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Strain Name:
C57BL/6J-MtgxR6296Btlr/Mmmh
Stock Number:
044407-MU
Citation ID:
RRID:MMRRC_044407-MU
Other Names:
R6296 (G1)
Major Collection:

Strain Information

Ighm
Name: immunoglobulin heavy constant mu
Synonyms: Ig mu, Igh6, Igh-M, muH, IgM
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16019
HGNC: HGNC:5541
Aatf
Name: apoptosis antagonizing transcription factor
Synonyms: 4933415H02Rik, Trb, 5830465M17Rik, Che-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56321
Homologene: 40811
Rgs9
Name: regulator of G-protein signaling 9
Synonyms: Rgs9-2, RGS9-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19739
Homologene: 2845
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Kcnc1
Name: potassium voltage gated channel, Shaw-related subfamily, member 1
Synonyms: Kv3.1, KV4, NGK2, KShIIIB, Kcr2-1, Shaw, C230009H10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16502
HGNC: HGNC:6233
Homologene: 68134
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 3,216,570 bp
  • T to A, chromosome 1 at 33,769,817 bp
  • A to G, chromosome 1 at 37,428,347 bp
  • T to A, chromosome 1 at 56,637,192 bp
  • T to A, chromosome 1 at 65,262,953 bp
  • T to G, chromosome 1 at 72,643,258 bp
  • C to A, chromosome 1 at 89,145,489 bp
  • T to C, chromosome 1 at 134,361,611 bp
  • C to T, chromosome 1 at 136,221,071 bp
  • T to C, chromosome 1 at 168,183,615 bp
  • G to A, chromosome 2 at 76,749,329 bp
  • G to A, chromosome 2 at 89,630,631 bp
  • T to G, chromosome 2 at 103,970,601 bp
  • T to A, chromosome 2 at 129,810,888 bp
  • G to C, chromosome 2 at 153,906,892 bp
  • A to C, chromosome 3 at 79,667,848 bp
  • G to T, chromosome 4 at 3,904,499 bp
  • G to T, chromosome 4 at 25,270,572 bp
  • A to T, chromosome 4 at 123,432,875 bp
  • G to A, chromosome 4 at 139,318,221 bp
  • T to A, chromosome 4 at 145,538,103 bp
  • T to C, chromosome 5 at 25,186,165 bp
  • G to A, chromosome 5 at 49,960,847 bp
  • A to C, chromosome 5 at 142,733,576 bp
  • A to T, chromosome 5 at 151,062,673 bp
  • G to T, chromosome 6 at 69,235,799 bp
  • T to C, chromosome 6 at 118,598,723 bp
  • T to A, chromosome 6 at 118,652,714 bp
  • A to G, chromosome 6 at 127,711,804 bp
  • C to A, chromosome 6 at 134,720,493 bp
  • G to A, chromosome 7 at 5,090,123 bp
  • C to T, chromosome 7 at 30,658,976 bp
  • G to A, chromosome 7 at 46,435,316 bp
  • A to G, chromosome 7 at 110,628,354 bp
  • C to T, chromosome 8 at 61,072,309 bp
  • C to T, chromosome 8 at 63,927,793 bp
  • A to G, chromosome 9 at 45,867,821 bp
  • T to A, chromosome 9 at 76,996,935 bp
  • A to T, chromosome 9 at 110,393,826 bp
  • T to C, chromosome 10 at 76,611,049 bp
  • T to C, chromosome 10 at 93,066,846 bp
  • T to A, chromosome 10 at 102,545,753 bp
  • C to T, chromosome 10 at 120,075,464 bp
  • C to A, chromosome 11 at 52,144,596 bp
  • T to C, chromosome 11 at 72,195,754 bp
  • C to T, chromosome 11 at 78,226,059 bp
  • T to C, chromosome 11 at 79,690,829 bp
  • T to C, chromosome 11 at 84,473,100 bp
  • C to T, chromosome 11 at 89,040,749 bp
  • T to A, chromosome 11 at 95,364,145 bp
  • T to A, chromosome 11 at 99,665,419 bp
  • A to T, chromosome 11 at 101,273,998 bp
  • T to C, chromosome 11 at 107,226,266 bp
  • T to C, chromosome 11 at 109,268,987 bp
  • C to T, chromosome 11 at 115,124,369 bp
  • A to G, chromosome 12 at 31,487,508 bp
  • T to C, chromosome 12 at 113,421,567 bp
  • T to A, chromosome 13 at 44,903,063 bp
  • C to T, chromosome 13 at 54,093,880 bp
  • G to A, chromosome 13 at 59,728,464 bp
  • T to C, chromosome 13 at 59,742,683 bp
  • A to C, chromosome 13 at 71,959,668 bp
  • G to C, chromosome 14 at 21,985,800 bp
  • A to T, chromosome 14 at 28,080,155 bp
  • G to T, chromosome 14 at 32,526,403 bp
  • T to A, chromosome 15 at 8,153,155 bp
  • T to C, chromosome 15 at 8,300,895 bp
  • T to C, chromosome 15 at 82,223,902 bp
  • A to G, chromosome 16 at 35,303,710 bp
  • T to C, chromosome 16 at 59,361,406 bp
  • T to C, chromosome 16 at 89,141,082 bp
  • T to C, chromosome 17 at 25,231,865 bp
  • G to A, chromosome 17 at 25,383,079 bp
  • A to G, chromosome 17 at 25,834,969 bp
  • G to T, chromosome 17 at 44,102,480 bp
  • G to A, chromosome 17 at 66,150,729 bp
  • A to T, chromosome 18 at 7,000,591 bp
  • A to G, chromosome 18 at 12,819,503 bp
  • T to C, chromosome 18 at 44,014,757 bp
  • T to G, chromosome 19 at 9,003,305 bp
  • T to C, chromosome 19 at 33,780,337 bp
  • T to G, chromosome 19 at 39,330,261 bp
  • C to T, chromosome X at 147,480,557 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6296 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044407-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.