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Strain Name:
C57BL/6J-MtgxR6246Btlr/Mmmh
Stock Number:
044436-MU
Citation ID:
RRID:MMRRC_044436-MU
Other Names:
R6246 (G1)
Major Collection:

Strain Information

Hfe
Name: homeostatic iron regulator
Synonyms: MR2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15216
HGNC: HGNC:4886
Homologene: 88330
Trim32
Name: tripartite motif-containing 32
Synonyms: 3f3, 1810045E12Rik, Zfp117, BBS11
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69807
Homologene: 36327
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Arip4, G630026H09Rik, Srisnf2l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101772
Homologene: 75079
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: 5730502P04Rik, CAP-E, Fin16, Smc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14211
Homologene: 4705
Nisch
Name: nischarin
Synonyms: 1200007D05Rik, 3202002H23Rik, edsn
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 64652
Homologene: 136161
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 52,904,642 bp
  • A to T, chromosome 1 at 69,923,821 bp
  • T to A, chromosome 1 at 106,030,066 bp
  • C to T, chromosome 1 at 136,476,424 bp
  • G to A, chromosome 1 at 149,872,587 bp
  • A to T, chromosome 1 at 175,963,909 bp
  • T to C, chromosome 1 at 181,680,888 bp
  • G to T, chromosome 1 at 183,345,277 bp
  • C to T, chromosome 1 at 192,070,553 bp
  • G to A, chromosome 2 at 18,699,095 bp
  • A to T, chromosome 2 at 103,046,792 bp
  • G to A, chromosome 2 at 118,927,001 bp
  • T to C, chromosome 2 at 119,615,620 bp
  • A to G, chromosome 2 at 121,140,389 bp
  • T to A, chromosome 2 at 121,551,048 bp
  • G to T, chromosome 2 at 122,327,174 bp
  • A to G, chromosome 2 at 140,659,801 bp
  • T to A, chromosome 2 at 156,024,982 bp
  • T to C, chromosome 3 at 38,891,721 bp
  • C to T, chromosome 3 at 59,217,529 bp
  • A to G, chromosome 3 at 108,751,832 bp
  • A to G, chromosome 4 at 43,216,246 bp
  • A to T, chromosome 4 at 52,460,289 bp
  • A to G, chromosome 4 at 58,811,365 bp
  • T to A, chromosome 4 at 60,419,810 bp
  • T to C, chromosome 4 at 65,614,564 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • T to C, chromosome 4 at 134,089,346 bp
  • A to G, chromosome 4 at 155,888,089 bp
  • G to A, chromosome 5 at 69,558,555 bp
  • A to G, chromosome 5 at 88,662,592 bp
  • A to G, chromosome 5 at 137,985,213 bp
  • A to T, chromosome 5 at 146,170,240 bp
  • G to A, chromosome 6 at 43,167,502 bp
  • A to T, chromosome 6 at 55,967,672 bp
  • A to G, chromosome 6 at 89,651,541 bp
  • G to A, chromosome 6 at 120,431,910 bp
  • A to T, chromosome 6 at 129,353,770 bp
  • T to A, chromosome 7 at 19,369,858 bp
  • C to T, chromosome 7 at 25,271,642 bp
  • A to G, chromosome 7 at 45,936,364 bp
  • T to A, chromosome 7 at 122,169,436 bp
  • T to C, chromosome 7 at 125,576,405 bp
  • T to A, chromosome 7 at 126,827,496 bp
  • T to C, chromosome 7 at 144,633,725 bp
  • TCGACG to TCG, chromosome 8 at 71,890,708 bp
  • A to G, chromosome 8 at 90,932,417 bp
  • C to T, chromosome 8 at 105,849,259 bp
  • A to T, chromosome 9 at 18,577,067 bp
  • A to G, chromosome 9 at 25,307,521 bp
  • T to C, chromosome 9 at 42,377,908 bp
  • A to G, chromosome 9 at 106,700,493 bp
  • C to A, chromosome 9 at 115,226,774 bp
  • T to A, chromosome 10 at 80,015,165 bp
  • C to T, chromosome 11 at 28,576,897 bp
  • C to A, chromosome 11 at 84,315,970 bp
  • T to C, chromosome 11 at 87,822,232 bp
  • G to A, chromosome 12 at 104,317,447 bp
  • A to T, chromosome 13 at 23,708,229 bp
  • G to A, chromosome 13 at 24,466,790 bp
  • T to C, chromosome 13 at 34,990,198 bp
  • T to C, chromosome 13 at 54,719,710 bp
  • T to C, chromosome 14 at 31,172,559 bp
  • A to T, chromosome 14 at 51,456,046 bp
  • G to A, chromosome 14 at 53,977,428 bp
  • C to T, chromosome 14 at 55,636,370 bp
  • T to A, chromosome 14 at 67,677,828 bp
  • T to C, chromosome 14 at 70,467,055 bp
  • A to T, chromosome 14 at 101,918,700 bp
  • T to A, chromosome 15 at 8,210,014 bp
  • T to C, chromosome 15 at 101,780,937 bp
  • A to G, chromosome 16 at 17,895,439 bp
  • T to A, chromosome 16 at 26,714,881 bp
  • G to A, chromosome 16 at 31,665,650 bp
  • A to G, chromosome 16 at 50,224,660 bp
  • C to A, chromosome 17 at 23,710,491 bp
  • T to C, chromosome 17 at 26,543,678 bp
  • A to T, chromosome 17 at 40,207,424 bp
  • T to C, chromosome 17 at 57,025,324 bp
  • A to G, chromosome 17 at 69,387,483 bp
  • A to G, chromosome 17 at 88,671,547 bp
  • T to C, chromosome 19 at 3,376,550 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6246 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044436-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.