Strain Name:
C57BL/6J-MtgxR6221Btlr/Mmmh
Stock Number:
044432-MU
Citation ID:
RRID:MMRRC_044432-MU
Other Names:
R6221 (G1)
Major Collection:

Strain Information

Slc1a6
Name: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
Synonyms: EAAT4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20513
VEGA: 10
Homologene: 21055
Abcc8
Name: ATP-binding cassette, sub-family C member 8
Synonyms: SUR1, Sur, D930031B21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
Fzd6
Name: frizzled class receptor 6
Synonyms: Fz6, rst
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14368
VEGA: 15
HGNC: HGNC:4044
Homologene: 2617
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Smc3
Name: structural maintenance of chromosomes 3
Synonyms: SmcD, Bamacan, Mmip1, Cspg6
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13006
HGNC: HGNC:2468
Homologene: 3974
Sart3
Name: squamous cell carcinoma antigen recognized by T cells 3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53890
Homologene: 40977
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Kpna1
Name: karyopherin subunit alpha 1
Synonyms: mSRP1, m-importin-alpha-S1, Rch2, NPI1, importin alpha 5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16646
HGNC: HGNC:6394
Homologene: 55642
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: Ki-67, D630048A14Rik, Ki67
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Zwilch
Name: zwilch kinetochore protein
Synonyms: 2310031L18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68014
Homologene: 32381
Dkk3
Name: dickkopf WNT signaling pathway inhibitor 3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50781
HGNC: HGNC:2893
Homologene: 8303
Mlh3
Name: mutL homolog 3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217716
VEGA: 12
HGNC: HGNC:7128
Homologene: 91153
Tank
Name: TRAF family member-associated Nf-kappa B activator
Synonyms: I-TRAF, E430026L09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21353
Homologene: 3081
Adgrl1
Name: adhesion G protein-coupled receptor L1
Synonyms: Lec2, lectomedin-2, 2900070I05Rik, Lphn1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 330814
Homologene: 8951
Thbs1
Name: thrombospondin 1
Synonyms: TSP1, TSP-1, Thbs-1, tbsp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21825
Homologene: 31142
Cftr
Name: cystic fibrosis transmembrane conductance regulator
Synonyms: Abcc7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12638
HGNC: HGNC:1884
Homologene: 55465
Vdac2
Name: voltage-dependent anion channel 2
Synonyms: Vdac6
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 22334
Homologene: 37765
Syt14
Name: synaptotagmin XIV
Synonyms: B230320I09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329324
Homologene: 17719
Hpf1
Name: histone PARylation factor 1
Synonyms: 2700029M09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72612
Homologene: 32374
Vdac3
Name: voltage-dependent anion channel 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22335
Homologene: 36115
Zfp846
Name: zinc finger protein 846
Synonyms: 2210010B09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244721
Homologene: 87243
Nol11
Name: nucleolar protein 11
Synonyms: 1500002M01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68979
Homologene: 32264
Ppip5k2
Name: diphosphoinositol pentakisphosphate kinase 2
Synonyms: Vip2, Hisppd1, Cfap160
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227399
Homologene: 49409
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: D330050P16Rik, 2010013B10Rik, A230048G03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320473
VEGA: 17
Homologene: 25536
Akap2
Name: A kinase (PRKA) anchor protein 2
Synonyms: B230340M18Rik, AKAP-KL
Type: Gene
Species: Mouse
Chromosome: 4
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Col1a-2, Cola2, Cola-2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Ppara
Name: peroxisome proliferator activated receptor alpha
Synonyms: Nr1c1, PPAR-alpha, PPARalpha, Ppar, 4933429D07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19013
HGNC: HGNC:9232
Homologene: 21047
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26875
Homologene: 69111
Yif1b
Name: Yip1 interacting factor homolog B (S. cerevisiae)
Synonyms: 9430029K10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77254
Homologene: 60114
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: Spna-1, erythroid, ihj, Spna1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20739
Homologene: 74460
Tmem116
Name: transmembrane protein 116
Synonyms: 4930513P12Rik, 4930406A18Rik, C030022K24Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77462
Homologene: 12676
Cfap44
Name: cilia and flagella associated protein 44
Synonyms: 6330444M21Rik, D16Ertd642e, Wdr52
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 212517
Homologene: 75085
Grik3
Name: glutamate receptor, ionotropic, kainate 3
Synonyms: Glur-7, Glur7
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14807
HGNC: HGNC:4581
Homologene: 73901
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Ror2
Name: receptor tyrosine kinase-like orphan receptor 2
Synonyms: Ntrkr2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26564
Homologene: 55831
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Ica1
Name: islet cell autoantigen 1
Synonyms: ICA69, 69kDa
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15893
HGNC: HGNC:5343
Homologene: 7777
Ldlrap1
Name: low density lipoprotein receptor adaptor protein 1
Synonyms: Arh1, Arh
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100017
Homologene: 9219
Gpaa1
Name: GPI anchor attachment protein 1
Synonyms: mGAA1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14731
HGNC: HGNC:4446
Homologene: 37852
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209683
Homologene: 41023
Slc7a13
Name: solute carrier family 7, (cationic amino acid transporter, y+ system) member 13
Synonyms: XAT2, AGT1, AGT-1, 0610009O04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74087
Homologene: 23656
Or5b124
Name: olfactory receptor family 5 subfamily B member 124
Synonyms: GA_x6K02T2RE5P-3965668-3966609, MOR202-19, Olfr1489
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258628
Homologene: 45021
Smco1
Name: single-pass membrane protein with coiled-coil domains 1
Synonyms: 2310010M20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 69576
Homologene: 18842
Traf1
Name: TNF receptor-associated factor 1
Synonyms: 4732496E14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22029
Homologene: 4138
Adprhl1
Name: ADP-ribosylhydrolase like 1
Synonyms: Arh2, D330008N11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234072
Homologene: 16311
Hsd3b1
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Synonyms: D3Ertd383e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 15492
Homologene: 69149
Klra10
Name: killer cell lectin-like receptor subfamily A, member 10
Synonyms: Ly49J, Ly49i2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16628
Homologene: 110821
Ppm1j
Name: protein phosphatase 1J
Synonyms: 2310008J22Rik, Ppp2cz, PP2Czeta
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71887
Homologene: 23632
Inf2
Name: inverted formin, FH2 and WH2 domain containing
Synonyms: EG629699, 2610204M08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 70435
VEGA: 12
Homologene: 82406
Gnrhr
Name: gonadotropin releasing hormone receptor
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14715
HGNC: HGNC:4421
Homologene: 350
Togaram1
Name: TOG array regulator of axonemal microtubules 1
Synonyms: A430041B07Rik, Fam179b
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 328108
VEGA: 12
Homologene: 15025
Oxct2b
Name: 3-oxoacid CoA transferase 2B
Synonyms: Scot-t2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 353371
Homologene: 137359
Taar3
Name: trace amine-associated receptor 3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 493809
HGNC: HGNC:4513
Homologene: 117490
E130311K13Rik
Name: RIKEN cDNA E130311K13 gene
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329659
Homologene: 35262
Spag1
Name: sperm associated antigen 1
Synonyms: TPR-containing protein involved in spermatogenesis, tpis
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 26942
VEGA: 15
Homologene: 8081
Odad1
Name: outer dynein arm docking complex subunit 1
Synonyms: Ccdc114
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 211535
Homologene: 128610
Rsl1d1
Name: ribosomal L1 domain containing 1
Synonyms: 2410005K20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66409
Homologene: 41052
Gimap8
Name: GTPase, IMAP family member 8
Synonyms: LOC243374, IAN9
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243374
Homologene: 69703
Evx1
Name: even-skipped homeobox 1
Synonyms: Evx-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14028
HGNC: HGNC:3506
Homologene: 1507
Hsd3b6
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6
Synonyms: 3beta-HSD VI
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 15497
Homologene: 133013
Slc15a1
Name: solute carrier family 15 (oligopeptide transporter), member 1
Synonyms: PECT1, PEPT1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56643
VEGA: 14
Homologene: 38006
Tsen34
Name: tRNA splicing endonuclease subunit 34
Synonyms: 0610027F08Rik, Leng5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66078
Homologene: 44182
Fzd4
Name: frizzled class receptor 4
Synonyms: Fz4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14366
HGNC: HGNC:4042
Homologene: 7325
Ltb4r2
Name: leukotriene B4 receptor 2
Synonyms: BLT2, 5830462O07Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57260
VEGA: 14
Homologene: 10519
Trib3
Name: tribbles pseudokinase 3
Synonyms: Trb3, Nipk, SKIP3, Ifld2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228775
Homologene: 10902
Fam240b
Name: family with sequence similarity 240 member B
Synonyms: 1190003K10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68880
Tcstv5a
Name: Tcstv family member 5A
Synonyms: Tcstv5, B020031M17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 333467
Ugt1a5
Name: UDP glucuronosyltransferase 1 family, polypeptide A5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 394433
Homologene: 88621
Spata31f1e
Name: spermatogenesis associated 31 subfamily F member 1E
Synonyms: Gm12394
Type: Gene
Species: Mouse
Chromosome: 4
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 11,266,012 bp
  • G to A, chromosome 1 at 88,166,242 bp
  • A to T, chromosome 1 at 97,730,028 bp
  • A to G, chromosome 1 at 174,181,776 bp
  • A to T, chromosome 1 at 192,930,600 bp
  • A to C, chromosome 2 at 34,948,301 bp
  • A to G, chromosome 2 at 61,650,083 bp
  • C to A, chromosome 2 at 118,119,997 bp
  • G to T, chromosome 2 at 125,320,921 bp
  • A to T, chromosome 2 at 152,338,608 bp
  • T to C, chromosome 3 at 63,915,731 bp
  • A to G, chromosome 3 at 98,806,533 bp
  • A to T, chromosome 3 at 98,853,156 bp
  • A to G, chromosome 3 at 104,785,776 bp
  • A to G, chromosome 4 at 19,839,305 bp
  • A to T, chromosome 4 at 42,793,153 bp
  • C to T, chromosome 4 at 57,855,618 bp
  • T to C, chromosome 4 at 123,116,808 bp
  • T to A, chromosome 4 at 125,705,123 bp
  • C to T, chromosome 4 at 134,757,360 bp
  • G to A, chromosome 5 at 14,675,313 bp
  • G to A, chromosome 5 at 86,185,403 bp
  • A to G, chromosome 5 at 111,271,248 bp
  • C to T, chromosome 5 at 113,743,206 bp
  • C to T, chromosome 5 at 121,491,108 bp
  • T to C, chromosome 6 at 4,539,490 bp
  • C to G, chromosome 6 at 8,644,181 bp
  • A to C, chromosome 6 at 18,282,501 bp
  • C to A, chromosome 6 at 48,463,705 bp
  • T to C, chromosome 6 at 48,658,942 bp
  • T to A, chromosome 6 at 52,316,783 bp
  • T to C, chromosome 6 at 130,269,272 bp
  • T to A, chromosome 7 at 3,695,544 bp
  • C to T, chromosome 7 at 29,245,782 bp
  • T to C, chromosome 7 at 45,947,479 bp
  • T to C, chromosome 7 at 46,175,450 bp
  • A to G, chromosome 7 at 89,404,892 bp
  • A to G, chromosome 7 at 112,121,646 bp
  • A to G, chromosome 7 at 135,697,914 bp
  • T to C, chromosome 8 at 13,225,634 bp
  • A to G, chromosome 8 at 22,588,743 bp
  • T to A, chromosome 8 at 60,893,774 bp
  • C to T, chromosome 8 at 83,937,687 bp
  • T to G, chromosome 9 at 20,593,295 bp
  • A to T, chromosome 9 at 64,161,383 bp
  • A to G, chromosome 9 at 108,105,566 bp
  • A to G, chromosome 10 at 23,950,072 bp
  • A to G, chromosome 10 at 78,800,076 bp
  • A to G, chromosome 11 at 55,296,072 bp
  • G to T, chromosome 11 at 107,171,616 bp
  • A to T, chromosome 12 at 64,966,546 bp
  • C to A, chromosome 12 at 85,268,418 bp
  • T to G, chromosome 12 at 112,603,745 bp
  • T to C, chromosome 13 at 53,113,217 bp
  • T to C, chromosome 13 at 64,481,777 bp
  • T to G, chromosome 13 at 119,949,998 bp
  • G to A, chromosome 14 at 21,845,178 bp
  • C to T, chromosome 14 at 55,762,083 bp
  • T to C, chromosome 14 at 121,464,904 bp
  • A to G, chromosome 15 at 36,197,803 bp
  • T to C, chromosome 15 at 39,030,844 bp
  • T to G, chromosome 15 at 76,333,832 bp
  • A to G, chromosome 15 at 85,777,680 bp
  • A to G, chromosome 16 at 11,201,311 bp
  • G to A, chromosome 16 at 32,273,205 bp
  • G to A, chromosome 16 at 36,020,688 bp
  • A to T, chromosome 16 at 44,437,186 bp
  • T to C, chromosome 17 at 78,766,954 bp
  • A to G, chromosome 18 at 49,871,732 bp
  • T to A, chromosome 19 at 13,633,466 bp
  • T to A, chromosome 19 at 53,641,931 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6221 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044432-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.