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Strain Name:
C57BL/6J-MtgxR6268Btlr/Mmmh
Stock Number:
044439-MU
Citation ID:
RRID:MMRRC_044439-MU
Other Names:
R6268 (G1)
Major Collection:

Strain Information

Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Frrs1
Name: ferric-chelate reductase 1
Synonyms: Sdfr2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20321
Homologene: 40653
Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Rapgef6
Name: Rap guanine nucleotide exchange factor (GEF) 6
Synonyms: PDZ-GEF2, RA-GEF-2, Pdzgef2, C030018K18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192786
Homologene: 22968
Ahctf1
Name: AT hook containing transcription factor 1
Synonyms: 6230412P20Rik, Elys
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226747
Homologene: 9142
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 133,624,557 bp
  • C to T, chromosome 1 at 163,946,217 bp
  • C to A, chromosome 1 at 167,078,553 bp
  • T to C, chromosome 1 at 179,763,483 bp
  • C to T, chromosome 1 at 193,198,036 bp
  • A to G, chromosome 2 at 19,540,408 bp
  • A to G, chromosome 2 at 25,903,597 bp
  • T to A, chromosome 2 at 40,821,717 bp
  • G to T, chromosome 2 at 74,698,089 bp
  • T to C, chromosome 2 at 90,863,648 bp
  • C to A, chromosome 3 at 93,288,175 bp
  • G to T, chromosome 3 at 107,747,157 bp
  • T to C, chromosome 3 at 109,935,035 bp
  • G to T, chromosome 3 at 116,903,099 bp
  • T to C, chromosome 4 at 56,762,305 bp
  • G to T, chromosome 4 at 118,569,451 bp
  • G to A, chromosome 4 at 141,632,341 bp
  • A to T, chromosome 5 at 24,756,191 bp
  • A to C, chromosome 5 at 87,329,613 bp
  • A to G, chromosome 5 at 97,392,210 bp
  • A to C, chromosome 5 at 109,221,417 bp
  • C to T, chromosome 5 at 135,643,260 bp
  • A to G, chromosome 6 at 30,615,173 bp
  • T to A, chromosome 6 at 48,977,682 bp
  • C to T, chromosome 6 at 113,392,563 bp
  • A to G, chromosome 6 at 121,779,073 bp
  • G to A, chromosome 7 at 29,143,068 bp
  • A to T, chromosome 7 at 33,364,548 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • TCCCC to TCCC, chromosome 8 at 11,529,599 bp
  • A to T, chromosome 8 at 23,109,671 bp
  • A to T, chromosome 8 at 70,888,620 bp
  • A to G, chromosome 8 at 94,850,234 bp
  • A to G, chromosome 8 at 119,870,667 bp
  • A to T, chromosome 8 at 123,492,926 bp
  • T to G, chromosome 9 at 7,447,622 bp
  • T to C, chromosome 9 at 38,378,088 bp
  • T to C, chromosome 9 at 44,317,666 bp
  • C to A, chromosome 9 at 67,951,449 bp
  • T to A, chromosome 9 at 95,964,014 bp
  • T to G, chromosome 9 at 109,654,650 bp
  • T to A, chromosome 10 at 93,038,909 bp
  • A to T, chromosome 10 at 95,496,707 bp
  • T to A, chromosome 11 at 5,019,493 bp
  • T to A, chromosome 11 at 54,649,247 bp
  • G to T, chromosome 11 at 54,987,077 bp
  • T to C, chromosome 11 at 60,712,163 bp
  • T to A, chromosome 11 at 90,540,201 bp
  • G to A, chromosome 12 at 84,331,838 bp
  • T to A, chromosome 12 at 87,081,953 bp
  • T to C, chromosome 12 at 103,316,544 bp
  • C to A, chromosome 12 at 108,692,580 bp
  • G to A, chromosome 13 at 19,336,831 bp
  • G to T, chromosome 13 at 58,013,842 bp
  • G to A, chromosome 13 at 60,761,766 bp
  • A to G, chromosome 13 at 67,542,586 bp
  • T to C, chromosome 14 at 54,989,284 bp
  • C to A, chromosome 14 at 67,790,275 bp
  • A to T, chromosome 16 at 32,768,767 bp
  • A to C, chromosome 16 at 59,167,293 bp
  • A to G, chromosome 16 at 90,753,919 bp
  • C to A, chromosome 17 at 49,435,155 bp
  • G to A, chromosome 17 at 56,771,587 bp
  • T to C, chromosome 17 at 88,742,704 bp
  • T to A, chromosome 18 at 12,524,737 bp
  • C to A, chromosome 18 at 20,388,163 bp
  • T to C, chromosome 18 at 35,619,571 bp
  • G to A, chromosome 18 at 37,022,424 bp
  • A to T, chromosome 19 at 13,815,307 bp
  • A to T, chromosome 19 at 43,739,926 bp
  • T to A, chromosome 19 at 48,790,166 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6268 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044439-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.