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Strain Name:
C57BL/6J-MtgxR6269Btlr/Mmmh
Stock Number:
044440-MU
Citation ID:
RRID:MMRRC_044440-MU
Other Names:
R6269 (G1)
Major Collection:

Strain Information

Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Lrrc2
Name: leucine rich repeat containing 2
Synonyms: 2400002D05Rik, 4933431K03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74249
Homologene: 23454
Ap2b1
Name: adaptor-related protein complex 2, beta 1 subunit
Synonyms: 1300012O03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71770
HGNC: HGNC:563
Homologene: 137384
Sde2
Name: SDE2 telomere maintenance homolog (S. pombe)
Synonyms: BC031781
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 208768
Homologene: 134327
Kif5b
Name: kinesin family member 5B
Synonyms: Khc, kinesin heavy chain
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16573
HGNC: HGNC:6324
Homologene: 55829
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 54,548,008 bp
  • G to A, chromosome 1 at 180,855,806 bp
  • G to A, chromosome 1 at 189,659,920 bp
  • A to T, chromosome 2 at 87,846,874 bp
  • T to C, chromosome 2 at 104,907,633 bp
  • T to A, chromosome 2 at 180,281,512 bp
  • A to G, chromosome 3 at 59,227,822 bp
  • A to G, chromosome 3 at 106,204,171 bp
  • T to C, chromosome 3 at 107,749,001 bp
  • T to C, chromosome 3 at 142,312,325 bp
  • A to G, chromosome 4 at 115,491,131 bp
  • T to A, chromosome 4 at 115,524,312 bp
  • T to C, chromosome 4 at 149,644,067 bp
  • A to T, chromosome 4 at 154,037,722 bp
  • C to T, chromosome 5 at 14,522,094 bp
  • T to A, chromosome 5 at 14,912,260 bp
  • A to T, chromosome 5 at 129,766,465 bp
  • A to G, chromosome 6 at 42,627,408 bp
  • T to A, chromosome 6 at 86,964,051 bp
  • T to A, chromosome 6 at 126,886,727 bp
  • C to A, chromosome 6 at 147,092,307 bp
  • G to T, chromosome 7 at 6,393,613 bp
  • G to A, chromosome 7 at 58,803,739 bp
  • G to A, chromosome 7 at 80,309,427 bp
  • GGCG to GGCGACGGCCGCG, chromosome 7 at 97,579,906 bp
  • A to G, chromosome 7 at 97,974,462 bp
  • T to C, chromosome 7 at 99,917,472 bp
  • A to T, chromosome 7 at 104,860,350 bp
  • T to A, chromosome 7 at 127,330,217 bp
  • T to A, chromosome 7 at 128,206,265 bp
  • T to A, chromosome 7 at 140,946,184 bp
  • A to G, chromosome 8 at 14,822,369 bp
  • T to A, chromosome 8 at 105,395,360 bp
  • G to A, chromosome 9 at 35,561,184 bp
  • G to A, chromosome 9 at 59,650,012 bp
  • A to G, chromosome 9 at 110,980,949 bp
  • A to T, chromosome 9 at 122,261,242 bp
  • G to A, chromosome 10 at 91,117,101 bp
  • T to A, chromosome 11 at 5,978,497 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • T to G, chromosome 11 at 33,623,916 bp
  • A to G, chromosome 11 at 70,598,987 bp
  • A to G, chromosome 11 at 83,346,673 bp
  • A to G, chromosome 11 at 94,044,507 bp
  • T to C, chromosome 12 at 55,255,972 bp
  • T to C, chromosome 12 at 85,332,329 bp
  • C to T, chromosome 13 at 3,581,891 bp
  • A to T, chromosome 13 at 13,363,511 bp
  • A to G, chromosome 13 at 119,766,405 bp
  • T to A, chromosome 14 at 70,137,162 bp
  • T to C, chromosome 14 at 99,073,667 bp
  • T to A, chromosome 15 at 80,880,743 bp
  • A to G, chromosome 15 at 89,160,713 bp
  • T to A, chromosome 17 at 18,166,774 bp
  • G to A, chromosome 17 at 28,881,368 bp
  • A to G, chromosome 18 at 6,223,558 bp
  • T to C, chromosome 18 at 77,948,370 bp
  • A to G, chromosome 19 at 12,588,443 bp
  • T to A, chromosome 19 at 44,029,187 bp
  • T to A, chromosome 19 at 46,719,952 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6269 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044440-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.