807
Homologene: 14974
Tmprss11f
Name: transmembrane protease, serine 11f
Synonyms: 4732406D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243083
Homologene: 65356
Axl
Name: AXL receptor tyrosine kinase
Synonyms: Tyro7, Ark, Ufo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26362
HGNC: HGNC:905
Homologene: 7583
Smarcal1
Name: SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
Synonyms: Mharp, 6030401P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 54380
Homologene: 8558
Cntn6
Name: contactin 6
Synonyms: NB-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 53870
HGNC: HGNC:2176
Homologene: 8702
Sccpdh
Name: saccharopine dehydrogenase (putative)
Synonyms: C330023F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 109232
Homologene: 9334
Zswim2
Name: zinc finger SWIM-type containing 2
Synonyms: 1700025P14Rik, 4933437F18Rik, MEX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71861
Homologene: 32689
Vmn1r205
Name: vomeronasal 1 receptor 205
Synonyms: V1rh8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171251
Homologene: 110880
Aadacl2fm3
Name: AADACL2 family member 3
Synonyms: Gm8298
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 666803
Homologene: 86204
Eif1ad10
Name: eukaryotic translation initiation factor 1A domain containing 10
Synonyms: Gm8332
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 666862
VEGA: 12
Trdv4
Name: T cell receptor delta variable 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100114901
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 16,389,825 bp
  • C to T, chromosome 1 at 72,585,874 bp
  • A to G, chromosome 1 at 179,678,888 bp
  • A to T, chromosome 1 at 186,089,442 bp
  • T to C, chromosome 2 at 83,915,113 bp
  • A to G, chromosome 3 at 27,115,484 bp
  • A to G, chromosome 3 at 59,865,398 bp
  • T to A, chromosome 5 at 63,807,555 bp
  • C to T, chromosome 5 at 64,333,493 bp
  • T to A, chromosome 5 at 76,543,517 bp
  • T to C, chromosome 5 at 86,528,227 bp
  • A to T, chromosome 6 at 104,859,448 bp
  • C to A, chromosome 7 at 25,770,283 bp
  • A to G, chromosome 7 at 66,302,728 bp
  • G to A, chromosome 7 at 119,923,054 bp
  • T to C, chromosome 8 at 15,910,527 bp
  • CAGCATCTGCTCGGAGCA to CAGCA, chromosome 8 at 26,160,856 bp
  • T to C, chromosome 10 at 36,993,816 bp
  • T to C, chromosome 12 at 88,249,724 bp
  • G to T, chromosome 13 at 22,592,742 bp
  • T to C, chromosome 14 at 21,792,556 bp
  • A to G, chromosome 14 at 54,075,474 bp
  • T to C, chromosome 15 at 35,572,126 bp
  • A to G, chromosome 16 at 36,495,545 bp
  • T to C, chromosome 17 at 8,441,631 bp
  • A to G, chromosome 18 at 37,520,216 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6447 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044389-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.