Strain Name:
C57BL/6J-MtgxR6421Btlr
Stock Number:
044420-MU
Citation ID:
RRID:MMRRC_044420-MU
Other Names:
R6421 (G1)
Major Collection:

Gene Information

Xpo1
Name: exportin 1
Synonyms: Crm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103573
Homologene: 2554
Wwc2
Name: WW, C2 and coiled-coil domain containing 2
Synonyms: D8Ertd594e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52357
Homologene: 32618
Arel1
Name: apoptosis resistant E3 ubiquitin protein ligase 1
Synonyms: 1110018G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68497
Homologene: 8885
Ptprj
Name: protein tyrosine phosphatase, receptor type, J
Synonyms: Byp, DEP-1, Scc-1, Scc1, CD148, RPTPJ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19271
HGNC: HGNC:9673
Homologene: 2130
Frem1
Name: Fras1 related extracellular matrix protein 1
Synonyms: eyem02Jus, heb, QBRICK, eyes2, crf11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329872
Homologene: 27049
Grin2c
Name: glutamate receptor, ionotropic, NMDA2C (epsilon 3)
Synonyms: NMDAR2C, NR2C, GluRepsilon3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14813
HGNC: HGNC:4587
Homologene: 647
Stx4a
Name: syntaxin 4A (placental)
Synonyms: Stx4, Syn4, Syn-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20909
Homologene: 105435
Dcbld1
Name: discoidin, CUB and LCCL domain containing 1
Synonyms: 4631413K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66686
Homologene: 12010
St3gal1
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 1
Synonyms: CMP-N-acetylneuraminate: [beta-galactosidase alpha-2,3] sialytransferase, Siat4, ST3GalI, Siat4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20442
VEGA: 15
Homologene: 7540
Peg10
Name: paternally expressed 10
Synonyms: MEF3L, HB-1, MyEF-3, MyEF-3 like, Edr, Mart2, Mar2, Rtl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 170676
Homologene: 116067
Scn1a
Name: sodium channel, voltage-gated, type I, alpha
Synonyms: Nav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20265
Homologene: 21375
Adgrf4
Name: adhesion G protein-coupled receptor F4
Synonyms: 4632435A09Rik, Gpr115
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78249
Homologene: 51953
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Anapc15-ps
Name: anaphase promoting complex C subunit 15, pseudogene
Synonyms: Gm6843
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 628119
VEGA: 10
Myo3b
Name: myosin IIIB
Synonyms: A430065P19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329421
Homologene: 51393
Serpinb9f
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9f
Synonyms: NK21, ovalbumin, Spi13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20709
HGNC: HGNC:8955
Homologene: 69093
Zfp616
Name: zinc finger protein 616
Synonyms: Gm12330
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327963
Homologene: 88945
Traf3ip2
Name: TRAF3 interacting protein 2
Synonyms: Act1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103213
HGNC: HGNC:1343
Homologene: 15885
Lrriq4
Name: leucine-rich repeats and IQ motif containing 4
Synonyms: 4930558O21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68307
Homologene: 12260
Gm8765
Name: predicted gene 8765
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 667693
Homologene: 134512
Olfr782
Name: olfactory receptor 782
Synonyms: GA_x6K02T2PULF-11031172-11032116, MOR110-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 257985
Homologene: 133581
Gm7276
Name: predicted gene 7276
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 108168393
VEGA: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 40,889,270 bp
  • T to G, chromosome 2 at 66,272,927 bp
  • A to G, chromosome 2 at 70,313,356 bp
  • A to G, chromosome 2 at 90,471,140 bp
  • T to C, chromosome 3 at 30,650,402 bp
  • G to A, chromosome 4 at 82,994,128 bp
  • GAT to GATCAT, chromosome 6 at 4,756,449 bp
  • C to A, chromosome 7 at 127,846,501 bp
  • A to T, chromosome 8 at 47,900,746 bp
  • T to C, chromosome 10 at 39,639,404 bp
  • T to C, chromosome 10 at 52,286,354 bp
  • T to C, chromosome 10 at 95,673,214 bp
  • A to T, chromosome 10 at 129,351,501 bp
  • A to G, chromosome 11 at 23,291,490 bp
  • A to G, chromosome 11 at 74,083,870 bp
  • A to C, chromosome 11 at 115,251,130 bp
  • T to C, chromosome 12 at 84,934,345 bp
  • A to C, chromosome 13 at 33,334,533 bp
  • A to G, chromosome 13 at 50,701,951 bp
  • A to G, chromosome 13 at 81,508,736 bp
  • A to G, chromosome 15 at 67,111,346 bp
  • A to T, chromosome 17 at 42,672,501 bp
  • T to C, chromosome 18 at 77,185,670 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6421 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044420-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.