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Strain Name:
C57BL/6J-MtgxR6119Btlr/Mmmh
Stock Number:
044429-MU
Citation ID:
RRID:MMRRC_044429-MU
Other Names:
R6119 (G1)
Major Collection:

Strain Information

Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194
Prss12
Name: serine protease 12 neurotrypsin (motopsin)
Synonyms: Bssp-3, motopsin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19142
HGNC: HGNC:9477
Homologene: 7490
Gtf2i
Name: general transcription factor II I
Synonyms: TFII-I, BAP-135, 6030441I21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14886
HGNC: HGNC:4659
Homologene: 7748
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: Mthfd, DCS, E430024A07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Msra
Name: methionine sulfoxide reductase A
Synonyms: MSR-A, 2310045J23Rik, 6530413P12Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110265
HGNC: HGNC:7377
Homologene: 5812
Tnks2
Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
Synonyms: 5430432P15Rik, Parp5b
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74493
Homologene: 11890
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 2 at 5,021,323 bp
  • T to G, chromosome 2 at 25,305,158 bp
  • A to G, chromosome 2 at 52,220,931 bp
  • A to T, chromosome 2 at 87,435,389 bp
  • A to G, chromosome 2 at 88,683,591 bp
  • A to T, chromosome 2 at 103,702,310 bp
  • C to A, chromosome 2 at 113,834,350 bp
  • A to G, chromosome 2 at 158,751,127 bp
  • T to A, chromosome 3 at 58,460,253 bp
  • A to T, chromosome 3 at 83,898,382 bp
  • A to G, chromosome 3 at 123,489,609 bp
  • A to T, chromosome 3 at 129,736,772 bp
  • T to A, chromosome 4 at 138,288,332 bp
  • A to G, chromosome 5 at 14,677,019 bp
  • A to T, chromosome 5 at 134,287,057 bp
  • A to G, chromosome 5 at 135,353,420 bp
  • T to C, chromosome 6 at 48,658,954 bp
  • A to G, chromosome 6 at 57,114,233 bp
  • T to A, chromosome 6 at 113,394,741 bp
  • T to C, chromosome 6 at 135,023,355 bp
  • T to C, chromosome 7 at 4,028,896 bp
  • C to T, chromosome 7 at 27,565,713 bp
  • T to C, chromosome 7 at 80,238,992 bp
  • A to G, chromosome 7 at 105,709,440 bp
  • T to C, chromosome 7 at 126,778,365 bp
  • A to G, chromosome 8 at 14,033,692 bp
  • T to A, chromosome 9 at 16,376,568 bp
  • A to T, chromosome 9 at 42,373,075 bp
  • A to T, chromosome 9 at 49,426,883 bp
  • T to A, chromosome 9 at 71,868,265 bp
  • T to A, chromosome 10 at 22,665,347 bp
  • A to G, chromosome 10 at 80,229,031 bp
  • C to A, chromosome 10 at 88,431,395 bp
  • G to A, chromosome 10 at 129,801,820 bp
  • C to A, chromosome 10 at 129,801,821 bp
  • T to C, chromosome 11 at 11,933,551 bp
  • A to G, chromosome 11 at 51,625,709 bp
  • G to A, chromosome 11 at 70,616,491 bp
  • A to G, chromosome 11 at 101,567,112 bp
  • T to A, chromosome 11 at 101,583,750 bp
  • A to G, chromosome 11 at 101,980,669 bp
  • A to T, chromosome 12 at 76,303,673 bp
  • A to G, chromosome 12 at 110,628,006 bp
  • C to T, chromosome 13 at 3,581,891 bp
  • C to T, chromosome 13 at 24,614,644 bp
  • G to A, chromosome 14 at 31,641,108 bp
  • T to A, chromosome 14 at 41,132,552 bp
  • G to A, chromosome 14 at 64,440,734 bp
  • A to T, chromosome 16 at 58,926,532 bp
  • T to C, chromosome 16 at 90,938,989 bp
  • A to G, chromosome 17 at 21,397,808 bp
  • A to G, chromosome 17 at 24,696,571 bp
  • A to G, chromosome 17 at 27,148,625 bp
  • G to A, chromosome 19 at 23,353,315 bp
  • T to C, chromosome 19 at 36,879,352 bp
  • T to C, chromosome 19 at 50,288,094 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6119 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044429-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.